gentur

The application of serum free medium in the research of isolating cancer stem cells

The isolation and identification of most cancers stem cells are the important thing factors in exploring traits of most cancers stem cells at current. A number of species of most cancers stem cells, for example, retinoblastoma tumor stem cells, most cancers stem cells of melanoma of choroid, breast most cancers stem cells, lung most cancers stem cells, colon most cancers stem cells, and many others., have been remoted and cultured efficiently by serum free medium whereas their organic capabilities and traits are acquired.

 

This overview concentrate on the appliance of serum free medium within the analysis of isolating most cancers stem cells, each ocular and common, by way of offering basis for additional analysis on ocular most cancers stem cells.

 

A generic analysis platform with 2-dimensional (2D) cell tradition know-how, a third-dimensional (3D) in vitro tissue mannequin, and a scaled-down cell tradition and imaging system in between, was utilized to deal with the problematic points related to using serum in pores and skin tissue engineering. Human dermal fibroblasts (HDFs) and immortalized keratinocytes (HaCat cells) mono- or co-cultured in serum or serum-free medium had been in contrast and analyzed by way of the platform.

 

gentur

  • It was demonstrated that serum depletion had important affect on the attachment of HaCat cells onto tissue tradition plastic (TCP), porous substrates and cellulosic scaffolds, which was additional enhanced by the pre-seeded HDFs. The advanced constructions fashioned by the HDFs colonized inside the porous substrates and scaffolds not solely prevented the seeded HaCat cells from filtering via the open pores, but additionally acted as mobile substrates for HaCat cells to connect onto.

 

  • When mono-cultured on TCP, each HDFs and HaCat cells had been much less proliferative in medium with out serum than with serum. Nonetheless, each cell sorts had been efficiently co-cultured in 2D utilizing serum-free medium if the preliminary cell seeding density was greater than 80,000 cells/cm² (with 1:1 ratio). Primarily based on the outcomes from 2D cultures, co-culture of each cell sorts on modular substrates with small open pores (125 μm) and cellulosic scaffolds with open pores of various sizes (50-300 µm) had been then carried out efficiently in serum-free medium.

Mouse LIF ELISA Kit

EML0014 96Tests
EUR 625.2

Mouse LIF shRNA Plasmid

20-abx971321
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Recombinant Mouse LIF Protein

R01400-1 100ug/vial
EUR 352.8

ELISA kit for Mouse LIF

EK5250 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse LIF in samples from serum, plasma, tissue homogenates and other biological fluids.

Mouse LIF PicoKine ELISA Kit

EK0580 96 wells
EUR 510
Description: For quantitative detection of mouse LIF in cell culture supernates, serum and plasma (heparin, EDTA).

Lif ORF Vector (Mouse) (pORF)

ORF049145 1.0 ug DNA
EUR 607.2

Lif ORF Vector (Mouse) (pORF)

ORF049146 1.0 ug DNA
EUR 607.2

LIF ELISA Kit (Mouse) (OKBB00760)

OKBB00760 96 Wells
EUR 606
Description: Description of target: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml

LIF ELISA Kit (Mouse) (OKEH04045)

OKEH04045 96 Wells
EUR 652.8
Description: Description of target: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.039 ng/mL

LIF, Mouse Recombinant , 1MIU/ml

L4500-001 1ml
EUR 406.8

LIF, Mouse Recombinant , 1MIU/ml

L4500-005 5x1ml
EUR 1431.6

LIF, Mouse Recombinant , 10MIU/ml

L4501-001 1ml
EUR 1744.8

Nori® Mouse LIF ELISA Kit

GR117058 96-well
EUR 461

Human CellExp? LIF, mouse recombinant

7268-10 each
EUR 444

Leukemia Inhibitory Factor (LIF), mouse

PR16102 5 ug
EUR 522

Lif sgRNA CRISPR Lentivector set (Mouse)

K3872501 3 x 1.0 ug
EUR 406.8

LIF Antibody

25283-100ul 100ul
EUR 468

LIF Antibody

6245-002mg 0.02 mg
EUR 206.18
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

LIF Antibody

6245-01mg 0.1 mg
EUR 523.7
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

LIF antibody

70R-12299 100 ug
EUR 483.6
Description: Rabbit polyclonal LIF antibody

LIF antibody

70R-14100 100 ug
EUR 386.4
Description: Affinity purified Rabbit polyclonal LIF antibody

LIF antibody

70R-6230 50 ug
EUR 560.4
Description: Rabbit polyclonal LIF antibody

LIF antibody

70R-51295 100 ul
EUR 292.8
Description: Purified Polyclonal LIF antibody

LIF Antibody

36950-100ul 100ul
EUR 302.4

LIF Antibody

5216-100 each
EUR 379.2

LIF Antibody

5216-30T each
EUR 175.2

LIF Antibody

1-CSB-PA004761
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

LIF Antibody

1-CSB-PA198481
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100

LIF Antibody

DF13730 100ul
EUR 420

LIF Antibody

1-CSB-PA012928ESR2HU
  • EUR 266.40
  • EUR 402.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: ELISA

LIF Antibody

F49998-0.08ML 0.08 ml
EUR 140.25
Description: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.

LIF Antibody

F49998-0.4ML 0.4 ml
EUR 322.15
Description: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.

LIF antibody

PAab09804 100 ug
EUR 463.2

LIF Antibody

R36251-100UG 100 ug
EUR 339.15
Description: Additional name(s) for this target protein: Leukemia inhibitory factor

LIF Antibody

R30639 100 ug
EUR 356.15
Description: LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. It is produced in high amounts by the human endometrium and the trophoblast itself, and its receptors are present on cytotrophoblast cells. LIF could thus play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface. The gene maps to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2.

LIF Antibody

R31467 100 ug
EUR 356.15
Description: LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. This gene is mapped to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. It could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface.

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse)

4-PAA085Mu01
  • EUR 283.20
  • EUR 2805.60
  • EUR 703.20
  • EUR 352.80
  • EUR 250.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF)

anti-LIF

YF-PA12952 50 ug
EUR 435.6
Description: Mouse polyclonal to LIF

LIF, Leukemia inhibitory factor, murine (mouse)

RC234-18 5ug
EUR 125.26

Mouse Leukemia Inhibitory Factor (LIF) Protein

abx262959-1mg 1 mg
EUR 3801.6

Mouse Leukemia Inhibitory Factor (LIF) Protein

abx262959-3x10g 3x10 µg
EUR 393.6

Mouse Leukemia Inhibitory Factor (LIF) Protein

abx262959-3x2g 3x2 µg
EUR 276

Mouse Leukemia Inhibitory Factor (LIF) Protein

20-abx067761
  • EUR 794.40
  • EUR 326.40
  • EUR 2414.40
  • EUR 944.40
  • EUR 577.20
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

LIF Protein Vector (Mouse) (pPM-C-HA)

PV196580 500 ng
EUR 723.6

LIF Protein Vector (Mouse) (pPM-C-HA)

PV196584 500 ng
EUR 723.6

Recombinant Mouse Leukemia Inhibitory Factor/lIF

C690-10ug 10ug
EUR 175.2
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Mouse Leukemia Inhibitory Factor/lIF

C690-1mg 1mg
EUR 2739.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Mouse Leukemia Inhibitory Factor/lIF

C690-500ug 500ug
EUR 1935.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Mouse Leukemia Inhibitory Factor/lIF

C690-50ug 50ug
EUR 363.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

LIF Protein Vector (Mouse) (pPB-C-His)

PV196578 500 ng
EUR 723.6

LIF Protein Vector (Mouse) (pPB-N-His)

PV196579 500 ng
EUR 723.6

LIF Protein Vector (Mouse) (pPM-C-His)

PV196581 500 ng
EUR 723.6

LIF Protein Vector (Mouse) (pPB-C-His)

PV196582 500 ng
EUR 723.6

LIF Protein Vector (Mouse) (pPB-N-His)

PV196583 500 ng
EUR 723.6

LIF Protein Vector (Mouse) (pPM-C-His)

PV196585 500 ng
EUR 723.6

Mouse Lif/ Leukemia inhibitory factor ELISA Kit

E1656Mo 1 Kit
EUR 655.2

Mouse Lif(Leukemia inhibitory factor) ELISA Kit

EM0237 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 46.9pg/ml

Mouse LIF Protein (Ser 24-Phe 203) [His]

VAng-1946Lsx-20g 20 µg
EUR 588
Description: Mouse LIF protein, His tag, expressed in human 293 cells. (Uniprot ID: P09056)

Mouse LIF Protein (Ser 24-Phe 203) [His]

VAng-1946Lsx-50g 50 µg
EUR 1050
Description: Mouse LIF protein, His tag, expressed in human 293 cells. (Uniprot ID: P09056)

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), PE

4-PAA085Mu01-PE
  • EUR 339.60
  • EUR 2942.40
  • EUR 843.60
  • EUR 423.60
  • EUR 226.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with PE.

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), APC

4-PAA085Mu01-APC
  • EUR 394.80
  • EUR 3649.20
  • EUR 1024.80
  • EUR 499.20
  • EUR 254.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with APC.

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), Cy3

4-PAA085Mu01-Cy3
  • EUR 476.40
  • EUR 4815.60
  • EUR 1316.40
  • EUR 615.60
  • EUR 289.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with Cy3.

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), HRP

4-PAA085Mu01-HRP
  • EUR 362.40
  • EUR 3182.40
  • EUR 907.20
  • EUR 452.40
  • EUR 240.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with HRP.

Recombinant Mouse LIF Protein, Untagged, E.coli-1mg

QP4235-1mg 1mg
EUR 3189.6

Recombinant Mouse LIF Protein, Untagged, E.coli-5ug

QP4235-5ug 5ug
EUR 163.2

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), FITC

4-PAA085Mu01-FITC
  • EUR 339.60
  • EUR 2942.40
  • EUR 843.60
  • EUR 423.60
  • EUR 226.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with FITC.

Recombinant Mouse LIF Protein, Untagged, E.coli-20ug

QP4235-20ug 20ug
EUR 284.4

Recombinant Mouse LIF Protein, Untagged, E.coli-100ug

QP4235-100ug 100ug
EUR 729.6

Recombinant Mouse LIF Protein, Untagged, E.coli-500ug

QP4235-500ug 500ug
EUR 1992
  • This examine demonstrated that the generic analysis platform had nice potential for in-depth understanding of HDFs and HaCat cells cultivated in serum-free medium, which may inform the processes for manufacturing pores and skin cells or tissues for scientific purposes.

Comparability of Human Dermal Fibroblasts and HaCat Cells Cultured in Medium with or with out Serum by way of a Generic Tissue Engineering Analysis Platform.

  • A generic analysis platform with 2-dimensional (2D) cell tradition know-how, a third-dimensional (3D) in vitro tissue mannequin, and a scaled-down cell tradition and imaging system in between, was utilized to deal with the problematic points related with using serum in pores and skin tissue engineering. Human dermal fibroblasts (HDFs) and immortalized keratinocytes (HaCat cells) mono- or co-cultured in serum or serum-free medium had been in contrast and analyzed by way of the platform.

Recombinant Mouse GRO/KC (CXCL1)

7-01859 20µg Ask for price

Recombinant Mouse GRO/KC (CXCL1)

7-01860 1mg Ask for price

Recombinant Mouse (E.Coli) GRO/KC (CXCL1)

RP-1037 5 ug
EUR 196.8

GRO1/KC Mouse Recombinant Protein (CXCL1)

PROTP12850-1 Regular: 20ug
EUR 380.4
Description: KC Mouse Recombinant also known as N51 and GRO1 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of approximately 8 kDa.;The GRO-1 is purified by proprietary chromatographic techniques.

GRO1/KC Mouse, GRO/KC (CXCL1) Mouse Recombinant Protein, His Tag

PROTP12850 Regular: 20ug
EUR 380.4
Description: GRO1/KC Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 97 amino acids (25-96 a.a.) and having a molecular mass of 10.5kDa.;GRO1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

GRO-alpha, KC, CXCL1 (rMuKC), murine (mouse)

RC332-12 5ug
EUR 125.26

GRO / KC (CXCL1) Protein

20-abx260684
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

GRO / KC (CXCL1) Protein

20-abx261870
  • EUR 5388.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

GRO, KC, CXCL1, rRtGRO, rat

RC352-12 5ug
EUR 125.26

Mouse CXC-chemokine ligand 1(CXCL1/KC)ELISA Kit

YLA0122MO-48T 48T
EUR 465

Mouse CXC-chemokine ligand 1(CXCL1/KC)ELISA Kit

YLA0122MO-96T 96T
EUR 600

GRO/KC (CXCL1), Rat Recombinant

P1288-25 each
EUR 444

GRO/KC (CXCL1), Rat Recombinant

P1288-5 each
EUR 210

Recombinant Murine KC (CXCL1) Protein

PROTP12850-2 20ug
EUR 380.4
Description: All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant murine KC is a 7.8 kDa protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2.

Recombinant Rat GRO/KC (CXCL1) Protein

PROTP14095-1 25ug
EUR 380.4
Description: All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant rat GRO/KC is a 7.8 kDa protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2.

Rat CXCL1/CINC-1/KC ELISA Kit

RK00196 96 Tests
EUR 625.2

Rabbit Anti Mouse Cxcl1 Polyclonal Antibody

CPBT-65100RM 0.1 mg
EUR 1057.2

Mouse CXCL1 ELISA Kit

55R-1741 1 kit
EUR 748.8
Description: ELISA kit for detection of CXCL1 in the research laboratory

Mouse CXCL1 shRNA Plasmid

20-abx970673
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

GRO-alpha/CXCL1, Mouse

HY-P7188 50ug
EUR 639.6

ELISA kit for Mouse CXCL1

EK5299 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse CXCL1 in samples from serum, plasma, tissue homogenates and other biological fluids.

Mouse CXCL1/GROα ELISA kit

LF-EK50473 1×96T
EUR 777.6

Mouse CXCL1 PicoKine ELISA Kit

EK0723 96 wells
EUR 547.2
Description: For quantitative detection of mouse CXCL1 in cell culture supernates and serum.

Mouse CXCL1 Detection Assay Kit

6725 1 kit
EUR 580.26
Description: Mouse CXCL1 Detection Assay Kit

CXCL1 protein (Mouse) (His tag)

80R-3368 50 ug
EUR 308.4
Description: Purified recombinant CXCL1 protein (Mouse) (His tag)

CXCL1 protein (Mouse) (His tag)

80R-3439 50 ug
EUR 308.4
Description: Purified recombinant CXCL1 protein (Mouse) (His tag)

Cxcl1 ORF Vector (Mouse) (pORF)

ORF042286 1.0 ug DNA
EUR 114

Mouse CXCL1 AssayLite Antibody (FITC Conjugate)

70027-05141 150 ug
EUR 513.6

CXCL1 ELISA Kit (Mouse) (OKCD05712)

OKCD05712 96 Wells
EUR 730.8
Description: Description of target: This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 6.0pg/mL

CXCL1 ELISA Kit (Mouse) (OKBB00312)

OKBB00312 96 Tests
EUR 652.8
Description: Description of target: Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function. The standard product used in this kit is recombinant mouse CXCL1, consisting of 77 amino acids with the molecular mass of 8KDa.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 1 pg/ml

CXCL1 ELISA Kit (Mouse) (OKAN04583)

OKAN04583 96 Wells
EUR 950.4
Description: Description of target: This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung.;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 6.0 pg/mL

Mouse CXCL1/GROα ELISA kit (4X96T)

LF-EK50474 4×96T
EUR 2641.2

Anti-GRO alpha/Cxcl1 Antibody

A00533 100ug/vial
EUR 352.8

Anti-GRO alpha/CXCL1 Antibody

PA1760 100ug/vial
EUR 352.8

CXCL1 antibody

70R-10502 50 ug
EUR 560.4
Description: Affinity purified rabbit polyclonal CXCL1 antibody

CXCL1 Antibody

43679-100ul 100ul
EUR 302.4

CXCL1 antibody

70R-16681 50 ul
EUR 522
Description: Rabbit polyclonal CXCL1 antibody

CXCL1 antibody

70R-14297 100 ug
EUR 392.4
Description: Affinity purified Rabbit polyclonal CXCL1 antibody

CXCL1 antibody

70R-15473 100 ug
EUR 392.4
Description: Rabbit polyclonal CXCL1 antibody

CXCL1 Antibody

33054-100ul 100ul
EUR 302.4

CXCL1 Antibody

1-CSB-PA009036
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against CXCL1. Recognizes CXCL1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/10000

Cxcl1 Antibody

1-CSB-PA09217A0Rb
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Cxcl1. Recognizes Cxcl1 from Mouse. This antibody is Unconjugated. Tested in the following application: ELISA

CXCL1 Antibody

1-CSB-PA006239GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against CXCL1. Recognizes CXCL1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

CXCL1 Antibody

1-CSB-PA06097A0Rb
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against CXCL1. Recognizes CXCL1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA

GRO-alpha/CXCL1 (CHO-expressed), Mouse

HY-P7186 50ug
EUR 404.4

Cxcl1 sgRNA CRISPR Lentivector set (Mouse)

K4368201 3 x 1.0 ug
EUR 406.8

Mouse Growth-regulated alpha protein (Cxcl1)

1-CSB-RP092174m
  • EUR 606.00
  • EUR 318.00
  • EUR 2192.40
  • EUR 919.20
  • EUR 1461.60
  • EUR 402.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Growth-regulated alpha protein(Cxcl1),partial expressed in E.coli

CXCL1 Protein Vector (Mouse) (pPM-C-HA)

PV169144 500 ng
EUR 723.6

Cxcl1 ELISA Kit (Mouse) : 96 Wells (OKEH02714)

OKEH02714 96 Wells
EUR 652.8
Description: Description of target: This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung. ;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 15.76 pg/mL

CXCL1 Protein Vector (Mouse) (pPB-C-His)

PV169142 500 ng
EUR 723.6

CXCL1 Protein Vector (Mouse) (pPB-N-His)

PV169143 500 ng
EUR 723.6

CXCL1 Protein Vector (Mouse) (pPM-C-His)

PV169145 500 ng
EUR 723.6

Rabbit Anti Rat Cxcl1 Polyclonal Antibody

CPBT-65101RR 0.1 mg
EUR 1057.2

Nori® Mouse CXCL1/GRO-alpha ELISA Kit

GR117324 96-well
EUR 461

CXCL1 antibody (HRP)

60R-2236 100 ug
EUR 392.4
Description: Rabbit polyclonal CXCL1 antibody (HRP)

CXCL1 antibody (FITC)

60R-2237 100 ug
EUR 392.4
Description: Rabbit polyclonal CXCL1 antibody (FITC)

CXCL1/GRO

E21-597 10ug
EUR 411.6

CXCL1/GRO

EF012822 96 Tests
EUR 826.8

Cxcl1 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4368202 1.0 ug DNA
EUR 184.8

Cxcl1 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4368203 1.0 ug DNA
EUR 184.8

Cxcl1 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4368204 1.0 ug DNA
EUR 184.8

CXCL1 antibody (biotin)

60R-2238 100 ug
EUR 392.4
Description: Rabbit polyclonal CXCL1 antibody (biotin)

CXCL1 siRNA

20-abx913248
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

CXCL1 siRNA

20-abx913249
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

CXCL1 siRNA

20-abx901350
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

pEGFP- CXCL1

PVT10178 2 ug
EUR 361.2

CXCL1 protein

30R-3156 50 ug
EUR 308.4
Description: Purified recombinant CXCL1 protein

CXCL1 Peptide

42-129P 0.1 mg
EUR 405.6
Description: (CT) CXCL1 Peptide

Cxcl1 Polyclonal Antibody

A51950
  • EUR 684.66
  • EUR 117.70
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

CXCL1 Polyclonal Antibody

A51978
  • EUR 684.66
  • EUR 117.70
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

CXCL1 Conjugated Antibody

C33054 100ul
EUR 476.4

CXCL1 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03823)

OKCD03823 96 Wells
EUR 1232.4
Description: Description of target: Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation . Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. KC(5-72) shows a highly enhanced hematopoietic activity.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 15.6 pg/mL

Mouse Cxcl1/ Growth-regulated alpha protein ELISA Kit

E0354Mo 1 Kit
EUR 655.2

Mouse Growth regulated alpha protein, Cxcl1 ELISA KIT

ELI-04012m 96 Tests
EUR 1038

GRO alpha (CXCL1) Antibody

abx018005-100ug 100 ug
EUR 410.4

Gro alpha Antibody / CXCL1

RQ4031 100 ug
EUR 356.15
Description: CXCL1 (Chemokine, CXC motif, Ligand 1), also called GRO1, SCYB1, GROA or MGSA, is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GRO?, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-?). In humans, this protein is encoded by the CXCL1 gene. The CXCL1 gene is mapped on 4q13.3. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. It is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis. Signaling through Cxcr2, Cxcl1 inhibited oligodendrocyte precursor migration. The migrational arrest was rapid, reversible, and concentration dependent, and it reflected enhanced cell/substrate interactions. White matter expression of Cxcl1 was temporospatially regulated. Others contribute to aggressive growth selectivity in the lung. Among the lung metastasis signature genes identified, several, including CXCL1, were functionally validated.

Mouse Growth-Regulated Alpha Protein (CXCL1) ELISA Kit

20-abx154438
  • EUR 6373.20
  • EUR 3404.40
  • EUR 801.60
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Growth-Regulated Alpha Protein (CXCL1) ELISA Kit

abx050043-96tests 96 tests
EUR 924

Mouse Growth-Regulated Alpha Protein (CXCL1) ELISA Kit

abx253956-96tests 96 tests
EUR 801.6

Mouse Growth-Regulated Alpha Protein (CXCL1) ELISA Kit

abx574645-96tests 96 tests
EUR 801.6

Mouse CXCL1/GROα(Growth Regulated Oncogene Alpha) ELISA Kit

EM0003 96T
EUR 571.5
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 9.375pg/ml

Cxcl1 Antibody, HRP conjugated

1-CSB-PA09217B0Rb
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Cxcl1. Recognizes Cxcl1 from Mouse. This antibody is HRP conjugated. Tested in the following application: ELISA

CXCL1 Antibody, HRP conjugated

1-CSB-PA06097B0Rb
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against CXCL1. Recognizes CXCL1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

Cxcl1 Antibody, FITC conjugated

1-CSB-PA09217C0Rb
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Cxcl1. Recognizes Cxcl1 from Mouse. This antibody is FITC conjugated. Tested in the following application: ELISA

CXCL1 Antibody, FITC conjugated

1-CSB-PA06097C0Rb
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against CXCL1. Recognizes CXCL1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
  • It was demonstrated that serum depletion had important affect on the attachment of HaCat cells onto tissue tradition plastic (TCP), porous substrates and cellulosic scaffolds, which was additional enhanced by the pre-seeded HDFs. The advanced constructions fashioned by the HDFs colonized inside the porous substrates and scaffolds not solely prevented the seeded HaCat cells from filtering via the open pores, but additionally acted as mobile substrates for HaCat cells to connect onto.

 

  • When mono-cultured on TCP, each HDFs and HaCat cells had been much less proliferative in medium with out serum than with serum. Nonetheless, each cell sorts had been efficiently co-cultured in 2D utilizing serum-free medium if the preliminary cell seeding density was greater than 80,000 cells/cm² (with 1:1 ratio).

 

  • Primarily based on the outcomes from 2D cultures, co-culture of each cell sorts on modular substrates with small open pores (125 μm) and cellulosic scaffolds with open pores of various sizes (50-300 µm) had been then carried out efficiently in serum-free medium. This examine demonstrated that the generic analysis platform had nice potential for in-depth understanding of HDFs and HaCat cells cultivated in serum-free medium, which may inform the processes for manufacturing pores and skin cells or tissues for scientific purposes.

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