Summary
Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour arker it strongly recognises the most cancers particular T antigen (Galbeta1–>3GalNAc-), however not its sialylated model. Nonetheless, a further specificity in the direction of Galbeta1–>4GlcNAc (LacNAc), which isn’t tumour particular, had been attributed to PNA.
For proper interpretation of lectin histochemical outcomes we examined PNA sugar specificity utilizing naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, reasonably than mono- or disaccharides as ligands. Dot-blots, switch blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and different lectins of recognized specificity. Modifications of PNA-binding glycoproteins, together with selective removing of O-linked oligosaccharides and therapy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) in addition to uncovered T antigen (Galbeta1–>Three GalNAc-) was wonderful as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Outcomes have been confirmed utilizing TAG-specific human serum anti-alpha-galactoside antibody.
Arachis hypogaea (Peanut) Lectin (PNA) – HRP
Arachis hypogaea (PNA, Peanut Agglutinin) (HRP) |
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MBS655859-05mg | MyBiosource | 0.5mg | EUR 505 |
Arachis hypogaea (PNA, Peanut Agglutinin) (HRP) |
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MBS655859-1mg | MyBiosource | 1mg | EUR 895 |
Arachis hypogaea (PNA, Peanut Agglutinin) (HRP) |
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MBS655859-5x1mg | MyBiosource | 5x1mg | EUR 3885 |
Arachis hypogaea (PNA, Peanut Agglutinin) (HRP) |
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MBS614645-1mL | MyBiosource | 1mL | EUR 565 |
Arachis hypogaea (PNA, Peanut Agglutinin) (HRP) |
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MBS614645-5x1mL | MyBiosource | 5x1mL | EUR 2395 |
Arachis hypogaea Conglutin-7 |
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1-CSB-EP757666ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Conglutin-7 expressed in E.coli |
Arachis hypogaea lectin (PNA) |
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05-0116-10 | Medicago | 10 mg | EUR 139.2 |
Arachis hypogaea lectin (PNA |
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05-0116-1000 | Medicago | 1 g | Ask for price |
Arachis hypogaea lectin (PNA |
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05-0116-50 | Medicago | 50 mg | EUR 376.8 |
Arachis hypogaea 2S protein |
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MBS717441-10mg | MyBiosource | 10mg | EUR 1360 |
Arachis hypogaea 2S protein |
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MBS717441-1mg | MyBiosource | 1mg | EUR 230 |
Arachis hypogaea 2S protein |
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MBS717441-5x10mg | MyBiosource | 5x10mg | EUR 6075 |
rAra h 1 (Arachis hypogaea 1.0101) |
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10-P202-C100 | ExBio | 0.1mg | EUR 242 |
rAra h 2 (Arachis hypogaea 2.0201) |
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10-P208-C100 | ExBio | 0.1mg | EUR 242 |
rAra h 8 (Arachis hypogaea 8.0101) |
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10-P210-C100 | ExBio | 0.1mg | EUR 242 |
rAra h 9 (Arachis hypogaea 9.0101) |
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10-P232-C100 | ExBio | 0.1mg | EUR 242 |