abm’s qPCR Retrovirus Titer Equipment is a one-step assay by qPCR which doesn’t require extra lysis or reverse transcription (RT) steps, and will be accomplished below one hour. Designed to ship excessive sensitivity and specificity, the equipment ensures minimal non-specific background and higher total efficiency in comparison with comparable kits in the marketplace. ROX reference dye is offered separate from the MasterMix, making this equipment universally suitable with most qPCR devices.
PRODUCT COMPONENT
QUANTITY
BlasTaq 2X qPCR Titer MasterMix
1.25 ml
Primer Combine
100 rxn (200 µl)
Commonplace Management DNA
50 µl
ROX Reference Dye
15 µl
Nuclease-Free H2O
2 x 1.zero ml
SKU
G949
Unit amount
100 rxn
Storage Situation
Retailer at -20°C
qPCR and One-Step RT MasterMixes
Virus Titration Kits
Our Titration Kits present a quick and easy technique for titrating your virus. The kits make use of both a fast RNA or DNA extraction step, and decide viral RNA/DNA titer utilizing qRT-PCR.
Benefits of abm’s titration kits:
No RNA purification — saves time and eliminates inaccuracy.
qRT-PCR in a single step — extra delicate and correct than different strategies.
No NTC amplification — Distinctive primer design utterly eliminates non-specific amplification in NTCs.
We additionally supply On the spot Lentivirus Titration Playing cards, which permit fast detection of lentiviral capsule proteins as a marker to substantiate the presence of lentivirus. Using the lateral circulate immunoassay technique, viral titer yields are assessed immediately inside 10 minutes utilizing our three step protocol.
he Retro-X qRT-PCR Titration Equipment gives a quick and easy technique for measuring retrovirus titer. The kits use a fast RNA purification step earlier than quantifying the variety of retroviral genome copies utilizing qRT-PCR and TB Inexperienced applied sciences.
Fast willpower of retroviral titer
Whereas conventional titration strategies require as much as 10 days to finish, these titration kits require solely four hours and work with all MMLV-based retroviral vectors (not MSCV). Utilizing qRT-PCR dramatically shortens the time interval between viral harvest and target-cell an infection, permitting you to carry out each on the identical day.
Obtain constant retroviral transductions
A serious benefit of retroviral gene-delivery techniques is the power to regulate the extent of gene expression by adjusting the multiplicity of an infection (MOI) to dictate the variety of viral genomes that enter your goal cells. These titration kits let you make a fast correlation between the retroviral copy quantity and retroviral infectivity in your cell sort and in the end let you management the expression stage between experiments.
The way it works
The qRT-PCR process entails subjecting serial dilutions of your pattern and a management RNA of recognized copy quantity (offered) to one-step qRT-PCR. The retroviral copy quantity contained in your pattern can then be decided by evaluating its Ct worth to the usual curve.
RNA purification and DNase I therapy steps are crucial to the protocol so even viruses packaged utilizing transient transfections, which at all times include residual plasmid DNA, will be precisely quantified utilizing this equipment.
