Equipments

Scientists Identify Corona virus Strain

Scientists Identify Corona virus Strain In Cyprus-‘Deltacron’-That Blends Delta And Omicron Scientists in Cyprus have found 25 cases of a strain of the coronavirus that they say combines elements of the delta and omicron variants, dubbing it “deltacron,” with a high proportion of the variant found in patients hospitalized for Covid-19 People at the Larnaca International Airport in Cyprus (Photo by […]

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COVID-19: Viral Transport Medium (VTM) for Diagnosis

The success of diagnosis of SARS-CoV-2 (2019-nCoV) during the COVID-19 outbreak largely depends on the quality of the sample and the conditions under which the sample is transported and stored before being processed in the laboratory. Viral transport medium (VTM) allows the safe transfer of viruses, chlamydia and mycoplasma for further research, which includes conventional

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Agrobacterium Strains

Almost 40 years ago the first transgenic plant was generated through Agrobacterium tumefaciens-mediated transformation, which, until now, remains the method of choice for gene delivery into plants. Ever since, optimized Agrobacterium strains have been developed with additional (genetic) modifications that were mostly aimed at enhancing the transformation efficiency, although an optimized strain also exists that reduces unwanted plasmid recombination.

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A Chemically Defined Common Medium for Culture of C2C12 Skeletal Muscle and Human Induced Pluripotent Stem Cell Derived Spinal Spheroids

A Chemically Defined Common Medium for Culture of C2C12 Skeletal Muscle and Human Induced Pluripotent Stem Cell Derived Spinal Spheroids

Introduction: Multicellular platforms and linked multi organ on chip gadgets are highly effective instruments for drug discovery, and primary mechanistic research. Typically, a crucial constraint is defining a tradition medium optimum for all cells current within the system. On this examine, we targeted on the important thing cells of the neuromuscular junction i.e., skeletal muscle and

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Culture of rabbit caecum organoids by reconstituting the intestinal stem cell niche in vitro with pharmacological inhibitors or L-WRN conditioned medium

Culture of rabbit caecum organoids by reconstituting the intestinal stem cell niche in vitro with pharmacological inhibitors or L-WRN conditioned medium

Intestinal organoids are self-organized three-d (3D) constructions shaped by a single layer of polarized epithelial cells. This revolutionary in vitro mannequin is very related to check physiology of the intestinal epithelium and its function in vitamin and barrier perform. Nonetheless, this mannequin has by no means been developed in rabbits, whereas it could have potential

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A Microfluidic System for One-Chip Harvesting of Single-Cell-Laden Hydrogels in Culture Medium

A Microfluidic System for One-Chip Harvesting of Single-Cell-Laden Hydrogels in Culture Medium

Single-cell evaluation has proven nice potential to totally quantify the distribution of mobile behaviors amongst a inhabitants of people. By isolation and preservation of single cells within the aqueous part, droplet encapsulation adopted by gelation allows high-throughput evaluation in biocompatible microgels. Nonetheless, the shortage of management over the variety of cells encapsulated and complex gelation

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Isolation and Characterization of Extracellular Vesicles from Cell Culture Conditioned Medium for Immunological Studies

Extracellular vesicles (EVs) are small, membranous particles which have just lately emerged as one a very powerful mediators of intercellular communication. They will comprise quite a lot of proteins, lipids, and nucleic acids and thus are answerable for modulation of a number of organic processes, together with immune response and regulation of immune cells. Immunomodulatory

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Is cell-free DNA in spent embryo culture medium an alternative to embryo biopsy for preimplantation genetic testing? a systematic review.

Preimplantation genetic testing (PGT) is more and more used worldwide. It presently entails using invasive strategies, i.e. polar physique, blastomere, trophectoderm biopsy or blastocentesis, to acquire embryonic DNA, with main technical limitations and moral points. Proof means that invasive PGT can result in genetic misdiagnosis within the case of embryo mosaicism, and, consequently, to the

Is cell-free DNA in spent embryo culture medium an alternative to embryo biopsy for preimplantation genetic testing? a systematic review. Read More »