Epigentek

Epigentek Chromoplasts Chromatin Extraction Kit

Product Overview

The ChromaFlash Chromatin Extraction Package is a whole set of optimized buffers and reagents for isolating chromatin or DNA-protein advanced from mammalian cells or tissues in a easy and fast format. Chromatin ready by this package can be utilized in quite a lot of chromatin immunoprecipitation strategies. Additionally it is the really useful methodology for acquiring chromatin required by Epigentek’s one-hour ChIP methodology utilizing the ChromaFlash One-Step ChIP Package. The remoted chromatin will also be utilized in different chromatin-related functions equivalent to in vitro protein-DNA binding assays and nuclear enzyme assays.

  • Extraordinarily quick process: your entire process from cell/tissue pattern to ready-to-use chromatin is lower than 60 minutes.
  • Handy and versatile: the package is appropriate for making ready each native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
  • Unsheared chromatin makes it customizable for numerous evaluation workflows that require both intact or fragmented chromatin, together with ChIP, in vitro protein-DNA interplay evaluation, nuclear enzyme assay, and so forth.
epigentek chromoplasts chromatin
epigentek chromoplasts chromatin

Background Data
Chromatin immunoprecipitaton (ChIP) gives an advantageous device for finding out protein-DNA interplay. With ChIP, the experimenter can decide if a particular protein binds to the precise sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) methods. For instance, the measurement of the quantity of methylated histone H3 at lysine 9 (meH3-K9) related to a particular gene promoter area below numerous situations could be achieved by means of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could be detected by ChIP-chip. Particularly, the ChIP methodology with particular antibodies straight towards numerous transcriptional components is broadly demanded.

Precept & Process
The ChromaFlash™ Chromatin Extraction Package accommodates all reagents required for finishing up profitable chromatin extraction straight from mammalian cells or tissues. Cell membranes of the pattern, with or with out cross-linking, are damaged down utilizing the supplied lysis buffer. Chromatin or DNA-protein advanced is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the applicable temperature.

Beginning Supplies & Enter Quantity
Beginning supplies can embrace numerous tissue or cell samples equivalent to cells from flask or microplate cultured cells, contemporary and frozen tissues, and so forth. The quantity of cells and tissues for every preparation could be 1 x 105 to five x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter quantity ought to be 1 to five x 106 cells or 50 to 200 mg tissues.  Yield of chromatin is roughly four ug per 106  cells or per 50 mg tissues.

Background Data
Chromatin immunoprecipitation (ChIP) gives an advantageous device for finding out protein-DNA interplay. With ChIP, the experimenter can decide if a particular protein binds to the precise sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) methods. For instance, the measurement of the quantity of methylated histone H3 at lysine 9 (meH3-K9) related to a particular gene promoter area below numerous situations could be achieved by means of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could be detected by ChIP-chip. Particularly, a ChIP methodology with particular antibodies straight towards numerous transcriptional components is broadly in demand.

Precept & Process
The ChromaFlash™ Plant Chromatin Extraction Package accommodates all of the reagents required for finishing up a profitable chromatin extraction straight from vegetation. Cell membranes of the pattern, with or with out cross-linking, are damaged down utilizing the supplied lysis buffer. Chromatin or DNA-protein advanced is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the applicable temperature.

ChromaFlash Plant Chromatin Extraction Kit 

P-2022-050 50 Extractions
EUR 193.6

ChromaFlash Plant Chromatin Extraction Kit 

P-2022
  • EUR 331.44
  • EUR 193.60
  • 50 Extractions
  • 50 Extractions

miRNA Extraction Kit

K1456-50 50 Preps
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ExoDNA? Extraction Kit

K1242-25 each
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ExoDNA? Extraction Kit

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Total Protein Extraction Kit

BSP003 50Preps
EUR 130.99

Plant Protein Extraction Kit

BSP004 20Preps
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Nuclear Protein Extraction Kit

BSP009 50Preps
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FFPE Protein Extraction Kit

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Membrane Protein Extraction Kit

55R-1357 50 assays
EUR 888
Description: Membrane Protein Extraction Kit for use in the research laboratory

Mammalian Cell Extraction Kit

55R-1358 500 assays
EUR 457.2
Description: Mammalian Cell Extraction Kit for use in the research laboratory

Quick Gel Extraction Kit

20-abx098085
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  • 200 rxns
  • 50 rxns

Up RNA Extraction Kit

20-abx098087
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  • EUR 126.00
  • 100 ml
  • 3 ml

Plant RNA extraction Kit

20-abx098088
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml

Total Protein Extraction Kit

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EUR 444
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein Extraction Kit

K2113-50 50 assays
EUR 652.8

Mammalian Cell Extraction Kit

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EUR 385.2

Bacterial RNA Extraction Kit

R403-01 100 rxn
EUR 186

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