The isolation and identification of most cancers stem cells are the important thing factors in exploring traits of most cancers stem cells at current. A number of species of most cancers stem cells, for example, retinoblastoma tumor stem cells, most cancers stem cells of melanoma of choroid, breast most cancers stem cells, lung most cancers stem cells, colon most cancers stem cells, and many others., have been remoted and cultured efficiently by serum free medium whereas their organic capabilities and traits are acquired.
This overview concentrate on the appliance of serum free medium within the analysis of isolating most cancers stem cells, each ocular and common, by way of offering basis for additional analysis on ocular most cancers stem cells.
A generic analysis platform with 2-dimensional (2D) cell tradition know-how, a third-dimensional (3D) in vitro tissue mannequin, and a scaled-down cell tradition and imaging system in between, was utilized to deal with the problematic points related to using serum in pores and skin tissue engineering. Human dermal fibroblasts (HDFs) and immortalized keratinocytes (HaCat cells) mono- or co-cultured in serum or serum-free medium had been in contrast and analyzed by way of the platform.

- It was demonstrated that serum depletion had important affect on the attachment of HaCat cells onto tissue tradition plastic (TCP), porous substrates and cellulosic scaffolds, which was additional enhanced by the pre-seeded HDFs. The advanced constructions fashioned by the HDFs colonized inside the porous substrates and scaffolds not solely prevented the seeded HaCat cells from filtering via the open pores, but additionally acted as mobile substrates for HaCat cells to connect onto.
- When mono-cultured on TCP, each HDFs and HaCat cells had been much less proliferative in medium with out serum than with serum. Nonetheless, each cell sorts had been efficiently co-cultured in 2D utilizing serum-free medium if the preliminary cell seeding density was greater than 80,000 cells/cm² (with 1:1 ratio). Primarily based on the outcomes from 2D cultures, co-culture of each cell sorts on modular substrates with small open pores (125 μm) and cellulosic scaffolds with open pores of various sizes (50-300 µm) had been then carried out efficiently in serum-free medium.
Mouse LIF ELISA Kit |
EML0014 |
Abclonal |
96Tests |
EUR 625.2 |
Mouse LIF shRNA Plasmid |
20-abx971321 |
Abbexa |
|
|
|
Recombinant Mouse LIF Protein |
R01400-1 |
BosterBio |
100ug/vial |
EUR 352.8 |
ELISA kit for Mouse LIF |
EK5250 |
SAB |
96 tests |
EUR 663.6 |
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse LIF in samples from serum, plasma, tissue homogenates and other biological fluids. |
Mouse LIF PicoKine ELISA Kit |
EK0580 |
BosterBio |
96 wells |
EUR 510 |
Description: For quantitative detection of mouse LIF in cell culture supernates, serum and plasma (heparin, EDTA). |
Lif ORF Vector (Mouse) (pORF) |
ORF049145 |
ABM |
1.0 ug DNA |
EUR 607.2 |
Lif ORF Vector (Mouse) (pORF) |
ORF049146 |
ABM |
1.0 ug DNA |
EUR 607.2 |
LIF ELISA Kit (Mouse) (OKBB00760) |
OKBB00760 |
Aviva Systems Biology |
96 Wells |
EUR 606 |
Description: Description of target: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml |
LIF ELISA Kit (Mouse) (OKEH04045) |
OKEH04045 |
Aviva Systems Biology |
96 Wells |
EUR 652.8 |
Description: Description of target: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.039 ng/mL |
LIF, Mouse Recombinant , 1MIU/ml |
L4500-001 |
GenDepot |
1ml |
EUR 406.8 |
LIF, Mouse Recombinant , 1MIU/ml |
L4500-005 |
GenDepot |
5x1ml |
EUR 1431.6 |
LIF, Mouse Recombinant , 10MIU/ml |
L4501-001 |
GenDepot |
1ml |
EUR 1744.8 |
Human CellExp? LIF, mouse recombinant |
7268-10 |
Biovision |
each |
EUR 444 |
Leukemia Inhibitory Factor (LIF), mouse |
PR16102 |
Neuromics |
5 ug |
EUR 522 |
Lif sgRNA CRISPR Lentivector set (Mouse) |
K3872501 |
ABM |
3 x 1.0 ug |
EUR 406.8 |
LIF Antibody |
25283-100ul |
SAB |
100ul |
EUR 468 |
LIF Antibody |
6245-002mg |
ProSci |
0.02 mg |
EUR 206.18 |
|
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation. |
LIF Antibody |
6245-01mg |
ProSci |
0.1 mg |
EUR 523.7 |
|
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation. |
LIF antibody |
70R-12299 |
Fitzgerald |
100 ug |
EUR 483.6 |
Description: Rabbit polyclonal LIF antibody |
LIF antibody |
70R-14100 |
Fitzgerald |
100 ug |
EUR 386.4 |
Description: Affinity purified Rabbit polyclonal LIF antibody |
LIF antibody |
70R-6230 |
Fitzgerald |
50 ug |
EUR 560.4 |
Description: Rabbit polyclonal LIF antibody |
LIF antibody |
70R-51295 |
Fitzgerald |
100 ul |
EUR 292.8 |
Description: Purified Polyclonal LIF antibody |
LIF Antibody |
36950-100ul |
SAB |
100ul |
EUR 302.4 |
LIF Antibody |
5216-100 |
Biovision |
each |
EUR 379.2 |
LIF Antibody |
5216-30T |
Biovision |
each |
EUR 175.2 |
LIF Antibody |
1-CSB-PA004761 |
Cusabio |
|
|
|
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000 |
LIF Antibody |
1-CSB-PA198481 |
Cusabio |
|
|
|
Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100 |
LIF Antibody |
1-CSB-PA012928ESR2HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: ELISA |
LIF Antibody |
F49998-0.08ML |
NSJ Bioreagents |
0.08 ml |
EUR 140.25 |
Description: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. |
LIF Antibody |
F49998-0.4ML |
NSJ Bioreagents |
0.4 ml |
EUR 322.15 |
Description: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. |
LIF Antibody |
R36251-100UG |
NSJ Bioreagents |
100 ug |
EUR 339.15 |
Description: Additional name(s) for this target protein: Leukemia inhibitory factor |
LIF Antibody |
R30639 |
NSJ Bioreagents |
100 ug |
EUR 356.15 |
Description: LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. It is produced in high amounts by the human endometrium and the trophoblast itself, and its receptors are present on cytotrophoblast cells. LIF could thus play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface. The gene maps to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. |
LIF Antibody |
R31467 |
NSJ Bioreagents |
100 ug |
EUR 356.15 |
Description: LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. This gene is mapped to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. It could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface. |
Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse) |
4-PAA085Mu01 |
Cloud-Clone |
-
EUR 283.20
-
EUR 2805.60
-
EUR 703.20
-
EUR 352.80
-
EUR 250.80
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF) |
anti-LIF |
YF-PA12952 |
Abfrontier |
50 ug |
EUR 435.6 |
Description: Mouse polyclonal to LIF |
LIF, Leukemia inhibitory factor, murine (mouse) |
RC234-18 |
Bio Basic |
5ug |
EUR 125.26 |
|
Mouse Leukemia Inhibitory Factor (LIF) Protein |
abx262959-1mg |
Abbexa |
1 mg |
EUR 3801.6 |
|
Mouse Leukemia Inhibitory Factor (LIF) Protein |
abx262959-3x10g |
Abbexa |
3x10 µg |
EUR 393.6 |
|
Mouse Leukemia Inhibitory Factor (LIF) Protein |
abx262959-3x2g |
Abbexa |
3x2 µg |
EUR 276 |
|
Mouse Leukemia Inhibitory Factor (LIF) Protein |
20-abx067761 |
Abbexa |
-
EUR 794.40
-
EUR 326.40
-
EUR 2414.40
-
EUR 944.40
-
EUR 577.20
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
LIF Protein Vector (Mouse) (pPM-C-HA) |
PV196580 |
ABM |
500 ng |
EUR 723.6 |
LIF Protein Vector (Mouse) (pPM-C-HA) |
PV196584 |
ABM |
500 ng |
EUR 723.6 |
Recombinant Mouse Leukemia Inhibitory Factor/lIF |
C690-10ug |
Novoprotein |
10ug |
EUR 175.2 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4. |
Recombinant Mouse Leukemia Inhibitory Factor/lIF |
C690-1mg |
Novoprotein |
1mg |
EUR 2739.6 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4. |
Recombinant Mouse Leukemia Inhibitory Factor/lIF |
C690-500ug |
Novoprotein |
500ug |
EUR 1935.6 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4. |
Recombinant Mouse Leukemia Inhibitory Factor/lIF |
C690-50ug |
Novoprotein |
50ug |
EUR 363.6 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4. |
LIF Protein Vector (Mouse) (pPB-C-His) |
PV196578 |
ABM |
500 ng |
EUR 723.6 |
LIF Protein Vector (Mouse) (pPB-N-His) |
PV196579 |
ABM |
500 ng |
EUR 723.6 |
LIF Protein Vector (Mouse) (pPM-C-His) |
PV196581 |
ABM |
500 ng |
EUR 723.6 |
LIF Protein Vector (Mouse) (pPB-C-His) |
PV196582 |
ABM |
500 ng |
EUR 723.6 |
LIF Protein Vector (Mouse) (pPB-N-His) |
PV196583 |
ABM |
500 ng |
EUR 723.6 |
LIF Protein Vector (Mouse) (pPM-C-His) |
PV196585 |
ABM |
500 ng |
EUR 723.6 |
Mouse Lif/ Leukemia inhibitory factor ELISA Kit |
E1656Mo |
Sunlong |
1 Kit |
EUR 655.2 |
Mouse Lif(Leukemia inhibitory factor) ELISA Kit |
EM0237 |
FN Test |
96T |
EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 46.9pg/ml |
Mouse LIF Protein (Ser 24-Phe 203) [His] |
VAng-1946Lsx-20g |
Creative Biolabs |
20 µg |
EUR 588 |
Description: Mouse LIF protein, His tag, expressed in human 293 cells. (Uniprot ID: P09056) |
Mouse LIF Protein (Ser 24-Phe 203) [His] |
VAng-1946Lsx-50g |
Creative Biolabs |
50 µg |
EUR 1050 |
Description: Mouse LIF protein, His tag, expressed in human 293 cells. (Uniprot ID: P09056) |
Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), PE |
4-PAA085Mu01-PE |
Cloud-Clone |
-
EUR 339.60
-
EUR 2942.40
-
EUR 843.60
-
EUR 423.60
-
EUR 226.80
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with PE. |
Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), APC |
4-PAA085Mu01-APC |
Cloud-Clone |
-
EUR 394.80
-
EUR 3649.20
-
EUR 1024.80
-
EUR 499.20
-
EUR 254.40
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with APC. |
Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), Cy3 |
4-PAA085Mu01-Cy3 |
Cloud-Clone |
-
EUR 476.40
-
EUR 4815.60
-
EUR 1316.40
-
EUR 615.60
-
EUR 289.20
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with Cy3. |
Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), HRP |
4-PAA085Mu01-HRP |
Cloud-Clone |
-
EUR 362.40
-
EUR 3182.40
-
EUR 907.20
-
EUR 452.40
-
EUR 240.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with HRP. |
Recombinant Mouse LIF Protein, Untagged, E.coli-1mg |
QP4235-1mg |
EnQuireBio |
1mg |
EUR 3189.6 |
Recombinant Mouse LIF Protein, Untagged, E.coli-5ug |
QP4235-5ug |
EnQuireBio |
5ug |
EUR 163.2 |
Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), FITC |
4-PAA085Mu01-FITC |
Cloud-Clone |
-
EUR 339.60
-
EUR 2942.40
-
EUR 843.60
-
EUR 423.60
-
EUR 226.80
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with FITC. |
Recombinant Mouse LIF Protein, Untagged, E.coli-20ug |
QP4235-20ug |
EnQuireBio |
20ug |
EUR 284.4 |
Recombinant Mouse LIF Protein, Untagged, E.coli-100ug |
QP4235-100ug |
EnQuireBio |
100ug |
EUR 729.6 |
Recombinant Mouse LIF Protein, Untagged, E.coli-500ug |
QP4235-500ug |
EnQuireBio |
500ug |
EUR 1992 |
- This examine demonstrated that the generic analysis platform had nice potential for in-depth understanding of HDFs and HaCat cells cultivated in serum-free medium, which may inform the processes for manufacturing pores and skin cells or tissues for scientific purposes.
Comparability of Human Dermal Fibroblasts and HaCat Cells Cultured in Medium with or with out Serum by way of a Generic Tissue Engineering Analysis Platform.
- A generic analysis platform with 2-dimensional (2D) cell tradition know-how, a third-dimensional (3D) in vitro tissue mannequin, and a scaled-down cell tradition and imaging system in between, was utilized to deal with the problematic points related with using serum in pores and skin tissue engineering. Human dermal fibroblasts (HDFs) and immortalized keratinocytes (HaCat cells) mono- or co-cultured in serum or serum-free medium had been in contrast and analyzed by way of the platform.
Recombinant Mouse GRO/KC (CXCL1) |
7-01859 |
CHI Scientific |
20µg |
Ask for price |
Recombinant Mouse GRO/KC (CXCL1) |
7-01860 |
CHI Scientific |
1mg |
Ask for price |
GRO1/KC Mouse Recombinant Protein (CXCL1) |
PROTP12850-1 |
BosterBio |
Regular: 20ug |
EUR 380.4 |
Description: KC Mouse Recombinant also known as N51 and GRO1 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of approximately 8 kDa.;The GRO-1 is purified by proprietary chromatographic techniques. |
GRO1/KC Mouse, GRO/KC (CXCL1) Mouse Recombinant Protein, His Tag |
PROTP12850 |
BosterBio |
Regular: 20ug |
EUR 380.4 |
Description: GRO1/KC Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 97 amino acids (25-96 a.a.) and having a molecular mass of 10.5kDa.;GRO1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
GRO-alpha, KC, CXCL1 (rMuKC), murine (mouse) |
RC332-12 |
Bio Basic |
5ug |
EUR 125.26 |
|
GRO / KC (CXCL1) Protein |
20-abx260684 |
Abbexa |
-
EUR 4101.60
-
EUR 393.60
-
EUR 276.00
|
|
|
GRO / KC (CXCL1) Protein |
20-abx261870 |
Abbexa |
-
EUR 5388.00
-
EUR 393.60
-
EUR 276.00
|
|
|
GRO, KC, CXCL1, rRtGRO, rat |
RC352-12 |
Bio Basic |
5ug |
EUR 125.26 |
|
GRO/KC (CXCL1), Rat Recombinant |
P1288-25 |
Biovision |
each |
EUR 444 |
GRO/KC (CXCL1), Rat Recombinant |
P1288-5 |
Biovision |
each |
EUR 210 |
Recombinant Murine KC (CXCL1) Protein |
PROTP12850-2 |
BosterBio |
20ug |
EUR 380.4 |
Description: All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant murine KC is a 7.8 kDa protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2. |
Recombinant Rat GRO/KC (CXCL1) Protein |
PROTP14095-1 |
BosterBio |
25ug |
EUR 380.4 |
Description: All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant rat GRO/KC is a 7.8 kDa protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2. |
Rat CXCL1/CINC-1/KC ELISA Kit |
RK00196 |
Abclonal |
96 Tests |
EUR 625.2 |
Mouse CXCL1 ELISA Kit |
55R-1741 |
Fitzgerald |
1 kit |
EUR 748.8 |
Description: ELISA kit for detection of CXCL1 in the research laboratory |
Mouse CXCL1 shRNA Plasmid |
20-abx970673 |
Abbexa |
|
|
|
ELISA kit for Mouse CXCL1 |
EK5299 |
SAB |
96 tests |
EUR 663.6 |
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse CXCL1 in samples from serum, plasma, tissue homogenates and other biological fluids. |
Mouse CXCL1/GROα ELISA kit |
LF-EK50473 |
Abfrontier |
1×96T |
EUR 777.6 |
Mouse CXCL1 PicoKine ELISA Kit |
EK0723 |
BosterBio |
96 wells |
EUR 547.2 |
Description: For quantitative detection of mouse CXCL1 in cell culture supernates and serum. |
Mouse CXCL1 Detection Assay Kit |
6725 |
Chondrex |
1 kit |
EUR 580.26 |
Description: Mouse CXCL1 Detection Assay Kit |
CXCL1 protein (Mouse) (His tag) |
80R-3368 |
Fitzgerald |
50 ug |
EUR 308.4 |
Description: Purified recombinant CXCL1 protein (Mouse) (His tag) |
CXCL1 protein (Mouse) (His tag) |
80R-3439 |
Fitzgerald |
50 ug |
EUR 308.4 |
Description: Purified recombinant CXCL1 protein (Mouse) (His tag) |
Cxcl1 ORF Vector (Mouse) (pORF) |
ORF042286 |
ABM |
1.0 ug DNA |
EUR 114 |
Mouse CXCL1 AssayLite Antibody (FITC Conjugate) |
70027-05141 |
AssayPro |
150 ug |
EUR 513.6 |
CXCL1 ELISA Kit (Mouse) (OKCD05712) |
OKCD05712 |
Aviva Systems Biology |
96 Wells |
EUR 730.8 |
Description: Description of target: This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 6.0pg/mL |
CXCL1 ELISA Kit (Mouse) (OKBB00312) |
OKBB00312 |
Aviva Systems Biology |
96 Tests |
EUR 652.8 |
Description: Description of target: Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function. The standard product used in this kit is recombinant mouse CXCL1, consisting of 77 amino acids with the molecular mass of 8KDa.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 1 pg/ml |
CXCL1 ELISA Kit (Mouse) (OKAN04583) |
OKAN04583 |
Aviva Systems Biology |
96 Wells |
EUR 950.4 |
Description: Description of target: This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung.;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 6.0 pg/mL |
Mouse CXCL1/GROα ELISA kit (4X96T) |
LF-EK50474 |
Abfrontier |
4×96T |
EUR 2641.2 |
Anti-GRO alpha/Cxcl1 Antibody |
A00533 |
BosterBio |
100ug/vial |
EUR 352.8 |
Anti-GRO alpha/CXCL1 Antibody |
PA1760 |
BosterBio |
100ug/vial |
EUR 352.8 |
CXCL1 antibody |
70R-10502 |
Fitzgerald |
50 ug |
EUR 560.4 |
Description: Affinity purified rabbit polyclonal CXCL1 antibody |
CXCL1 Antibody |
43679-100ul |
SAB |
100ul |
EUR 302.4 |
CXCL1 antibody |
70R-16681 |
Fitzgerald |
50 ul |
EUR 522 |
Description: Rabbit polyclonal CXCL1 antibody |
CXCL1 antibody |
70R-14297 |
Fitzgerald |
100 ug |
EUR 392.4 |
Description: Affinity purified Rabbit polyclonal CXCL1 antibody |
CXCL1 antibody |
70R-15473 |
Fitzgerald |
100 ug |
EUR 392.4 |
Description: Rabbit polyclonal CXCL1 antibody |
CXCL1 Antibody |
33054-100ul |
SAB |
100ul |
EUR 302.4 |
CXCL1 Antibody |
1-CSB-PA009036 |
Cusabio |
|
|
|
Description: A polyclonal antibody against CXCL1. Recognizes CXCL1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/10000 |
Cxcl1 Antibody |
1-CSB-PA09217A0Rb |
Cusabio |
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Description: A polyclonal antibody against Cxcl1. Recognizes Cxcl1 from Mouse. This antibody is Unconjugated. Tested in the following application: ELISA |
CXCL1 Antibody |
1-CSB-PA006239GA01HU |
Cusabio |
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Description: A polyclonal antibody against CXCL1. Recognizes CXCL1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC |
CXCL1 Antibody |
1-CSB-PA06097A0Rb |
Cusabio |
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Description: A polyclonal antibody against CXCL1. Recognizes CXCL1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA |
GRO-alpha/CXCL1 (CHO-expressed), Mouse |
HY-P7186 |
MedChemExpress |
50ug |
EUR 404.4 |
Cxcl1 sgRNA CRISPR Lentivector set (Mouse) |
K4368201 |
ABM |
3 x 1.0 ug |
EUR 406.8 |
Mouse Growth-regulated alpha protein (Cxcl1) |
1-CSB-RP092174m |
Cusabio |
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EUR 606.00
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EUR 318.00
-
EUR 2192.40
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EUR 919.20
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EUR 1461.60
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EUR 402.00
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- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
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Description: Recombinant Mouse Growth-regulated alpha protein(Cxcl1),partial expressed in E.coli |
CXCL1 Protein Vector (Mouse) (pPM-C-HA) |
PV169144 |
ABM |
500 ng |
EUR 723.6 |
Cxcl1 ELISA Kit (Mouse) : 96 Wells (OKEH02714) |
OKEH02714 |
Aviva Systems Biology |
96 Wells |
EUR 652.8 |
Description: Description of target: This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung. ;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 15.76 pg/mL |
CXCL1 Protein Vector (Mouse) (pPB-C-His) |
PV169142 |
ABM |
500 ng |
EUR 723.6 |
CXCL1 Protein Vector (Mouse) (pPB-N-His) |
PV169143 |
ABM |
500 ng |
EUR 723.6 |
CXCL1 Protein Vector (Mouse) (pPM-C-His) |
PV169145 |
ABM |
500 ng |
EUR 723.6 |
CXCL1 antibody (HRP) |
60R-2236 |
Fitzgerald |
100 ug |
EUR 392.4 |
Description: Rabbit polyclonal CXCL1 antibody (HRP) |
CXCL1 antibody (FITC) |
60R-2237 |
Fitzgerald |
100 ug |
EUR 392.4 |
Description: Rabbit polyclonal CXCL1 antibody (FITC) |
CXCL1/GRO |
E21-597 |
EnoGene |
10ug |
EUR 411.6 |
Cxcl1 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
K4368202 |
ABM |
1.0 ug DNA |
EUR 184.8 |
Cxcl1 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
K4368203 |
ABM |
1.0 ug DNA |
EUR 184.8 |
Cxcl1 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
K4368204 |
ABM |
1.0 ug DNA |
EUR 184.8 |
CXCL1 antibody (biotin) |
60R-2238 |
Fitzgerald |
100 ug |
EUR 392.4 |
Description: Rabbit polyclonal CXCL1 antibody (biotin) |
CXCL1 siRNA |
20-abx913248 |
Abbexa |
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CXCL1 siRNA |
20-abx913249 |
Abbexa |
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CXCL1 siRNA |
20-abx901350 |
Abbexa |
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CXCL1 protein |
30R-3156 |
Fitzgerald |
50 ug |
EUR 308.4 |
Description: Purified recombinant CXCL1 protein |
CXCL1 Peptide |
42-129P |
ProSci |
0.1 mg |
EUR 405.6 |
Description: (CT) CXCL1 Peptide |
Cxcl1 Polyclonal Antibody |
A51950 |
EpiGentek |
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EUR 684.66
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EUR 117.70
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EUR 302.50
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EUR 423.50
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- 100 µg
- 20 ul
- 50 ul
- 100 ul
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CXCL1 Polyclonal Antibody |
A51978 |
EpiGentek |
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EUR 684.66
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EUR 117.70
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EUR 302.50
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EUR 423.50
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- 100 µg
- 20 ul
- 50 ul
- 100 ul
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CXCL1 Conjugated Antibody |
C33054 |
SAB |
100ul |
EUR 476.4 |
CXCL1 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03823) |
OKCD03823 |
Aviva Systems Biology |
96 Wells |
EUR 1232.4 |
Description: Description of target: Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation . Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. KC(5-72) shows a highly enhanced hematopoietic activity.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 15.6 pg/mL |
Mouse Cxcl1/ Growth-regulated alpha protein ELISA Kit |
E0354Mo |
Sunlong |
1 Kit |
EUR 655.2 |
Mouse Growth regulated alpha protein, Cxcl1 ELISA KIT |
ELI-04012m |
Lifescience Market |
96 Tests |
EUR 1038 |
GRO alpha (CXCL1) Antibody |
abx018005-100ug |
Abbexa |
100 ug |
EUR 410.4 |
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Gro alpha Antibody / CXCL1 |
RQ4031 |
NSJ Bioreagents |
100 ug |
EUR 356.15 |
Description: CXCL1 (Chemokine, CXC motif, Ligand 1), also called GRO1, SCYB1, GROA or MGSA, is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GRO?, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-?). In humans, this protein is encoded by the CXCL1 gene. The CXCL1 gene is mapped on 4q13.3. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. It is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis. Signaling through Cxcr2, Cxcl1 inhibited oligodendrocyte precursor migration. The migrational arrest was rapid, reversible, and concentration dependent, and it reflected enhanced cell/substrate interactions. White matter expression of Cxcl1 was temporospatially regulated. Others contribute to aggressive growth selectivity in the lung. Among the lung metastasis signature genes identified, several, including CXCL1, were functionally validated. |
Mouse Growth-Regulated Alpha Protein (CXCL1) ELISA Kit |
20-abx154438 |
Abbexa |
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EUR 6373.20
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EUR 3404.40
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EUR 801.60
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- 10 × 96 tests
- 5 × 96 tests
- 96 tests
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Mouse Growth-Regulated Alpha Protein (CXCL1) ELISA Kit |
abx050043-96tests |
Abbexa |
96 tests |
EUR 924 |
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Mouse Growth-Regulated Alpha Protein (CXCL1) ELISA Kit |
abx253956-96tests |
Abbexa |
96 tests |
EUR 801.6 |
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Mouse Growth-Regulated Alpha Protein (CXCL1) ELISA Kit |
abx574645-96tests |
Abbexa |
96 tests |
EUR 801.6 |
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Mouse CXCL1/GROα(Growth Regulated Oncogene Alpha) ELISA Kit |
EM0003 |
FN Test |
96T |
EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 9.375pg/ml |
Cxcl1 Antibody, HRP conjugated |
1-CSB-PA09217B0Rb |
Cusabio |
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Description: A polyclonal antibody against Cxcl1. Recognizes Cxcl1 from Mouse. This antibody is HRP conjugated. Tested in the following application: ELISA |
CXCL1 Antibody, HRP conjugated |
1-CSB-PA06097B0Rb |
Cusabio |
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Description: A polyclonal antibody against CXCL1. Recognizes CXCL1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
Cxcl1 Antibody, FITC conjugated |
1-CSB-PA09217C0Rb |
Cusabio |
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Description: A polyclonal antibody against Cxcl1. Recognizes Cxcl1 from Mouse. This antibody is FITC conjugated. Tested in the following application: ELISA |
CXCL1 Antibody, FITC conjugated |
1-CSB-PA06097C0Rb |
Cusabio |
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Description: A polyclonal antibody against CXCL1. Recognizes CXCL1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
- It was demonstrated that serum depletion had important affect on the attachment of HaCat cells onto tissue tradition plastic (TCP), porous substrates and cellulosic scaffolds, which was additional enhanced by the pre-seeded HDFs. The advanced constructions fashioned by the HDFs colonized inside the porous substrates and scaffolds not solely prevented the seeded HaCat cells from filtering via the open pores, but additionally acted as mobile substrates for HaCat cells to connect onto.
- When mono-cultured on TCP, each HDFs and HaCat cells had been much less proliferative in medium with out serum than with serum. Nonetheless, each cell sorts had been efficiently co-cultured in 2D utilizing serum-free medium if the preliminary cell seeding density was greater than 80,000 cells/cm² (with 1:1 ratio).
- Primarily based on the outcomes from 2D cultures, co-culture of each cell sorts on modular substrates with small open pores (125 μm) and cellulosic scaffolds with open pores of various sizes (50-300 µm) had been then carried out efficiently in serum-free medium. This examine demonstrated that the generic analysis platform had nice potential for in-depth understanding of HDFs and HaCat cells cultivated in serum-free medium, which may inform the processes for manufacturing pores and skin cells or tissues for scientific purposes.