Lipid Peroxidation

Lipid Peroxidation (MDA) Assay Kit

Lipid Peroxidation (MDA + HNE) Assay Package

Lipid peroxidation is a widely known instance of oxidative harm in cell membranes, lipoproteins, and different lipid-containing buildings. Peroxidative modification of unsaturated phospholipids, glycolipids, and ldl cholesterol can happen in several reactions. They are often triggered by i) free radical species corresponding to oxyl radicals, peroxyl radicals, and hydroxyl radicals derived from iron-mediated discount of hydrogen peroxide or ii) non-radical species corresponding to singlet oxygen, ozone, and peroxynitrite generated by the response of superoxide with nitric oxide.

Malondialdehyde (MDA) and 4-hydroxyalkenals are vital poisonous byproducts of lipid peroxidation. So, the measurement of the quantities of such aldehydes corresponds to an index of lipid peroxidation in vitro and in vivo. 4-Hydroxynonenal (4-HNE) is a serious product of the peroxidative decomposition of ω-6 polyunsaturated fatty acids (PUFA). It possesses cytotoxic, hepatotoxic, mutagenic, and genotoxic properties. Moreover, elevated ranges of HNE had been present in plasma and varied organs beneath oxidative stress circumstances. The truth is, MDA is in lots of situations probably the most ample particular person aldehyde ensuing from lipid peroxidation. In vitro MDA can alter proteins, DNA, RNA, and plenty of different biomolecules.

Bioquochem’s LPO assay equipment measures MDA and HNE concentrations as an index of lipid peroxidation. Firstly, acid-catalyzed assault on the 3-position of the indole ring initiates the reactions between indoles and aldehydes (MDA and HNE). Because of this, this response provides a diindolylalkane (chromophore) with most absorbance within the area of 580-620 nm.

In our assay an indol (Reagent A) reacts rapidly with MDA and HNE in acidic medium, yielding a chromophore (C) with a excessive molar extinction coefficient at its maximal absorption wavelength of 586 nm.

  • Product overview

    Lipid Peroxidation (MDA) Assay Package (Colorimetric/Fluorometric) (ab118970) supplies a handy software for delicate detection of malondialdehyde (MDA).

    Within the lipid peroxidation assay protocol, the MDA within the pattern reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct could be simply quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA ranges as little as 1 nmol/effectively colorimetrically and 0.1 nmol/effectively fluorometrically.

    The MDA assay can also be refered to as a TBARS assay.

    Lipid peroxidation assay protocol abstract:
    – add TBA answer to samples and requirements, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min
    – switch to wells of microplate
    – analyze with microplate reader
    For greater sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet earlier than evaluation.

    Chinese language protocol out there. See protocols part under.

    For an alternate MDA assay, with out the heating steps required within the TBARS assay, attempt MDA assay ab233471.

    Lipid Peroxidation biovision
    Lipid Peroxidation biovision
  • Notes

    Lipid peroxidation refers back to the oxidative degradation of lipids. On this course of free radicals take electrons from the lipids (typically in cell membranes), leading to cell harm. Quantification of lipid peroxidation is crucial to evaluate oxidative stress. Lipid peroxidation varieties reactive aldehydes corresponding to malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as pure bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are sometimes used as markers of lipid peroxidation, and to assay for oxidative harm / oxidative stress.

    Associated merchandise

    Assessment the oxidative stress marker and assay information, or the total metabolism assay information to study extra assays for metabolites, metabolic enzymes, mitochondrial operate, and oxidative stress, and in addition how one can assay metabolic operate in dwell cells utilizing your plate reader.

    Additionally see the favored 4-HNE Assay Package ab238538 as a substitute marker of lipid peroxidation and oxidative stress.

    How different researchers have used Lipid Peroxidation Assay Package ab118970

    The MDA/TBARs assay equipment has been utilized in publications in a wide range of pattern sorts, together with:
    – Human: serum1, hippocampal main cell extracts2, A375 cultured cell lysates3, plasma and platelet samples4
    – Mouse: neuronal cell lysates5, coronary heart tissue extract6, plasma7, cell extracts8
    – Rat: hippocampal tissue extracts9, cardiomyocyte extracts of cultured cells10, lung lysates11
    – Pig: serum12

    References: 1 – Shen J et al. 2018, 2 – Wang Q et al. 2019, 3 – Luo M et al. 2018,  4 – Mustafa AG et al. 2018, 5 – Murphy Okay et al. 2018, 6 – Guan F et al. 2019, 7 – Costa CRC et al. 2018, 8 – Eleftheriadis T et al. 2019, 9 – Malekiyan et al. 2019, 10 – Zhou Z et al. 2018, 11 – Li L et al. 2018, 12 – Lee SE and Kang KS 2019

  • Platform

    Microplate reader

Lipid peroxidation Assay Kit

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Description: Lipid peroxidation inhibitor 1

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Description: Lipid peroxidation inhibitor 1

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Lipid Peroxidation (LPO) Assay Kit

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EUR 425

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