Lipid Peroxidation (MDA + HNE) Assay Package
Lipid peroxidation is a widely known instance of oxidative harm in cell membranes, lipoproteins, and different lipid-containing buildings. Peroxidative modification of unsaturated phospholipids, glycolipids, and ldl cholesterol can happen in several reactions. They are often triggered by i) free radical species corresponding to oxyl radicals, peroxyl radicals, and hydroxyl radicals derived from iron-mediated discount of hydrogen peroxide or ii) non-radical species corresponding to singlet oxygen, ozone, and peroxynitrite generated by the response of superoxide with nitric oxide.
Malondialdehyde (MDA) and 4-hydroxyalkenals are vital poisonous byproducts of lipid peroxidation. So, the measurement of the quantities of such aldehydes corresponds to an index of lipid peroxidation in vitro and in vivo. 4-Hydroxynonenal (4-HNE) is a serious product of the peroxidative decomposition of ω-6 polyunsaturated fatty acids (PUFA). It possesses cytotoxic, hepatotoxic, mutagenic, and genotoxic properties. Moreover, elevated ranges of HNE had been present in plasma and varied organs beneath oxidative stress circumstances. The truth is, MDA is in lots of situations probably the most ample particular person aldehyde ensuing from lipid peroxidation. In vitro MDA can alter proteins, DNA, RNA, and plenty of different biomolecules.
Bioquochem’s LPO assay equipment measures MDA and HNE concentrations as an index of lipid peroxidation. Firstly, acid-catalyzed assault on the 3-position of the indole ring initiates the reactions between indoles and aldehydes (MDA and HNE). Because of this, this response provides a diindolylalkane (chromophore) with most absorbance within the area of 580-620 nm.
In our assay an indol (Reagent A) reacts rapidly with MDA and HNE in acidic medium, yielding a chromophore (C) with a excessive molar extinction coefficient at its maximal absorption wavelength of 586 nm.

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Product overview
Lipid Peroxidation (MDA) Assay Package (Colorimetric/Fluorometric) (ab118970) supplies a handy software for delicate detection of malondialdehyde (MDA).
Within the lipid peroxidation assay protocol, the MDA within the pattern reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct could be simply quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA ranges as little as 1 nmol/effectively colorimetrically and 0.1 nmol/effectively fluorometrically.
The MDA assay can also be refered to as a TBARS assay.
Lipid peroxidation assay protocol abstract:
– add TBA answer to samples and requirements, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min
– switch to wells of microplate
– analyze with microplate reader
For greater sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet earlier than evaluation.Chinese language protocol out there. See protocols part under.
For an alternate MDA assay, with out the heating steps required within the TBARS assay, attempt MDA assay ab233471.
Lipid Peroxidation biovision -
Notes
Lipid peroxidation refers back to the oxidative degradation of lipids. On this course of free radicals take electrons from the lipids (typically in cell membranes), leading to cell harm. Quantification of lipid peroxidation is crucial to evaluate oxidative stress. Lipid peroxidation varieties reactive aldehydes corresponding to malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as pure bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are sometimes used as markers of lipid peroxidation, and to assay for oxidative harm / oxidative stress.
Associated merchandise
Assessment the oxidative stress marker and assay information, or the total metabolism assay information to study extra assays for metabolites, metabolic enzymes, mitochondrial operate, and oxidative stress, and in addition how one can assay metabolic operate in dwell cells utilizing your plate reader.
Additionally see the favored 4-HNE Assay Package ab238538 as a substitute marker of lipid peroxidation and oxidative stress.
How different researchers have used Lipid Peroxidation Assay Package ab118970The MDA/TBARs assay equipment has been utilized in publications in a wide range of pattern sorts, together with:
– Human: serum1, hippocampal main cell extracts2, A375 cultured cell lysates3, plasma and platelet samples4
– Mouse: neuronal cell lysates5, coronary heart tissue extract6, plasma7, cell extracts8
– Rat: hippocampal tissue extracts9, cardiomyocyte extracts of cultured cells10, lung lysates11
– Pig: serum12References: 1 – Shen J et al. 2018, 2 – Wang Q et al. 2019, 3 – Luo M et al. 2018, 4 – Mustafa AG et al. 2018, 5 – Murphy Okay et al. 2018, 6 – Guan F et al. 2019, 7 – Costa CRC et al. 2018, 8 – Eleftheriadis T et al. 2019, 9 – Malekiyan et al. 2019, 10 – Zhou Z et al. 2018, 11 – Li L et al. 2018, 12 – Lee SE and Kang KS 2019
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Platform
Microplate reader
U-74389G (Lipid peroxidation blocker) |
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SIH-205-100MG | Stressmarq | 100 mg | EUR 208.8 |
Description: The substance U-74389G is a lipid peroxidation blocker. It is synthetically produced and has a purity of >99%. The pure substance is white solid which is soluble in 25 mg/ml DMSO, and in 20 mg/ml in ethanol; Insoluble in water. |
U-74389G (Lipid peroxidation blocker) |
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SIH-205-500MG | Stressmarq | 500 mg | EUR 698.4 |
Description: The substance U-74389G is a lipid peroxidation blocker. It is synthetically produced and has a purity of >99%. The pure substance is white solid which is soluble in 25 mg/ml DMSO, and in 20 mg/ml in ethanol; Insoluble in water. |
Lipid Peroxidation Colorimetric Assay Kit |
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K454-100 | Biovision | each | EUR 652.8 |
Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit |
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K2167-100 | ApexBio | 100 assays | EUR 703.2 |
Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit |
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K739-100 | Biovision | each | EUR 698.4 |
Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit |
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22906 | AAT Bioquest | 200 Tests | EUR 367.2 |
Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit |
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15991 | AAT Bioquest | 200 tests | EUR 471.6 |
Myeloperoxidase (MPO) Peroxidation Activity Assay Kit |
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K747-100 | Biovision | each | EUR 392.4 |
OxiSelect Myeloperoxidase Peroxidation Activity Assay Kit (Fluorometric) |
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STA-805 | Cell Biolabs | 192 assays | EUR 692.4 |
Description: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity. The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, fluorometric assay for measuring the myeloperoxidase peroxidation activity within a sample. |
Lipid A Lipopolysaccharide (LPS Lipid A / Endotoxin) Antibody |
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abx415762-1ml | Abbexa | 1 ml | EUR 727.2 |
Lipid A Antibody |
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abx023083-1ml | Abbexa | 1 ml | EUR 886.8 |
Lipid Extraction & Polar/Neutral Lipid Separation Combo Kit (Chloroform Free) |
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MET-5009-C | Cell Biolabs | 50 preps | EUR 790.8 |
Description: This kit combines a chloroform free organic lipid extraction with further separation of polar and neutral lipids. The lipid extraction first extracts lipids from a crude lipid source to a chloroform free upper organic phase using a proprietary alcohol. After addition of a proprietary organic compound, the mixture is centrifuged to separate the phases. The recovered organic phase containing lipids is dried and then polar and neutral lipids are separated by adding a proprietary secondary alcohol and organic compound. The layers are separated, dried, and resuspended for further analysis. |
Ceraplex Lipid Concentrate(1000X) |
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C3316-010 | GenDepot | 100ml | EUR 144 |
Ceraplex Lipid Concentrate(1000X) |
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C3316-050 | GenDepot | 500ml | EUR 295.2 |
Lipid Droplet Isolation Kit |
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MET-5011 | Cell Biolabs | 50 preps | EUR 498 |
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer. |
Lipid Quantitation Kit (Colorimetric) |
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STA-613 | Cell Biolabs | 100 assays | EUR 637.2 |
Description: Cell Biolabs? Lipid Quantification Kit measures the lipid content (unsaturated fatty acids only) of samples using the sulfo-phospho-vanillin method, resulting in a simple colorimetric readout amenable to multi-well plate detection. |