Epigentek

Epigentek Chromoplasts Chromatin Extraction Kit

Product Overview

The ChromaFlash Chromatin Extraction Package is a whole set of optimized buffers and reagents for isolating chromatin or DNA-protein advanced from mammalian cells or tissues in a easy and fast format. Chromatin ready by this package can be utilized in quite a lot of chromatin immunoprecipitation strategies. Additionally it is the really useful methodology for acquiring chromatin required by Epigentek’s one-hour ChIP methodology utilizing the ChromaFlash One-Step ChIP Package. The remoted chromatin will also be utilized in different chromatin-related functions equivalent to in vitro protein-DNA binding assays and nuclear enzyme assays.

  • Extraordinarily quick process: your entire process from cell/tissue pattern to ready-to-use chromatin is lower than 60 minutes.
  • Handy and versatile: the package is appropriate for making ready each native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
  • Unsheared chromatin makes it customizable for numerous evaluation workflows that require both intact or fragmented chromatin, together with ChIP, in vitro protein-DNA interplay evaluation, nuclear enzyme assay, and so forth.
epigentek chromoplasts chromatin
epigentek chromoplasts chromatin

Background Data
Chromatin immunoprecipitaton (ChIP) gives an advantageous device for finding out protein-DNA interplay. With ChIP, the experimenter can decide if a particular protein binds to the precise sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) methods. For instance, the measurement of the quantity of methylated histone H3 at lysine 9 (meH3-K9) related to a particular gene promoter area below numerous situations could be achieved by means of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could be detected by ChIP-chip. Particularly, the ChIP methodology with particular antibodies straight towards numerous transcriptional components is broadly demanded.

Precept & Process
The ChromaFlash™ Chromatin Extraction Package accommodates all reagents required for finishing up profitable chromatin extraction straight from mammalian cells or tissues. Cell membranes of the pattern, with or with out cross-linking, are damaged down utilizing the supplied lysis buffer. Chromatin or DNA-protein advanced is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the applicable temperature.

Beginning Supplies & Enter Quantity
Beginning supplies can embrace numerous tissue or cell samples equivalent to cells from flask or microplate cultured cells, contemporary and frozen tissues, and so forth. The quantity of cells and tissues for every preparation could be 1 x 105 to five x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter quantity ought to be 1 to five x 106 cells or 50 to 200 mg tissues.  Yield of chromatin is roughly four ug per 106  cells or per 50 mg tissues.

Background Data
Chromatin immunoprecipitation (ChIP) gives an advantageous device for finding out protein-DNA interplay. With ChIP, the experimenter can decide if a particular protein binds to the precise sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) methods. For instance, the measurement of the quantity of methylated histone H3 at lysine 9 (meH3-K9) related to a particular gene promoter area below numerous situations could be achieved by means of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could be detected by ChIP-chip. Particularly, a ChIP methodology with particular antibodies straight towards numerous transcriptional components is broadly in demand.

Precept & Process
The ChromaFlash™ Plant Chromatin Extraction Package accommodates all of the reagents required for finishing up a profitable chromatin extraction straight from vegetation. Cell membranes of the pattern, with or with out cross-linking, are damaged down utilizing the supplied lysis buffer. Chromatin or DNA-protein advanced is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the applicable temperature.

ChromaFlash Chromatin Isolation & Shearing Kit 

P-2023
  • EUR 324.48
  • EUR 189.20
  • 100 Preperations
  • 100 Preparations

EpiQuik Chromatin Accessibility Assay Kit 

P-1047
  • EUR 910.86
  • EUR 611.60
  • 48 Assays
  • 48 Reactions

miRNA Extraction Kit

K1456-50 50 Preps
EUR 661.2

ExoDNA? Extraction Kit

K1242-25 each
EUR 705.6

ExoDNA? Extraction Kit

K1242-50 each
EUR 1057.2

Up RNA Extraction Kit

20-abx098087
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit 

P-2002
  • EUR 1084.86
  • EUR 312.40
  • EUR 500.50
  • EUR 738.10
  • 96 Reactions
  • 24 Reactions
  • 48 Reactions
  • 96 Reactions

Quick Gel Extraction Kit

20-abx098085
  • EUR 343.20
  • EUR 243.60
  • 200 rxns
  • 50 rxns

Plant RNA extraction Kit

20-abx098088
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml

Chromatin protein Cren7 Antibody (HRP)

20-abx109204
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Chromatin protein Cren7 Antibody (FITC)

20-abx107787
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Gel Extraction Kit (100 preps)

9K-006-0001 100preps
EUR 283.42

Gel Extraction Kit (200 preps)

9K-006-0002 200preps, 200prep
EUR 418.09

FFPE Protein Extraction Kit

BSP058 50Preps
EUR 122.64

Exosomal RNA Extraction Kit

P200R,-part-of-P200 - Ask for price

Exosomal DNA Extraction Kit

P230-25 - Ask for price

Exosomal DNA Extraction Kit

P230-50 - Ask for price

Plant Tissue Extraction Kit

K296-50 each
EUR 548.4

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