gentur

The application of serum free medium in the research of isolating cancer stem cells

The isolation and identification of most cancers stem cells are the important thing factors in exploring traits of most cancers stem cells at current. A number of species of most cancers stem cells, for example, retinoblastoma tumor stem cells, most cancers stem cells of melanoma of choroid, breast most cancers stem cells, lung most cancers stem cells, colon most cancers stem cells, and many others., have been remoted and cultured efficiently by serum free medium whereas their organic capabilities and traits are acquired.

 

This overview concentrate on the appliance of serum free medium within the analysis of isolating most cancers stem cells, each ocular and common, by way of offering basis for additional analysis on ocular most cancers stem cells.

 

A generic analysis platform with 2-dimensional (2D) cell tradition know-how, a third-dimensional (3D) in vitro tissue mannequin, and a scaled-down cell tradition and imaging system in between, was utilized to deal with the problematic points related to using serum in pores and skin tissue engineering. Human dermal fibroblasts (HDFs) and immortalized keratinocytes (HaCat cells) mono- or co-cultured in serum or serum-free medium had been in contrast and analyzed by way of the platform.

 

gentur

  • It was demonstrated that serum depletion had important affect on the attachment of HaCat cells onto tissue tradition plastic (TCP), porous substrates and cellulosic scaffolds, which was additional enhanced by the pre-seeded HDFs. The advanced constructions fashioned by the HDFs colonized inside the porous substrates and scaffolds not solely prevented the seeded HaCat cells from filtering via the open pores, but additionally acted as mobile substrates for HaCat cells to connect onto.

 

  • When mono-cultured on TCP, each HDFs and HaCat cells had been much less proliferative in medium with out serum than with serum. Nonetheless, each cell sorts had been efficiently co-cultured in 2D utilizing serum-free medium if the preliminary cell seeding density was greater than 80,000 cells/cm² (with 1:1 ratio). Primarily based on the outcomes from 2D cultures, co-culture of each cell sorts on modular substrates with small open pores (125 μm) and cellulosic scaffolds with open pores of various sizes (50-300 µm) had been then carried out efficiently in serum-free medium.

Mouse LIF

MBS692107-5x001mg 5x0.01mg
EUR 1080

LIF,Mouse

E34M066M 5 μg
EUR 155

LIF, Mouse

HY-P7084 50ug
EUR 639.6

LIF, mouse

MO16102 100 ug
EUR 810

LIF, Mouse

MBS8575391-0005mg 0.005mg
EUR 245

LIF, Mouse

MBS8575391-5x0005mg 5x0.005mg
EUR 940

Mouse Anti-Human LIF Monoclonal Antibody

MBS592457-01mg 0.1mg
EUR 365

Mouse Anti-Human LIF Monoclonal Antibody

MBS592457-05mg 0.5mg
EUR 530

Mouse Anti-Human LIF Monoclonal Antibody

MBS592457-5x05mg 5x0.5mg
EUR 2140

Mouse Anti-Human LIF Monoclonal Antibody

MBS592458-01mg 0.1mg
EUR 365

Mouse Anti-Human LIF Monoclonal Antibody

MBS592458-05mg 0.5mg
EUR 530

Mouse Anti-Human LIF Monoclonal Antibody

MBS592458-5x05mg 5x0.5mg
EUR 2140

Mouse Anti-Human LIF Monoclonal Antibody

MBS592459-01mg 0.1mg
EUR 365

Mouse Anti-Human LIF Monoclonal Antibody

MBS592459-05mg 0.5mg
EUR 530

Mouse Anti-Human LIF Monoclonal Antibody

MBS592459-5x05mg 5x0.5mg
EUR 2140

Mouse LIF Protein

E40MOP1672 20ug
EUR 495

Mouse LIF Protein

E40MOP2026 20ug
EUR 495

Mouse LIF Protein

E40MOP2028 20ug
EUR 495

LIF, Mouse, Monoclonal Antibody, Mouse

MBS555093-01mg 0.1mg
EUR 745

LIF, Mouse, Monoclonal Antibody, Mouse

MBS555093-5x01mg 5x0.1mg
EUR 3210

LIF siRNA (Mouse)

MBS8224619-15nmol 15nmol
EUR 405

LIF siRNA (Mouse)

MBS8224619-30nmol 30nmol
EUR 565

LIF siRNA (Mouse)

MBS8224619-5x30nmol 5x30nmol
EUR 2450

Recombinant Mouse LIF

Z200195 10 µg
EUR 85
Description: LIF is a multifunctional secreted glycoprotein that exists in both soluble and matrix-bound forms. It displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation. It is now clear that LIF is related in both structure and mechanism of action to the interleukin IL-6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1. The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130.

Recombinant Mouse LIF

Z200197 100 µg
EUR 385
Description: LIF is a multifunctional secreted glycoprotein that exists in both soluble and matrix-bound forms. It displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation. It is now clear that LIF is related in both structure and mechanism of action to the interleukin IL-6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1. The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130.

Recombinant Mouse LIF

Z200199 1.0 mg
EUR 1200
Description: LIF is a multifunctional secreted glycoprotein that exists in both soluble and matrix-bound forms. It displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation (2). It is now clear that LIF is related in both structure and mechanism of action to the interleukin IL-6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1 (2). The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130.

Recombinant Mouse LIF

Z200485 100 µg
EUR 1500
Description: LIF is a multifunctional secreted glycoprotein that exists in both soluble and matrix-bound forms. It displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation (2). It is now clear that LIF is related in both structure and mechanism of action to the interleukin IL-6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1 (2). The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130.

Recombinant Mouse LIF

MBS7609292-005mg 0.05mg
EUR 405

Recombinant Mouse LIF

MBS7609292-02mg 0.2mg
EUR 760

Recombinant Mouse LIF

MBS7609292-1mg 1mg
EUR 2175

Recombinant Mouse LIF

MBS7609292-5x1mg 5x1mg
EUR 8410

Recombinant Mouse LIF

MBS4159936-001mg 0.01mg
EUR 230

Recombinant Mouse LIF

MBS4159936-01mg 0.1mg
EUR 560

Recombinant Mouse LIF

MBS4159936-1mg 1mg
EUR 1500

Recombinant Mouse LIF

MBS4159936-5x1mg 5x1mg
EUR 6500

Recombinant Mouse LIF

P1174-.005 5ug
EUR 108.8
Description: Peptides & Proteins|Recombinant Proteins

Recombinant Mouse LIF

P1174-.1 100ug
EUR 840.8
Description: Peptides & Proteins|Recombinant Proteins

Recombinant Mouse LIF

P1174-.5 500ug
EUR 1885.6
Description: Peptides & Proteins|Recombinant Proteins

Anti-Mouse LIF Antibody

103-M263 100 µg
EUR 399
Description: Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts.

Anti-Mouse LIF Antibody

103-PA05S 100 µg
EUR 126
Description: Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF.

Rabbit Anti-Mouse LIF

103-PA05 100ug
EUR 240

LIF (mouse), (recombinant)

MBS566073-1mL 1mL
EUR 1470

LIF (mouse), (recombinant)

MBS566073-5x1mL 5x1mL
EUR 6555

Mouse LIF ELISA Kit

E28L0650 96T
EUR 666.67

Mouse LIF ELISA Kit

EK5250 96 tests
EUR 599

Mouse Lif ELISA Kit

EF012949 96tests
EUR 566

Mouse LIF ELISA Kit

EML0014 96Tests
EUR 625.2

Mouse LIF shRNA Plasmid

20-abx971321
  • Ask for price
  • Ask for price
  • 150 µg
  • 300 µg

LIF, Human, Monoclonal Antibody, Mouse

MBS555094-01mg 0.1mg
EUR 745

LIF, Human, Monoclonal Antibody, Mouse

MBS555094-5x01mg 5x0.1mg
EUR 3210

Recombinant Mouse LIF protein

IMP-1308 10µg
EUR 360
Description: Recombinant Proteins

Mouse LIF Recombinant Protein

M30-007 10 µg
EUR 99.75
Description: Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. Recombinant mouse LIF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.86 kDa.

Mouse LIF Recombinant Protein

M30-008 50 µg
EUR 246.75
Description: Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. Recombinant mouse LIF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.86 kDa.

Recombinant Mouse LIF Protein

R01400-1 100ug/vial
EUR 352.8

Recombinant Mouse LIF protein

CD00323-5ug 5ug
EUR 176
Description: Leukemia inhibitory factor (LIF) is a member of Interleukin 6 family. This protein is mainly expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. LIF is used in mouse embryonic stem cell culture, because that removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. It is also used in phase II clinical trial, which can assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART). Mature mouse LIF shares 78 % a.a. sequence identity with Human LIF.

Recombinant Mouse LIF Protein

PKSM041100-100ug 100ug
EUR 739
Description: Mouse

Recombinant Mouse LIF Protein

PKSM041100-20ug 20ug
EUR 249
Description: Mouse

Recombinant Mouse LIF Protein

MBS8249390-001mg 0.01mg
EUR 230

Recombinant Mouse LIF Protein

MBS8249390-005mg 0.05mg
EUR 480

Recombinant Mouse LIF Protein

MBS8249390-05mg 0.5mg
EUR 2825

Recombinant Mouse LIF Protein

MBS8249390-5x05mg 5x0.5mg
EUR 12640

Recombinant Mouse LIF Protein

MBS1751002-01mg 0.1mg
EUR 315

Recombinant Mouse LIF Protein

MBS1751002-5x01mg 5x0.1mg
EUR 1270

Rat Monoclonal anti-mouse LIF

mAP-0122 100ug
EUR 250

Sheep Polyclonal anti-mouse LIF

mAP-5260 50ug
EUR 400

Anti-LIF Antibody, Mouse Monoclonal

MBS8107490-01mL 0.1mL
EUR 300

Anti-LIF Antibody, Mouse Monoclonal

MBS8107490-5x01mL 5x0.1mL
EUR 1200

anti- LIF antibody

LSMab09804 100ug
EUR 275

anti- LIF antibody

FNab09804 100µg
EUR 658.5
Description: Antibody raised against LIF
  • This examine demonstrated that the generic analysis platform had nice potential for in-depth understanding of HDFs and HaCat cells cultivated in serum-free medium, which may inform the processes for manufacturing pores and skin cells or tissues for scientific purposes.

Comparability of Human Dermal Fibroblasts and HaCat Cells Cultured in Medium with or with out Serum by way of a Generic Tissue Engineering Analysis Platform.

  • A generic analysis platform with 2-dimensional (2D) cell tradition know-how, a third-dimensional (3D) in vitro tissue mannequin, and a scaled-down cell tradition and imaging system in between, was utilized to deal with the problematic points related with using serum in pores and skin tissue engineering. Human dermal fibroblasts (HDFs) and immortalized keratinocytes (HaCat cells) mono- or co-cultured in serum or serum-free medium had been in contrast and analyzed by way of the platform.

Mouse KC (CXCL1)

MBS691637-5x002mg 5x0.02mg
EUR 1725

Anti-Mouse KC (CXCL1) Antibody

103-P23 100 µg
EUR 245.7
Description: The predicted amino acid sequence of the mouse KC gene product indicates KC is a member of the CXC subfamily of chemokines. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. In addition, the protein sequence of KC is approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs. KC mRNA is expressed in macrophages, endothelial cells, and fibroblasts. The rat chemokines CINC (cytokine-induced neutrophil chemoattractant)-1, CINC-2 alpha, CINC-2 beta and CINC-3 constitute a group of rat CXC chemokines that show significant sequence similarity to human GROs and mouse MIP-2 and KC.

Mouse CXCL1/KC ELISA KIT

E42EM-035 96T/48T Ask for price

Mouse CXCL1/KC ELISA Kit

E28L0076 96T
EUR 666.67

Mouse CXCL1/KC ELISA Kit

RK00038 96 Tests
EUR 280

Mouse CXCL1/KC ELISA Kit

MBS9135758-5x96Tests 5x96Tests
EUR 2615

Mouse CXCL1/KC ELISA Kit

MBS9135758-96Tests 96Tests
EUR 570

Mouse KC (CXCL1) Recombinant Protein

M10-027 20 µg
EUR 196.35
Description: All three isoforms of GRO or KC are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant murine KC is a 7.8 kDa protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2.

Mouse KC (CXCL1) Recombinant Protein

M10-027S 5 µg
EUR 92.4
Description: All three isoforms of GRO or KC are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant murine KC is a 7.8 kDa protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2.

Recombinant Mouse KC/CXCL1 protein

CD00330-5ug 5ug
EUR 176
Description: Mouse CXCL1, also known as KC, is belonging to the CXC chemokine family. It is encoded by the GRO gene now designated CXCL1. The gene for CXCL1 was initially discovered in mouse fibroblasts by plateletderived growth factor. KC is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. It is secreted by human melanoma cells, and also expressed by macrophages, neutrophils and epithelial cells. The functional receptor for CXCL1 has been identified as CXCR2. CXCL1 has chemotactic activity for neutrophils, and plays a role in inflammation and wound healing. Amino acid sequence of Mouse CXCL1 is approximately 60 % identical to the human CXCL1. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease.

Mouse GRO/KC (CXCL1), (Recombinant)

22060572-1 5 µg
EUR 146.42

Recombinant Mouse GRO/KC (CXCL1)

7-01858 5µg Ask for price

Recombinant Mouse GRO/KC (CXCL1)

7-01859 20µg Ask for price

Recombinant Mouse GRO/KC (CXCL1)

7-01860 1mg Ask for price

Recombinant Mouse GRO/KC (CXCL1)

MBS142042-0005mg 0.005mg
EUR 240

Recombinant Mouse GRO/KC (CXCL1)

MBS142042-002mg 0.02mg
EUR 310

Recombinant Mouse GRO/KC (CXCL1)

MBS142042-1mg 1mg
EUR 2880

Recombinant Mouse GRO/KC (CXCL1)

MBS142042-5x1mg 5x1mg
EUR 12630

GRO1/KC Mouse Recombinant (CXCL1)

rAP-0159 Inquiry Ask for price

Recombinant Mouse (E.Coli) GRO/KC (CXCL1)

RP-1037 5 ug
EUR 196.8

GRO1/KC Mouse Recombinant Protein (CXCL1)

PROTP12850-1 Regular: 20ug
EUR 380.4
Description: KC Mouse Recombinant also known as N51 and GRO1 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of approximately 8 kDa.;The GRO-1 is purified by proprietary chromatographic techniques.

GRO1/KC Mouse, GRO/KC (CXCL1) Mouse Recombinant Protein, His Tag

PROTP12850 Regular: 20ug
EUR 380.4
Description: GRO1/KC Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 97 amino acids (25-96 a.a.) and having a molecular mass of 10.5kDa.;GRO1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Recombinant Mouse Cxcl1/KC Protein, His Tag

E40MOP2119 20ug
EUR 495

KC (CXCL1) (AP)

MBS6484085-01mL 0.1mL
EUR 1100

KC (CXCL1) (AP)

MBS6484085-5x01mL 5x0.1mL
EUR 4810

KC (CXCL1) (PE)

MBS6484095-01mL 0.1mL
EUR 1100

KC (CXCL1) (PE)

MBS6484095-5x01mL 5x0.1mL
EUR 4810

KC (CXCL1) (APC)

MBS6484086-01mL 0.1mL
EUR 1100

KC (CXCL1) (APC)

MBS6484086-5x01mL 5x0.1mL
EUR 4810

KC (CXCL1) (HRP)

MBS6484089-01mL 0.1mL
EUR 1100

KC (CXCL1) (HRP)

MBS6484089-5x01mL 5x0.1mL
EUR 4810

GRO/KC (CXCL1) Mouse Recombinant, His Tag

rAP-0149 Inquiry Ask for price

Recombinant Mouse GRO/KC (CXCL1), His Tag

MBS144372-0005mg 0.005mg
EUR 240

Recombinant Mouse GRO/KC (CXCL1), His Tag

MBS144372-002mg 0.02mg
EUR 310

Recombinant Mouse GRO/KC (CXCL1), His Tag

MBS144372-1mg 1mg
EUR 3770

Recombinant Mouse GRO/KC (CXCL1), His Tag

MBS144372-5x1mg 5x1mg
EUR 16645

KC (CXCL1) (FITC)

MBS6484088-01mL 0.1mL
EUR 1100

KC (CXCL1) (FITC)

MBS6484088-5x01mL 5x0.1mL
EUR 4810

GRO-alpha, KC, CXCL1 (rMuKC), murine (mouse)

RC332-12 5ug
EUR 125.26

KC (CXCL1) (Biotin)

MBS6003763-005mg 0.05(mg
EUR 810

KC (CXCL1) (Biotin)

MBS6003763-5x005mg 5x0.05mg
EUR 3490

KC (CXCL1) (Biotin)

MBS6484087-01mL 0.1mL
EUR 1100

KC (CXCL1) (Biotin)

MBS6484087-5x01mL 5x0.1mL
EUR 4810

Sunlong Medical™ Mouse CXCL1/KC ELISA Kit

EL0012Mo-48T 48T
EUR 366

Sunlong Medical™ Mouse CXCL1/KC ELISA Kit

EL0012Mo-96T 96T
EUR 455

CXCL1 (Gro, KC) (AP)

MBS6470964-01mL 0.1mL
EUR 1115

CXCL1 (Gro, KC) (AP)

MBS6470964-5x01mL 5x0.1mL
EUR 4875

CXCL1 (Gro, KC) (PE)

MBS6470974-01mL 0.1mL
EUR 1115

CXCL1 (Gro, KC) (PE)

MBS6470974-5x01mL 5x0.1mL
EUR 4875

CXCL1 (Gro, KC) (APC)

MBS6470965-01mL 0.1mL
EUR 1115

CXCL1 (Gro, KC) (APC)

MBS6470965-5x01mL 5x0.1mL
EUR 4875

CXCL1 (Gro, KC) (HRP)

MBS6470968-01mL 0.1mL
EUR 1115

CXCL1 (Gro, KC) (HRP)

MBS6470968-5x01mL 5x0.1mL
EUR 4875

Rat GRO/KC (CXCL1)

MBS691984-0005mg 0.005mg
EUR 300

Rat GRO/KC (CXCL1)

MBS691984-0025mg 0.025mg
EUR 450

Rat GRO/KC (CXCL1)

MBS691984-5x0025mg 5x0.025mg
EUR 1725

CXCL1 (Gro, KC) (FITC)

MBS6470967-01mL 0.1mL
EUR 1115

CXCL1 (Gro, KC) (FITC)

MBS6470967-5x01mL 5x0.1mL
EUR 4875

CXCL1 (Gro, KC) (Biotin)

MBS6470966-01mL 0.1mL
EUR 1115

CXCL1 (Gro, KC) (Biotin)

MBS6470966-5x01mL 5x0.1mL
EUR 4875

GRO / KC (CXCL1) Protein

20-abx261870
  • Ask for price
  • Ask for price
  • Ask for price
  • 1 mg
  • 20 ug
  • 5 ug

GRO / KC (CXCL1) Protein

20-abx260684
  • Ask for price
  • Ask for price
  • Ask for price
  • 1 mg
  • 20 ug
  • 5 ug

GRO / KC (CXCL1) Protein

abx261870-10mg 10 mg
EUR 325

GRO / KC (CXCL1) Protein

abx261870-25mg 25 mg
EUR 4625

GRO / KC (CXCL1) Protein

abx261870-5mg 5 mg
EUR 225

GRO / KC (CXCL1) Protein

abx260684-10g 10 µg
EUR 325

GRO / KC (CXCL1) Protein

abx260684-2g 2 µg
EUR 225

KC (CXCL1) (MaxLight 405)

MBS6484090-01mL 0.1mL
EUR 1100

KC (CXCL1) (MaxLight 405)

MBS6484090-5x01mL 5x0.1mL
EUR 4810

KC (CXCL1) (MaxLight 490)

MBS6484091-01mL 0.1mL
EUR 1100

KC (CXCL1) (MaxLight 490)

MBS6484091-5x01mL 5x0.1mL
EUR 4810

KC (CXCL1) (MaxLight 550)

MBS6484092-01mL 0.1mL
EUR 1100

KC (CXCL1) (MaxLight 550)

MBS6484092-5x01mL 5x0.1mL
EUR 4810

KC (CXCL1) (MaxLight 650)

MBS6484093-01mL 0.1mL
EUR 1100

KC (CXCL1) (MaxLight 650)

MBS6484093-5x01mL 5x0.1mL
EUR 4810

KC (CXCL1) (MaxLight 750)

MBS6484094-01mL 0.1mL
EUR 1100

KC (CXCL1) (MaxLight 750)

MBS6484094-5x01mL 5x0.1mL
EUR 4810

RABBIT ANTI MOUSE CXCL1

MBS221269-01mg 0.1mg
EUR 635

RABBIT ANTI MOUSE CXCL1

MBS221269-5x01mg 5x0.1mg
EUR 2695

Mouse CXC-chemokine ligand 1(CXCL1/KC)ELISA Kit

YLA0122MO-48T 48T Ask for price

Mouse CXC-chemokine ligand 1(CXCL1/KC)ELISA Kit

YLA0122MO-96T 96T Ask for price

Mouse CXC-chemokine ligand 1,CXCL1;KC ELISA KIT

E1520Mo-1096T 10*96T
EUR 4122

Mouse CXC-chemokine ligand 1,CXCL1;KC ELISA KIT

E1520Mo-48wells 48 wells
EUR 300

Mouse CXC-chemokine ligand 1,CXCL1;KC ELISA KIT

E1520Mo-596T 5*96T
EUR 2061

Mouse CXC-chemokine ligand 1,CXCL1;KC ELISA KIT

E1520Mo-96wells 96 wells
EUR 458
  • It was demonstrated that serum depletion had important affect on the attachment of HaCat cells onto tissue tradition plastic (TCP), porous substrates and cellulosic scaffolds, which was additional enhanced by the pre-seeded HDFs. The advanced constructions fashioned by the HDFs colonized inside the porous substrates and scaffolds not solely prevented the seeded HaCat cells from filtering via the open pores, but additionally acted as mobile substrates for HaCat cells to connect onto.

 

  • When mono-cultured on TCP, each HDFs and HaCat cells had been much less proliferative in medium with out serum than with serum. Nonetheless, each cell sorts had been efficiently co-cultured in 2D utilizing serum-free medium if the preliminary cell seeding density was greater than 80,000 cells/cm² (with 1:1 ratio).

 

  • Primarily based on the outcomes from 2D cultures, co-culture of each cell sorts on modular substrates with small open pores (125 μm) and cellulosic scaffolds with open pores of various sizes (50-300 µm) had been then carried out efficiently in serum-free medium. This examine demonstrated that the generic analysis platform had nice potential for in-depth understanding of HDFs and HaCat cells cultivated in serum-free medium, which may inform the processes for manufacturing pores and skin cells or tissues for scientific purposes.
Gina

Leave a Comment

Your email address will not be published.