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Proteinase K, recombinant

 

Proteinase Okay – one of many typically used enzymes in molecular biology.

Glorious different for protein digestion in assorted features.

  • BLIRT – skilled in Proteinase Okay manufacturing for over 15 years.
  • Licensed manufacturing course of ISO 13485 ensuing throughout the highest prime quality product with a very low batch-to-batch variability.
  • Huge parts, the chance of white labeling, and portioning service – liquid variety throughout the amount on the client’s request (as a lot as a variety of million samples month-to-month).

 

Thermo Scientific Proteinase Okay is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even throughout the presence of detergents. Proteinase Okay cleaves peptide bonds on the carboxylic sides of aliphatic, aromatic, or hydrophobic amino acids. The Proteinase Okay is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

Proteinase Okay (Lyophilized)

For Fundamental Digestion of Protein in Natural Samples

  • Full of life over a pH differ of 4.3–12.0, in 0.5% SDS or 1% Triton® X-100
  • Purified to remove RNase and DNase actions

Safe at Room Temperature and Easy to Use

  • Full of life over pH differ 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
  • Retains >80% train at temperatures as a lot as 60°C
  • No resuspension or thawing sooner than use
  • Cat.# MC5005, MC5008 supplied at a spotlight of 20mg/ml



Highlights

• Ready-to-use reply
• Full of life in a wide range of response circumstances

Capabilities

• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces
• Dedication of enzyme localization
• Enhancing cloning effectivity of PCR merchandise

Remember

• The actually helpful working focus of Proteinase Okay is 0.05 to 1 mg/mL. The train of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to 4 M urea
• Ca2+ protects Proteinase Okay in direction of autolysis, will improve the thermal stability, and has a regulatory carry out for the substrate binding site of Proteinase Okay
• Safe over a big pH differ: 4.Zero to 12.5, optimum pH 7.5 to eight.0
• Optimum train at 50 to55°C
• Speedy denaturation of enzyme occurs at temperatures above 65°C.

 

Proteinase Okay Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It is a broad-spectrum endopeptidase with a very extreme specific train.

Recombinant Proteinase Okay enzyme is expressed in Pichia pastoris, and undergoes in depth purification to yield the easiest prime quality product.

Proteinase Okay Molecular Biology Grade (PCR Grade) is full of life beneath a wide range of response circumstances, along with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively using for the digestion of proteins, along with DNases and RNases, all through nucleic acid preparations with out compromising the integrity of isolated DNA or RNA.

Proteinase K, recombinant
Proteinase Ok, recombinant

Choices

  • Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
  • Extreme train and distinctive purity.
  • Full of life at extreme temperatures (as a lot as 56 °C) and denaturing circumstances (e.g. throughout the presence of urea and/or SDS), which makes it easiest for digesting proteins in a variety of features.
  • Safe over a big pH differ: 4.0–12.5 (optimum pH 7.5–8.0).
  • Decreased amount of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
  • Accessible as powder, lyophilized “cake” or liquid.

Capabilities:

  • Extraction of DNA and RNA from completely totally different starting provides.
  • Purification of objective supplies from contaminating proteins.
  • Elimination of DNases and RNases all through nucleic acids isolation.

 

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