ATpase assay

Key Options:

Non-radioactive, homogeneous assays
• Particular assays
• Handy: minimal pattern preparation; quick protocols (1-2 hours )
• Value efficient: 100 assays;
• Excessive Throughput Screening appropriate
• Validated: utilizing mammalian tissues, cells, organic fluids

BioVision affords complete in vitro bioassays which are utilized by hundreds of worldwide scientists in quite a few illness associated areas like most cancers, diabetes, weight problems, heart problems, liver illness, kidney illness, Alzheimer’s illness and infectious illnesses, and so on., in addition to different analysis areas equivalent to metabolism, neuroscience, epigenetics, oxidative stress, stem cells, protein extraction and pattern preparation, and so on. Our high-quality bioassays supply specificity, sensitivity, reproducibility and but reasonably priced. Our mission is to ship revolutionary bioassays primarily based on leading edge analysis to fulfill your scientific wants. BioVision’s bioassay kits can be utilized with a few of the hottest and customary analysis instrumentation (i.e. microplate reader, movement cytometer, and microscopes).

 

Inorganic ions play quite a few physiological roles in nature. Cations and anions take part in neurological transmission, muscle contraction, intra-cellular water transport, acid-base stability in blood, bone formation, vitality switch and storage. Moreover, they perform as cofactors or may be discovered as moieties inside
different essential biomolecules. BioVision has a terrific portfolio of assays measuring inorganic ions together with assays for sodium, calcium, phosphate and plenty of others.

BioVision’s intensive microplate-based assay portfolio affords quite a few merchandise that can be utilized for the detection/quantification of enzymatic exercise, quantification of particular chemical analytes in organic samples and for
high-throughput inhibitor screening. Our merchandise make the most of mixtures of proprietary enzymes and chemical probes, fastidiously engineered and characterised for the detection of related targets. Our kits have been examined and validated utilizing actual organic samples (equivalent to blood, serum, plasma, urine, saliva, cell and tissue lysates and foodstuffs), making certain that analytes are detectable in real-world organic matrices.

Pattern utility is maybe an important issue when selecting the right product to your analysis wants. This may decide the mandatory assay sensitivity and subsequently the popular detection technique. Our hottest merchandise make the most of secure chromophores and fluorophores that present sturdy alerts over time. PicoProbe, our proprietary fluorophore, is without doubt one of the most delicate probes available in the market. It will possibly detect as little as 20 pmoles of analyte per analyzed pattern properly.

 

Metabolism performs a key function in varied areas equivalent to physiology, cell biology, and drugs. Analysis in metabolism is dramatically elevated by the conclusion that many frequent human illnesses equivalent to most cancers, diabetes, weight problems, hypoglycemia, hypolipidemia, phenylketonuria, neurodegeneration, and so on. are resulting from perturbations in metabolic pathways. Understanding the metabolic variations and attainable outliers is thus an important facet of disease-oriented analysis. Then again, reactive oxygen species (ROS), produced endogenously on account of regular mobile metabolic capabilities or from exterior sources, poses a major menace to cells since publicity to ROS may end up in severedamage to DNA, protein, and lipids. Figuring out particular types of oxidative harm is commonly the point of interest of curiosity  in several types of cancers and varied neurological illnesses. Antioxidant defenses to reduce fluctuations in ROS are important to cell survival and dictate approaches to illness remedies to cut back threat of most cancers or age-related purposeful deterioration.

 

Nonetheless, organic processes are higher characterised by finding out mobile interplay resulting from exogenous elements. Preclinical and Scientific Analysis depend on assays that examine totally different facets of mobile conduct (development by means of the cell cycle, migration, invasion) and measure the content material of key constructing blocks within the cell are generally used to observe the well being of cells in tradition and their response to varied stimuli (migration inducers, cell cycle arrest medicine, ldl cholesterol transport inhibitors and so on.).
BioVision is proud to supply the broadest Metabolism Assay portfolio starting from the detection of enzymatic exercise, metabolites equivalent to carbohydrates, lipids, amino acids, inorganic ions, serum parts, coenzymes & cofactors to oxidative stress, glycolysis pathway, and middleman metabolism. Additionally, we provide a decent collection of assay kits, antioxidants, inhibitors/activators of key ROS gamers and a increasing collection of assays to check some elementary processes governing mobile biology.

 

ATPase Activity Assay Kit (Colorimetric)
K417-100	Biovision	each	EUR 516

QuantiChrom™ ATPase/GTPase Assay Kit
DATG-200	BioAssay Systems	200	EUR 389
Description: For quantitative determination of ATPase or GTPase activity and high-throughput screen for their inhibitors. Key Features: High sensitivity. Detection of 0.007 U/L ATPase or GTPase activity. Fast and convenient: single reagent, homogeneous "mix-and-measure" assay allows quantitation of enzyme activity within 30 minutes. Robust and amenable to HTS: detection at 620nm greatly reduces potential interference by colored compounds. Z factors of >0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: OD620nm. Samples: ATPase and GTPase. Species: All. Procedure: Assay takes 30 min. Kit size: 200 tests. Detection limit: 0.007 U/L.

E. coli DNA Helicase ATPase assay Kit Plus-100
DNAB100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x E. coli DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

E. coli DNA Helicase ATPase assay Kit Plus-500
DNAB500KE	ProFoldin	500 assays	EUR 1396.74
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x E. coli DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. aureus DNA Helicase ATPase assay Kit Plus-100
DNAB100KS	ProFoldin	100 assays	EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x S. aureus DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

S. aureus DNA Helicase ATPase assay Kit Plus-500
DNAB500KS	ProFoldin	500 assays	EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x S. aureus DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

E. coli gyrase ATPase assay Kit Plus (enzyme included)
T2A-100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes the reaction buffer, DNA, E. coli gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.

H. influenzae DNA Helicase ATPase assay Kit Plus-100
DNAB100KH	ProFoldin	100 assays	EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x H. influenzae DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

S. pneumoniae DNA Helicase ATPase assay Kit Plus-100
DNAB100KN	ProFoldin	100 assays	EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x S. pneumoniae DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

P. aeruginosa DNA Helicase ATPase assay Kit Plus-100
DNAB100KP	ProFoldin	100 assays	EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x P. aeruginosa DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

H. influenzae DNA Helicase ATPase assay Kit Plus-500
DNAB500KH	ProFoldin	500 assays	EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x H. influenzae DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. pneumoniae DNA Helicase ATPase assay Kit Plus-500
DNAB500KN	ProFoldin	500 assays	EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x S. pneumoniae DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

P. aeruginosa DNA Helicase ATPase assay Kit Plus-500
DNAB500KP	ProFoldin	500 assays	EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x P. aeruginosa DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. aureus gyrase ATPase assay Kit Plus (enzyme included)
T2A-100KS	ProFoldin	100 assays	EUR 423.72
Description: This product includes the reaction buffer, DNA, S. aureus gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.

E. coli Topo IV ATPase Assay Kit Plus (enzyme included)
T4A-100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes 400 ul of 10 x Buffer, 35 ul of 100 x DNA, 33 ul of 100 x E. coli Topo IV, 35 ul of 100 x ATP and 5 ml of Dye for 100 assays of E. coli Topo IV ATPase activity in a 384-well assay format.

P. aeruginosa Gyrase ATPase assay Kit Plus (enzyme included)
T2A-100KP	ProFoldin	100 assays	EUR 444.91
Description: This product includes the reaction buffer, DNA, P. aeruginosa gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.

S. pneumoniae Topo IV ATPase Assay Kit Plus (enzyme included)
T4A-100KN	ProFoldin	100 assays	EUR 444.91
Description: This product includes 400 ul of 10 x Buffer, 35 ul of 100 x DNA, 33 ul of 100 x S. pneumoniae Topo IV, 35 ul of 100 x ATP and 5 ml of Dye for 100 assays of S. pneumoniae Topo IV ATPase activity in a 384-well assay format.

S. aureus Topoisomerase IV ATPase Assay Kit Plus (enzyme included)
T4A-100KS	ProFoldin	100 assays	EUR 444.91
Description: This product includes 400 ul of 10 x Buffer, 35 ul of 100 x DNA, 33 ul of 100 x S. aureus Topo IV, 35 ul of 100 x ATP and 5 ml of Dye for 100 assays of S. aureus Topo IV ATPase activity in a 384-well assay format.

P. aeruginosa Topoisomerase IV ATPase Assay Kit Plus (enzyme included)
T4A-100KP	ProFoldin	100 assays	EUR 444.91
Description: This product includes 400 ul of 10 x Buffer, 35 ul of 100 x DNA, 33 ul of 100 x P. aeruginosa Topo IV, 35 ul of 100 x ATP and 5 ml of Dye for 100 assays of P. aeruginosa Topo IV ATPase activity in a 384-well assay format.

Potassium-Transporting ATPase (H/K-ATPase) AssayLite Antibody (RPE Conjugate)
33239-05151	AssayPro	150 ug	EUR 513.6

Potassium-Transporting ATPase (H/K-ATPase) AssayLite Antibody (APC Conjugate)
33239-05161	AssayPro	150 ug	EUR 513.6

Potassium-Transporting ATPase (H/K-ATPase) AssayLite Antibody (FITC Conjugate)
33239-05141	AssayPro	150 ug	EUR 513.6

Potassium-Transporting ATPase (H/K-ATPase) AssayLite Antibody (PerCP Conjugate)
33239-05171	AssayPro	150 ug	EUR 565.2

Human ATPase Inhibitory Factor 1/IF1 AssayLite Antibody (RPE Conjugate)
33404-05151	AssayPro	150 ug	EUR 513.6

Human ATPase Inhibitory Factor 1/IF1 AssayLite Antibody (APC Conjugate)
33404-05161	AssayPro	150 ug	EUR 513.6

Human ATPase Inhibitory Factor 1/IF1 AssayLite Antibody (FITC Conjugate)
33404-05141	AssayPro	150 ug	EUR 513.6

Human ATPase Inhibitory Factor 1/IF1 AssayLite Antibody (PerCP Conjugate)
33404-05171	AssayPro	150 ug	EUR 565.2

ATPase, Na+/K+ Transporting (ATPase) Antibody
abx011699-100ug	Abbexa	100 ug	EUR 510

Rat Na+/K+ ATPase (Na/K ATPase) ELISA kit
E02N0538-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Na+/K+ ATPase (Na/K ATPase) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.