Extracellular vesicles (EVs) are small, membranous particles which have just lately emerged as one a very powerful mediators of intercellular communication. They will comprise quite a lot of proteins, lipids, and nucleic acids and thus are answerable for modulation of a number of organic processes, together with immune response and regulation of immune cells. Immunomodulatory exercise of various EVs might be reliably assessed utilizing EVs remoted from cell tradition conditioned medium and added to in vitro or ex vivo cultures of immune cells. This text describes protocols for isolation of EVs from cell tradition supernatants by differential ultracentrifugation and density gradient centrifugation. It additionally offers instruments and protocols that allow characterization and validation of remoted particles, in addition to evaluation of interactions between EVs of curiosity and totally different subpopulations of human immune cells.
2020 Wiley Periodicals LLC. Fundamental Protocol 1: Isolation of extracellular vesicles by differential ultracentrifugation Fundamental Protocol 2: Isolation of extracellular vesicles by density gradient centrifugation Help Protocol 1: Imaging of extracellular vesicles utilizing transmission electron microscopy Help Protocol 2: Detection of extracellular vesicle protein markers by Western blotting Help Protocol 3: Measurement and counting of extracellular vesicles by nanoparticle monitoring evaluation Fundamental Protocol 3: Evaluation of extracellular vesicle uptake or affiliation by totally different subpopulations of lymphocytes in vitro.
Simultaneous Quantification of Chosen Kynurenines Analyzed by Liquid Chromatography-Mass Spectrometry in Medium Collected From Most cancers Cell Cultures
The kynurenine pathway and the tryptophan catabolites known as kynurenines have obtained elevated consideration for his or her involvement in immune regulation and most cancers biology. An in vitro cell tradition assay is commonly used to be taught in regards to the contribution of various tryptophan catabolites in a illness mechanism and for testing therapeutic methods. Cell tradition medium that’s wealthy in secreted metabolites and signaling molecules displays the standing of tryptophan metabolism and different mobile occasions. New protocols for the dependable quantification of a number of kynurenines within the complicated cell tradition medium are desired to permit for a dependable and fast evaluation of a number of samples.
This may be completed with liquid chromatography coupled with mass spectrometry. This highly effective method is employed in lots of scientific and analysis laboratories for the quantification of metabolites and can be utilized for measuring kynurenines. Offered right here is the usage of liquid chromatography coupled with single quadrupole mass spectrometry (LC-SQ) for the simultaneous willpower of 4 kynurenines, i.e., kynurenine, 3-hydroxykynurenine, 3-hydroxyanthranilic, and xanthurenic acid within the medium collected from in vitro cultured most cancers cells. SQ detector is easy to make use of and cheaper in comparison with different mass spectrometers. Within the SQ-MS evaluation, a number of ions from the pattern are generated and separated based on their particular mass-to-charge ratio (m/z), adopted by the detection utilizing a Single Ion Monitoring (SIM) mode.
This paper attracts the eye on some great benefits of the reported technique and signifies some weak factors. It’s centered on vital parts of LC-SQ evaluation together with pattern preparation together with chromatography and mass spectrometry evaluation. The standard management, technique calibration situations and matrix impact points are additionally mentioned. We described a easy software of 3-nitrotyrosine as one analog customary for all goal analytes. As confirmed by experiments with human ovary and breast most cancers cells, the proposed LC-SQ technique generates dependable outcomes and might be additional utilized to different in vitro mobile fashions.
Direct counting of exosomes in a cell tradition medium utilizing neither isolation nor preconcentration
Exosomes are anticipated to be biomarkers of most cancers since they comprise details about the cells that excrete them. On this examine we developed a way to depend the exosomes secreted from most cancers cells in a tradition medium with out the necessity for isolation and/or preconcentration. This detection system consists of a sq. capillary on which a laser beam is concentrated in a sheet form by way of the usage of two cylindrical lenses. A fluorescently labeled anti-CD63 antibody is used to mark the exosomes which can be then flowed into the sq. capillary.
Chikungunya virus PCR kit |
PCR-H525-96R |
Bioingentech |
100T |
EUR 1335.6 |
Chikungunya virus One-Step PCR kit |
Oneq-H525-100R |
Bioingentech |
100T |
EUR 1932 |
Chikungunya virus One-Step PCR kit |
Oneq-H525-150R |
Bioingentech |
150T |
EUR 2646 |
Chikungunya virus One-Step PCR kit |
Oneq-H525-50R |
Bioingentech |
50T |
EUR 1410 |
Chikungunya virus antibody |
10-2716 |
Fitzgerald |
500 ug |
EUR 418.8 |
Description: Mouse monoclonal Chikungunya virus envelope antibody |
Chikungunya virus antibody |
10-2717 |
Fitzgerald |
500 ug |
EUR 418.8 |
Description: Mouse monoclonal Chikungunya virus envelope antibody |
Chikungunya virus antibody |
10-2718 |
Fitzgerald |
500 ug |
EUR 418.8 |
Description: Mouse monoclonal Chikungunya virus envelope antibody |
Chikungunya virus antibody |
10-2719 |
Fitzgerald |
250 ug |
EUR 454.8 |
Description: Mouse monoclonal Chikungunya virus antibody |
Chikungunya virus antibody |
10-2720 |
Fitzgerald |
250 ug |
EUR 454.8 |
Description: Mouse monoclonal Chikungunya virus antibody |
Chikungunya Virus Antibody |
abx021574-1mg |
Abbexa |
1 mg |
EUR 1296 |
|
Chikungunya Virus Antibody |
abx021575-1mg |
Abbexa |
1 mg |
EUR 1296 |
|
Chikungunya Virus Antibody |
abx021576-1mg |
Abbexa |
1 mg |
EUR 1296 |
|
Chikungunya virus E1 protein |
30-1942 |
Fitzgerald |
100 ug |
EUR 418.8 |
Description: Recombinant Chikungunya virus E1 protein - wild type |
Chikungunya virus gpE2 protein |
30-1939 |
Fitzgerald |
100 ug |
EUR 457.2 |
Description: Recombinant Chikungunya virus Glycoprotein E2 protein - wild type |
Chikungunya virus gpE1 protein |
30-1940 |
Fitzgerald |
100 ug |
EUR 457.2 |
Description: Recombinant Chikungunya virus Glycoprotein E1 protein - wild type |
Recombinant Chikungunya Virus E1 |
P1113-10 |
Biovision |
each |
EUR 614.4 |
Recombinant Chikungunya Virus (CHIKV) |
4-RPX256Ge01 |
Cloud-Clone |
-
EUR 711.36
-
EUR 314.40
-
EUR 2337.60
-
EUR 859.20
-
EUR 1598.40
-
EUR 552.00
-
EUR 5664.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Pan-species Chikungunya Virus expressed in: E.coli |
Chikungunya Virus Envelope Antibody |
abx024072-1mg |
Abbexa |
1 mg |
EUR 1161.6 |
|
Chikungunya Virus Envelope Antibody |
abx024073-1mg |
Abbexa |
1 mg |
EUR 1161.6 |
|
Chikungunya Virus Envelope Antibody |
abx024074-1mg |
Abbexa |
1 mg |
EUR 1161.6 |
|
Chikungunya Virus Envelope Antibody |
abx024075-1mg |
Abbexa |
1 mg |
EUR 1161.6 |
|
Chikungunya Virus Envelope Antibody |
abx024076-1mg |
Abbexa |
1 mg |
EUR 1161.6 |
|
Mouse antibody for Chikungunya virus |
3584 |
Virostat |
100 ug |
EUR 386.26 |
Description: This is purified Mouse monoclonal antibody against Chikungunya virus for WB, ELISA. |
OVA Conjugated Chikungunya Virus (CHIKV) |
4-CPX256Ge21 |
Cloud-Clone |
-
EUR 711.36
-
EUR 314.40
-
EUR 2337.60
-
EUR 859.20
-
EUR 1598.40
-
EUR 552.00
-
EUR 5664.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Pan-species Chikungunya Virus expressed in: Protein conjugation |
Human Chikungunya Virus IgG (CHIKV-IgG) ELISA Kit |
abx054994-96tests |
Abbexa |
96 tests |
EUR 801.6 |
|
Human Chikungunya Virus IgM (CHIKV-IgM) ELISA Kit |
abx055296-96tests |
Abbexa |
96 tests |
EUR 801.6 |
|
Chikungunya virus E1 protein (A226V mutant) |
30-1943 |
Fitzgerald |
100 ug |
EUR 418.8 |
Description: Recombinant Chikungunya virus E1 protein - A266V mutant |
Chikungunya virus gpE1 protein (A226V mutant) |
30-1941 |
Fitzgerald |
100 ug |
EUR 457.2 |
Description: Recombinant Chikungunya virus Glycoprotein E1 protein - A226V mutant |
ELISA kit for Human Chikungunya Virus IgG (CHIKV IgG) Kit |
KTE62972-48T |
Abbkine |
48T |
EUR 424.8 |
Description: Quantitative sandwich ELISA for measuring Human Chikungunya Virus IgG (CHIKV IgG) Kit in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Chikungunya Virus IgG (CHIKV IgG) Kit |
KTE62972-5platesof96wells |
Abbkine |
5 plates of 96 wells |
EUR 2702.4 |
Description: Quantitative sandwich ELISA for measuring Human Chikungunya Virus IgG (CHIKV IgG) Kit in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Chikungunya Virus IgG (CHIKV IgG) Kit |
KTE62972-96T |
Abbkine |
96T |
EUR 686.4 |
Description: Quantitative sandwich ELISA for measuring Human Chikungunya Virus IgG (CHIKV IgG) Kit in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Chikungunya Virus IgM (CHIKV IgM) Kit |
KTE62973-48T |
Abbkine |
48T |
EUR 424.8 |
Description: Quantitative sandwich ELISA for measuring Human Chikungunya Virus IgM (CHIKV IgM) Kit in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Chikungunya Virus IgM (CHIKV IgM) Kit |
KTE62973-5platesof96wells |
Abbkine |
5 plates of 96 wells |
EUR 2702.4 |
Description: Quantitative sandwich ELISA for measuring Human Chikungunya Virus IgM (CHIKV IgM) Kit in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Chikungunya Virus IgM (CHIKV IgM) Kit |
KTE62973-96T |
Abbkine |
96T |
EUR 686.4 |
Description: Quantitative sandwich ELISA for measuring Human Chikungunya Virus IgM (CHIKV IgM) Kit in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
Chikungunya virus Non-structural polyprotein, |
1-CSB-EP810351CJAT |
Cusabio |
-
EUR 733.20
-
EUR 370.80
-
EUR 2192.40
-
EUR 1126.80
-
EUR 1461.60
-
EUR 476.40
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Chikungunya virus Non-structural polyprotein,Partial expressed in E.coli |
Chikungunya Virus (CHIKV) Polyclonal Antibody (Pan-species) |
4-PAX256Ge08 |
Cloud-Clone |
-
EUR 397.20
-
EUR 4596.00
-
EUR 1110.00
-
EUR 516.00
-
EUR 300.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pan-species Chikungunya Virus (CHIKV) |
Chikungunya Virus (CHIKV) Polyclonal Antibody (Pan-species), PE |
4-PAX256Ge08-PE |
Cloud-Clone |
-
EUR 476.40
-
EUR 4896.00
-
EUR 1332.00
-
EUR 619.20
-
EUR 285.60
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pan-species Chikungunya Virus (CHIKV). This antibody is labeled with PE. |
Chikungunya Virus (CHIKV) Polyclonal Antibody (Pan-species), APC |
4-PAX256Ge08-APC |
Cloud-Clone |
-
EUR 565.20
-
EUR 6090.00
-
EUR 1634.40
-
EUR 744.00
-
EUR 327.60
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pan-species Chikungunya Virus (CHIKV). This antibody is labeled with APC. |
Chikungunya Virus (CHIKV) Polyclonal Antibody (Pan-species), Cy3 |
4-PAX256Ge08-Cy3 |
Cloud-Clone |
-
EUR 704.40
-
EUR 8070.00
-
EUR 2130.00
-
EUR 942.00
-
EUR 387.60
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pan-species Chikungunya Virus (CHIKV). This antibody is labeled with Cy3. |
Chikungunya Virus (CHIKV) Polyclonal Antibody (Pan-species), HRP |
4-PAX256Ge08-HRP |
Cloud-Clone |
-
EUR 510.00
-
EUR 5298.00
-
EUR 1436.40
-
EUR 664.80
-
EUR 303.60
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pan-species Chikungunya Virus (CHIKV). This antibody is labeled with HRP. |
Chikungunya Virus (CHIKV) Polyclonal Antibody (Pan-species), FITC |
4-PAX256Ge08-FITC |
Cloud-Clone |
-
EUR 476.40
-
EUR 4896.00
-
EUR 1332.00
-
EUR 619.20
-
EUR 285.60
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pan-species Chikungunya Virus (CHIKV). This antibody is labeled with FITC. |
Recombinant Chikungunya Virus VLP Protein, Native, Human-100ug |
QP10473-100ug |
EnQuireBio |
100ug |
EUR 882 |
Recombinant Chikungunya Virus VLP Protein, Native, Human-500ug |
QP10473-500ug |
EnQuireBio |
500ug |
EUR 3145.2 |
Chikungunya Virus (CHIKV) Polyclonal Antibody (Pan-species), APC-Cy7 |
4-PAX256Ge08-APC-Cy7 |
Cloud-Clone |
-
EUR 987.60
-
EUR 12036.00
-
EUR 3126.00
-
EUR 1344.00
-
EUR 512.40
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pan-species Chikungunya Virus (CHIKV). This antibody is labeled with APC-Cy7. |
Recombinant Chikungunya Virus CHIKV E1 Protein, Untagged, E.coli-10ug |
QP11405-10ug |
EnQuireBio |
10ug |
EUR 241.2 |
Recombinant Chikungunya Virus CHIKV E1 Protein, Untagged, E.coli-2ug |
QP11405-2ug |
EnQuireBio |
2ug |
EUR 186 |
Recombinant Chikungunya Virus CHIKV E1 Protein, Untagged, E.coli-1mg |
QP11405-EC-1mg |
EnQuireBio |
1mg |
EUR 1513.2 |
Recombinant Chikungunya Virus CHIKV E2 Protein, Untagged, E.coli-1mg |
QP11406-1mg |
EnQuireBio |
1mg |
EUR 1513.2 |
Recombinant Chikungunya Virus CHIKV E1 Protein, Untagged, E.coli-100ug |
QP11405-100ug |
EnQuireBio |
100ug |
EUR 261.6 |
Recombinant Chikungunya Virus CHIKV E1 Protein, Untagged, E.coli-500ug |
QP11405-500ug |
EnQuireBio |
500ug |
EUR 795.6 |
Recombinant Chikungunya Virus CHIKV E1 Protein, Untagged, Insect-1mg |
QP11405-INSECT-1mg |
EnQuireBio |
1mg |
EUR 6301.2 |
Recombinant Chikungunya Virus CHIKV E2 Protein, Untagged, E.coli-100ug |
QP11406-100ug |
EnQuireBio |
100ug |
EUR 261.6 |
Recombinant Chikungunya Virus CHIKV E2 Protein, Untagged, E.coli-500ug |
QP11406-500ug |
EnQuireBio |
500ug |
EUR 795.6 |
Chikungunya Virus (CHIKV) Polyclonal Antibody (Pan-species), Biotinylated |
4-PAX256Ge08-Biotin |
Cloud-Clone |
-
EUR 484.80
-
EUR 4536.00
-
EUR 1268.40
-
EUR 615.60
-
EUR 309.60
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pan-species Chikungunya Virus (CHIKV). This antibody is labeled with Biotin. |
Recombinant Chikungunya Virus CHIKV Mutant Protein, Untagged, Insect-10ug |
QP11407-10ug |
EnQuireBio |
10ug |
EUR 241.2 |
Recombinant Chikungunya Virus CHIKV Mutant Protein, Untagged, Insect-1mg |
QP11407-1mg |
EnQuireBio |
1mg |
EUR 6301.2 |
Recombinant Chikungunya Virus CHIKV Mutant Protein, Untagged, Insect-2ug |
QP11407-2ug |
EnQuireBio |
2ug |
EUR 186 |
Recombinant Chikungunya Virus E1 Envelope Protein, Native, Human-100ug |
QP10474-100ug |
EnQuireBio |
100ug |
EUR 555.6 |
Recombinant Chikungunya Virus E1 Envelope Protein, Native, Human-500ug |
QP10474-500ug |
EnQuireBio |
500ug |
EUR 1904.4 |
Custom Testing of Samples for Antibodies (IgA/IgG/IgM) to Chikungunya virus (CHIKV) by ELISA |
530-400-CUX |
Alpha Diagnostics |
Custom |
Ask for price |
Recombinant (sf9) Chikungunya virus (CHIKV) E2 protein (>95%%, ~52 kda, his-tag) for ELISA/Western |
CHIKE26-R-10 |
Alpha Diagnostics |
10 ug |
EUR 416.4 |
Recombinant (E. coli) Chikungunya virus (CHIKV) E2 protein (>95%%, ~45 kda, his-tag) for ELISA/Western |
CHIKE25-R-10 |
Alpha Diagnostics |
10 ug |
EUR 416.4 |
Recombinant (E. coli) Chikungunya virus (CHIKV) capsid protein (>95%%, ~35 kda, his-tag) for ELISA/Western |
CHIKC35-R-10 |
Alpha Diagnostics |
10 ug |
EUR 416.4 |
Monkeypox Virus Real Time PCR Kit |
PDPS-AR064 |
Creative Biogene |
1 unit |
Ask for price |
Description: Creative Biogene Monkeypox Virus Real Time PCR Kit is used for the detection of monkeypox Virus in serum or lesion exudate samples by using real time PCR systems. Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae. It is one of the human orthopoxviruses that includes variola (VARV), cowpox (CPX), and vaccinia (VACV) viruses. The kit contains a specific ready-to-use system for the detection of the monkeypox Virus. Fluorescence is emitted and measured by the real time systems' optical unit during the PCR. |
Monkeypox Virus Real Time PCR Kit |
ZD-0076-01 |
Gentaur Genprice |
25 tests/kit |
Ask for price |
|
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load. |
Monkeypox Virus Real Time PCR Kit |
ZD-0076-02 |
Gentaur Genprice |
25 tests/kit |
Ask for price |
|
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load. |
Monkeypox Virus Real Time PCR Kit |
YJC70115NW-25T |
Jiangsu Bioperfectus Technologies |
25 tests/kit |
Ask for price |
Description: The Bioperfectus Monkeypox Virus Real Time PCR Kit is an in vitro diagnostic test, based on real-time PCR technology, for the detection of DNA from the Monkeypox virus. Specimens can be obtained from human serum, lesion exudate samples and scab. BSL-2 facilities with standard BSL-2 work practices may be used for the test of t he Monkeypox virus. |
Chikungunya E2 Protein |
abx160009-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Recombinant (sf9) Chikungunya virus (CHIKV) E1 protein (Wild-type, ectodomain, 415-aa>95%, his-tag) for ELISA/Western |
CHIKE15-R-10 |
Alpha Diagnostics |
10 ug |
EUR 416.4 |
Chikungunya Capsid Protein |
abx061511-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Chikungunya Capsid Protein |
abx061512-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Recombinant (sf9) Chikungunya virus (CHIKV) E1 protein (A226V Mutant ectodomain, 415aa, >95%%, his-tag) for ELISA/Western |
CHIKE16-R-10 |
Alpha Diagnostics |
10 ug |
EUR 416.4 |
Chikungunya E1 Mutant Protein |
abx160006-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Chikungunya Wild Type E1 Protein |
20-abx262345 |
Abbexa |
-
EUR 393.60
-
EUR 7676.40
-
EUR 276.00
|
|
|
Chikungunya E1 Wild-Type Protein |
abx160007-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Chikungunya Mutant (A226V) E1 Protein |
20-abx262346 |
Abbexa |
-
EUR 393.60
-
EUR 7676.40
-
EUR 276.00
|
|
|
Chikungunya E1-E2 Wild Type Protein |
abx160008-100ug |
Abbexa |
100 ug |
EUR 661.2 |
|
NATtrol Chikungunya Stock (Qualitative) (1 mL) |
NATCHIKV-ST |
Zeptometrix |
1.0mL |
EUR 1281 |
Monoclonal Anti-Zika Virus Envelope Protein (African) IgG Clone #1 (specific for Zika non-reactive with WNV, JEV, Chikungunya) |
ZENV12-M |
Alpha Diagnostics |
100 ul |
EUR 578.4 |
Monoclonal Anti-Zika Virus Envelope Protein (African) IgG Clone #2 ((specific for Zika non-reactive with WNV, JEV, Chikungunya) |
ZENV13-M |
Alpha Diagnostics |
100 ul |
EUR 578.4 |
CHIKV Chikungunya Mutant (A226V) E1 Recombinant Protein |
PROTG0LWX6 |
BosterBio |
Regular: 10ug |
EUR 380.4 |
Description: Recombinant Chikungunya Mutant (A226V) E1 produced in Insect Cells is a polypeptide chain containing amino acids 1-415, however at position 226 the Alanine of the wild-type CHIKV E1 gene was mutated to Valine. The molecular weight of the CHIKV Mutant is approximately 50kDa. The E1 protein is C-terminal part of E2-6K-E1 protein region. CHIKV Mutant is purified by proprietary chromatographic technique. |
Monoclonal Anti-Zika Virus Envelope domain III Protein (African) IgG1 Clone #1 (specific for Zika non-reactive with WNV, JEV, Chikungunya) |
ZEND21-M |
Alpha Diagnostics |
100 ul |
EUR 578.4 |
0.2ML CLEAR STRIP CAPS FOR REAL TIME PCR |
PCR-2CP-RT-C |
CORNING |
125/pk |
EUR 267.6 |
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit |
RR-0478-02 |
Liferiver |
25 tests/kit |
EUR 1189.2 |
|
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. |
AzuraQuant cDNA Synthesis Kit - 100 Reactions |
AZ-1996 |
Real Time Primers |
100 Reactions |
EUR 446.4 |
Eztime Real - time PCRPremix 2x,Taqman |
FYT105-100P |
Yeastern Biotech |
100 Preps 1.25 ml |
Ask for price |
AzuraQuant cDNA Synthesis Kit - 25 Reactions |
AZ-1995 |
Real Time Primers |
25 Reactions |
EUR 183.6 |
Eztime Real - time PCRPremix 2x,SYBR Green |
FYT103-100P |
Yeastern Biotech |
100 Preps 1.25 ml |
Ask for price |
Eztime Real - time PCRPremix 2x,SYBR Green |
FYT103-400P |
Yeastern Biotech |
400 Preps 1.25 ml x 4 |
Ask for price |
Eztime Real - time PCRPremix 2x,Taqman, ROX |
FYT105-400P |
Yeastern Biotech |
400 Preps 1.25 ml x 4 |
Ask for price |
Eztime Real - time PCRPremix 2x,Taqman, ROX |
FYT106-100P |
Yeastern Biotech |
100 Preps 1.25 ml |
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Eztime Real - time PCRPremix 2x,SYBRGreen , ROX |
FYT104-100P |
Yeastern Biotech |
100 Preps 1.25 ml |
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Eztime Real - time PCRPremix 2x,SYBRGreen , ROX |
FYT104-400P |
Yeastern Biotech |
400 Preps 1.25 ml x 4 |
Ask for price |
Chicken anemia virus RT PCR kit |
RTq-V003-100D |
Bioingentech |
100T |
EUR 860.4 |
Chicken anemia virus RT PCR kit |
RTq-V003-150D |
Bioingentech |
150T |
EUR 969.6 |
Chicken anemia virus RT PCR kit |
RTq-V003-50D |
Bioingentech |
50T |
EUR 717.6 |
K Virus RT PCR kit |
RTq-V365-100D |
Bioingentech |
100T |
EUR 860.4 |
K Virus RT PCR kit |
RTq-V365-150D |
Bioingentech |
150T |
EUR 969.6 |
Virus Zika RT PCR kit |
RTq-H434-100R |
Bioingentech |
100T |
EUR 1573.2 |
Virus Zika RT PCR kit |
RTq-H434-150R |
Bioingentech |
150T |
EUR 2144.4 |
Virus Zika RT PCR kit |
RTq-H434-50R |
Bioingentech |
50T |
EUR 1155.6 |
Mumps Virus RT PCR kit |
RTq-H640-100R |
Bioingentech |
100T |
EUR 1573.2 |
Mumps Virus RT PCR kit |
RTq-H640-150R |
Bioingentech |
150T |
EUR 2144.4 |
Mumps Virus RT PCR kit |
RTq-H640-50R |
Bioingentech |
50T |
EUR 1155.6 |
Irido virus RT PCR kit |
RTq-V288-100D |
Bioingentech |
100T |
EUR 860.4 |
Irido virus RT PCR kit |
RTq-V288-150D |
Bioingentech |
150T |
EUR 969.6 |
Irido virus RT PCR kit |
RTq-V288-50D |
Bioingentech |
50T |
EUR 717.6 |
Junin Virus RT PCR kit |
RTq-V388-100R |
Bioingentech |
100T |
EUR 1573.2 |
Junin Virus RT PCR kit |
RTq-V388-150R |
Bioingentech |
150T |
EUR 2144.4 |
Junin Virus RT PCR kit |
RTq-V388-50R |
Bioingentech |
50T |
EUR 1155.6 |
Lassa virus RT PCR kit |
RTq-V390-100R |
Bioingentech |
100T |
EUR 1573.2 |
Lassa virus RT PCR kit |
RTq-V390-150R |
Bioingentech |
150T |
EUR 2144.4 |
Lassa virus RT PCR kit |
RTq-V390-50R |
Bioingentech |
50T |
EUR 1155.6 |
Sabia Virus RT PCR kit |
RTq-V392-100R |
Bioingentech |
100T |
EUR 1573.2 |
Sabia Virus RT PCR kit |
RTq-V392-150R |
Bioingentech |
150T |
EUR 2144.4 |
Sabia Virus RT PCR kit |
RTq-V392-50R |
Bioingentech |
50T |
EUR 1155.6 |
Visna virus RT PCR kit |
RTq-V634-100R |
Bioingentech |
100T |
EUR 1573.2 |
Visna virus RT PCR kit |
RTq-V634-150R |
Bioingentech |
150T |
EUR 2144.4 |
Visna virus RT PCR kit |
RTq-V634-50R |
Bioingentech |
50T |
EUR 1155.6 |
Nipah virus RT PCR kit |
RTq-VH139-100R |
Bioingentech |
100T |
EUR 1573.2 |
Nipah virus RT PCR kit |
RTq-VH139-150R |
Bioingentech |
150T |
EUR 2144.4 |
On this examine, particular person exosomes had been noticed on a trajectory when passing by the laser beam sheet and had been counted for 10 min at a relentless circulate velocity. The entire evaluation time was lower than 1.5 h together with the steps required to take away massive particles and permit response with the antibody. The outcomes for 2 samples ready with and with out the isolation of exosomes confirmed a lack of exosomes within the isolation step. We additionally decided the variety of the exosomes secreted by the cells to a tradition medium throughout cultivation. As anticipated, the overall variety of exosomes in a tradition medium elevated with a rise within the cultivation time, and the variety of exosomes launched each 12 h both remained fixed or confirmed not more than a slight improve for so long as 72 h. It was unclear whether or not the quantity exosomes was depending on the cell inhabitants at confluences of 10-60%.