Our product Cell Counting Package-8 (CCK-8) present a extra handy and delicate technique for the analysis of cell quantity dedication and cell proliferationtotoxicity assay than earlier methods. The equipment makes use of a extremely water-soluble tetrazolium salt, WST-8, which produces a water-soluble formazan dye upon discount within the presence of an electron mediator. The quantity of the formazan generated by dehydrogenases is straight in proportion to the numbers of residing cells. The detection sensitivity by CCK-Eight is greater than different tetrazolium salts resembling MTT, XTT, MTS or WST-1.
Options
Extra Delicate than MTT, MTS, or WST-1
No Toxicity to Cell
Less complicated Steps, No natural solvents required
Steady One Bottle Answer, ready-to-use
Apexbio Cell Counting Package-8
Background
Cell proliferation will be detected by quite a lot of strategies. Cell proliferation induces a rise within the exercise of the mitochondrial dehydrogenases, which cleaves the tetrazolium salt to formazan.
The WST Cell Proliferation Colorimetric Assay Package plus supplies the best and most delicate method for quantification of cell proliferation and viability. Cell proliferation causes the rise within the quantity of formazan dye fashioned that’s straight proportional to the variety of residing cells and will be quantified by measuring the absorbance of the dye resolution at 440 nm utilizing microtiter plate reader. The equipment can be utilized for the evaluation of cell proliferation in response to pharmaceutical compounds, cytotoxic compounds like anticancer medicine and lots of different poisonous brokers. The assay may also be used for the detection of cell proliferation in response to cytokines, mitogens, progress elements and vitamins, and so forth. The assay could be very straightforward and easy, simply add-and-read, requiring no solubilization, no washing and no harvesting steps, and is steady, sooner and extra delicate than MTS, MTT or XTT-based assays. Your entire assay will be carried out in a microtiter plate and correlates nicely with the [3H]-thymidine incorporation assay.
Cell Counting Package-Eight Apexbio
Options & Properties
Options
Quick and handy Easy one-step process; takes lower than Four hours. Makes use of a water-soluble tetrazolium salt WST reagent. The strategy is so easy that requires no washing/harvesting/or solubilization steps and is quicker and extra delicate than MTT/XTT/or MTS-based assays. Your entire assay will be carried out straight in a 96-well plate.
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.