The isolation and identification of most cancers stem cells are the important thing factors in exploring traits of most cancers stem cells at current. A number of species of most cancers stem cells, for example, retinoblastoma tumor stem cells, most cancers stem cells of melanoma of choroid, breast most cancers stem cells, lung most cancers stem cells, colon most cancers stem cells, and many others., have been remoted and cultured efficiently by serum free medium whereas their organic capabilities and traits are acquired.
This overview concentrate on the appliance of serum free medium within the analysis of isolating most cancers stem cells, each ocular and common, by way of offering basis for additional analysis on ocular most cancers stem cells.
A generic analysis platform with 2-dimensional (2D) cell tradition know-how, a third-dimensional (3D) in vitro tissue mannequin, and a scaled-down cell tradition and imaging system in between, was utilized to deal with the problematic points related to using serum in pores and skin tissue engineering. Human dermal fibroblasts (HDFs) and immortalized keratinocytes (HaCat cells) mono- or co-cultured in serum or serum-free medium had been in contrast and analyzed by way of the platform.
- It was demonstrated that serum depletion had important affect on the attachment of HaCat cells onto tissue tradition plastic (TCP), porous substrates and cellulosic scaffolds, which was additional enhanced by the pre-seeded HDFs. The advanced constructions fashioned by the HDFs colonized inside the porous substrates and scaffolds not solely prevented the seeded HaCat cells from filtering via the open pores, but additionally acted as mobile substrates for HaCat cells to connect onto.
- When mono-cultured on TCP, each HDFs and HaCat cells had been much less proliferative in medium with out serum than with serum. Nonetheless, each cell sorts had been efficiently co-cultured in 2D utilizing serum-free medium if the preliminary cell seeding density was greater than 80,000 cells/cm² (with 1:1 ratio). Primarily based on the outcomes from 2D cultures, co-culture of each cell sorts on modular substrates with small open pores (125 μm) and cellulosic scaffolds with open pores of various sizes (50-300 µm) had been then carried out efficiently in serum-free medium.
Mouse LIF |
MBS692107-5x001mg |
MyBiosource |
5x0.01mg |
EUR 1080 |
LIF,Mouse |
E34M066M |
EnoGene |
5 μg |
EUR 155 |
LIF, Mouse |
MBS8575391-0005mg |
MyBiosource |
0.005mg |
EUR 245 |
LIF, Mouse |
MBS8575391-5x0005mg |
MyBiosource |
5x0.005mg |
EUR 940 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592457-01mg |
MyBiosource |
0.1mg |
EUR 365 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592457-05mg |
MyBiosource |
0.5mg |
EUR 530 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592457-5x05mg |
MyBiosource |
5x0.5mg |
EUR 2140 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592458-01mg |
MyBiosource |
0.1mg |
EUR 365 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592458-05mg |
MyBiosource |
0.5mg |
EUR 530 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592458-5x05mg |
MyBiosource |
5x0.5mg |
EUR 2140 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592459-01mg |
MyBiosource |
0.1mg |
EUR 365 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592459-05mg |
MyBiosource |
0.5mg |
EUR 530 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592459-5x05mg |
MyBiosource |
5x0.5mg |
EUR 2140 |
Mouse LIF Protein |
E40MOP1672 |
EnoGene |
20ug |
EUR 495 |
Mouse LIF Protein |
E40MOP2026 |
EnoGene |
20ug |
EUR 495 |
Mouse LIF Protein |
E40MOP2028 |
EnoGene |
20ug |
EUR 495 |
LIF, Mouse, Monoclonal Antibody, Mouse |
MBS555093-01mg |
MyBiosource |
0.1mg |
EUR 745 |
LIF, Mouse, Monoclonal Antibody, Mouse |
MBS555093-5x01mg |
MyBiosource |
5x0.1mg |
EUR 3210 |
LIF siRNA (Mouse) |
MBS8224619-15nmol |
MyBiosource |
15nmol |
EUR 405 |
LIF siRNA (Mouse) |
MBS8224619-30nmol |
MyBiosource |
30nmol |
EUR 565 |
LIF siRNA (Mouse) |
MBS8224619-5x30nmol |
MyBiosource |
5x30nmol |
EUR 2450 |
Recombinant Mouse LIF |
Z200195 |
ABM |
10 µg |
EUR 85 |
Description: LIF is a multifunctional secreted glycoprotein that exists in both soluble and matrix-bound forms. It displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation. It is now clear that LIF is related in both structure and mechanism of action to the interleukin IL-6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1. The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130. |
Recombinant Mouse LIF |
Z200197 |
ABM |
100 µg |
EUR 385 |
Description: LIF is a multifunctional secreted glycoprotein that exists in both soluble and matrix-bound forms. It displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation. It is now clear that LIF is related in both structure and mechanism of action to the interleukin IL-6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1. The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130. |
Recombinant Mouse LIF |
Z200199 |
ABM |
1.0 mg |
EUR 1200 |
Description: LIF is a multifunctional secreted glycoprotein that exists in both soluble and matrix-bound forms. It displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation (2). It is now clear that LIF is related in both structure and mechanism of action to the interleukin IL-6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1 (2). The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130. |
Recombinant Mouse LIF |
Z200485 |
ABM |
100 µg |
EUR 1500 |
Description: LIF is a multifunctional secreted glycoprotein that exists in both soluble and matrix-bound forms. It displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation (2). It is now clear that LIF is related in both structure and mechanism of action to the interleukin IL-6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1 (2). The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130. |
Recombinant Mouse LIF |
MBS7609292-005mg |
MyBiosource |
0.05mg |
EUR 405 |
Recombinant Mouse LIF |
MBS7609292-02mg |
MyBiosource |
0.2mg |
EUR 760 |
Recombinant Mouse LIF |
MBS7609292-1mg |
MyBiosource |
1mg |
EUR 2175 |
Recombinant Mouse LIF |
MBS7609292-5x1mg |
MyBiosource |
5x1mg |
EUR 8410 |
Recombinant Mouse LIF |
MBS4159936-001mg |
MyBiosource |
0.01mg |
EUR 230 |
Recombinant Mouse LIF |
MBS4159936-01mg |
MyBiosource |
0.1mg |
EUR 560 |
Recombinant Mouse LIF |
MBS4159936-1mg |
MyBiosource |
1mg |
EUR 1500 |
Recombinant Mouse LIF |
MBS4159936-5x1mg |
MyBiosource |
5x1mg |
EUR 6500 |
Recombinant Mouse LIF |
P1174-.005 |
ApexBio |
5ug |
EUR 108.8 |
Description: Peptides & Proteins|Recombinant Proteins |
Recombinant Mouse LIF |
P1174-.1 |
ApexBio |
100ug |
EUR 840.8 |
Description: Peptides & Proteins|Recombinant Proteins |
Recombinant Mouse LIF |
P1174-.5 |
ApexBio |
500ug |
EUR 1885.6 |
Description: Peptides & Proteins|Recombinant Proteins |
Anti-Mouse LIF Antibody |
103-M263 |
ReliaTech |
100 µg |
EUR 399 |
Description: Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. |
Anti-Mouse LIF Antibody |
103-PA05S |
ReliaTech |
100 µg |
EUR 126 |
Description: Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. |
LIF (mouse), (recombinant) |
MBS566073-1mL |
MyBiosource |
1mL |
EUR 1470 |
LIF (mouse), (recombinant) |
MBS566073-5x1mL |
MyBiosource |
5x1mL |
EUR 6555 |
Mouse LIF ELISA Kit |
E28L0650 |
EnoGene |
96T |
EUR 666.67 |
Mouse LIF ELISA Kit |
EK5250 |
SAB |
96 tests |
EUR 599 |
|
Mouse LIF ELISA Kit |
EML0014 |
Abclonal |
96Tests |
EUR 625.2 |
Mouse LIF shRNA Plasmid |
20-abx971321 |
Abbexa |
-
Ask for price
-
Ask for price
|
|
|
LIF, Human, Monoclonal Antibody, Mouse |
MBS555094-01mg |
MyBiosource |
0.1mg |
EUR 745 |
LIF, Human, Monoclonal Antibody, Mouse |
MBS555094-5x01mg |
MyBiosource |
5x0.1mg |
EUR 3210 |
Recombinant Mouse LIF protein |
IMP-1308 |
Creative BioMart |
10µg |
EUR 360 |
|
Description: Recombinant Proteins |
Mouse LIF Recombinant Protein |
M30-007 |
ReliaTech |
10 µg |
EUR 99.75 |
Description: Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. Recombinant mouse LIF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.86 kDa. |
Mouse LIF Recombinant Protein |
M30-008 |
ReliaTech |
50 µg |
EUR 246.75 |
Description: Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. Recombinant mouse LIF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.86 kDa. |
Recombinant Mouse LIF Protein |
R01400-1 |
BosterBio |
100ug/vial |
EUR 352.8 |
Recombinant Mouse LIF protein |
CD00323-5ug |
Jiaxing Korain Biotech Ltd (BT Labs) |
5ug |
EUR 176 |
|
Description: Leukemia inhibitory factor (LIF) is a member of Interleukin 6 family. This protein is mainly expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. LIF is used in mouse embryonic stem cell culture, because that removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. It is also used in phase II clinical trial, which can assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART). Mature mouse LIF shares 78 % a.a. sequence identity with Human LIF. |
Recombinant Mouse LIF Protein |
PKSM041100-100ug |
Elabscience Biotech |
100ug |
EUR 739 |
|
Description: Mouse |
Recombinant Mouse LIF Protein |
PKSM041100-20ug |
Elabscience Biotech |
20ug |
EUR 249 |
|
Description: Mouse |
Recombinant Mouse LIF Protein |
MBS8249390-001mg |
MyBiosource |
0.01mg |
EUR 230 |
Recombinant Mouse LIF Protein |
MBS8249390-005mg |
MyBiosource |
0.05mg |
EUR 480 |
Recombinant Mouse LIF Protein |
MBS8249390-05mg |
MyBiosource |
0.5mg |
EUR 2825 |
Recombinant Mouse LIF Protein |
MBS8249390-5x05mg |
MyBiosource |
5x0.5mg |
EUR 12640 |
Recombinant Mouse LIF Protein |
MBS1751002-01mg |
MyBiosource |
0.1mg |
EUR 315 |
Recombinant Mouse LIF Protein |
MBS1751002-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1270 |
Anti-LIF Antibody, Mouse Monoclonal |
MBS8107490-01mL |
MyBiosource |
0.1mL |
EUR 300 |
Anti-LIF Antibody, Mouse Monoclonal |
MBS8107490-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1200 |
anti- LIF antibody |
FNab09804 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against LIF |
- This examine demonstrated that the generic analysis platform had nice potential for in-depth understanding of HDFs and HaCat cells cultivated in serum-free medium, which may inform the processes for manufacturing pores and skin cells or tissues for scientific purposes.
Comparability of Human Dermal Fibroblasts and HaCat Cells Cultured in Medium with or with out Serum by way of a Generic Tissue Engineering Analysis Platform.
- A generic analysis platform with 2-dimensional (2D) cell tradition know-how, a third-dimensional (3D) in vitro tissue mannequin, and a scaled-down cell tradition and imaging system in between, was utilized to deal with the problematic points related with using serum in pores and skin tissue engineering. Human dermal fibroblasts (HDFs) and immortalized keratinocytes (HaCat cells) mono- or co-cultured in serum or serum-free medium had been in contrast and analyzed by way of the platform.
Mouse KC (CXCL1) |
MBS691637-5x002mg |
MyBiosource |
5x0.02mg |
EUR 1725 |
Anti-Mouse KC (CXCL1) Antibody |
103-P23 |
ReliaTech |
100 µg |
EUR 245.7 |
Description: The predicted amino acid sequence of the mouse KC gene product indicates KC is a member of the CXC subfamily of chemokines. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. In addition, the protein sequence of KC is approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs. KC mRNA is expressed in macrophages, endothelial cells, and fibroblasts. The rat chemokines CINC (cytokine-induced neutrophil chemoattractant)-1, CINC-2 alpha, CINC-2 beta and CINC-3 constitute a group of rat CXC chemokines that show significant sequence similarity to human GROs and mouse MIP-2 and KC. |
Mouse CXCL1/KC ELISA KIT |
E42EM-035 |
EnoGene |
96T/48T |
Ask for price |
Mouse CXCL1/KC ELISA Kit |
E28L0076 |
EnoGene |
96T |
EUR 666.67 |
Mouse CXCL1/KC ELISA Kit |
RK00038 |
Abclonal |
96 Tests |
EUR 280 |
Mouse CXCL1/KC ELISA Kit |
MBS9135758-5x96Tests |
MyBiosource |
5x96Tests |
EUR 2615 |
Mouse CXCL1/KC ELISA Kit |
MBS9135758-96Tests |
MyBiosource |
96Tests |
EUR 570 |
Mouse KC (CXCL1) Recombinant Protein |
M10-027 |
ReliaTech |
20 µg |
EUR 196.35 |
Description: All three isoforms of GRO or KC are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant murine KC is a 7.8 kDa protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2. |
Mouse KC (CXCL1) Recombinant Protein |
M10-027S |
ReliaTech |
5 µg |
EUR 92.4 |
Description: All three isoforms of GRO or KC are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant murine KC is a 7.8 kDa protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2. |
Recombinant Mouse KC/CXCL1 protein |
CD00330-5ug |
Jiaxing Korain Biotech Ltd (BT Labs) |
5ug |
EUR 176 |
|
Description: Mouse CXCL1, also known as KC, is belonging to the CXC chemokine family. It is encoded by the GRO gene now designated CXCL1. The gene for CXCL1 was initially discovered in mouse fibroblasts by plateletderived growth factor. KC is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. It is secreted by human melanoma cells, and also expressed by macrophages, neutrophils and epithelial cells. The functional receptor for CXCL1 has been identified as CXCR2. CXCL1 has chemotactic activity for neutrophils, and plays a role in inflammation and wound healing. Amino acid sequence of Mouse CXCL1 is approximately 60 % identical to the human CXCL1. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease. |
Mouse GRO/KC (CXCL1), (Recombinant) |
22060572-1 |
Glycomatrix |
5 µg |
EUR 146.42 |
Recombinant Mouse GRO/KC (CXCL1) |
7-01858 |
CHI Scientific |
5µg |
Ask for price |
Recombinant Mouse GRO/KC (CXCL1) |
7-01859 |
CHI Scientific |
20µg |
Ask for price |
Recombinant Mouse GRO/KC (CXCL1) |
7-01860 |
CHI Scientific |
1mg |
Ask for price |
Recombinant Mouse GRO/KC (CXCL1) |
MBS142042-0005mg |
MyBiosource |
0.005mg |
EUR 240 |
Recombinant Mouse GRO/KC (CXCL1) |
MBS142042-002mg |
MyBiosource |
0.02mg |
EUR 310 |
Recombinant Mouse GRO/KC (CXCL1) |
MBS142042-1mg |
MyBiosource |
1mg |
EUR 2880 |
Recombinant Mouse GRO/KC (CXCL1) |
MBS142042-5x1mg |
MyBiosource |
5x1mg |
EUR 12630 |
GRO1/KC Mouse Recombinant (CXCL1) |
rAP-0159 |
Angio Proteomie |
Inquiry |
Ask for price |
GRO1/KC Mouse Recombinant Protein (CXCL1) |
PROTP12850-1 |
BosterBio |
Regular: 20ug |
EUR 380.4 |
Description: KC Mouse Recombinant also known as N51 and GRO1 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of approximately 8 kDa.;The GRO-1 is purified by proprietary chromatographic techniques. |
GRO1/KC Mouse, GRO/KC (CXCL1) Mouse Recombinant Protein, His Tag |
PROTP12850 |
BosterBio |
Regular: 20ug |
EUR 380.4 |
Description: GRO1/KC Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 97 amino acids (25-96 a.a.) and having a molecular mass of 10.5kDa.;GRO1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
Recombinant Mouse Cxcl1/KC Protein, His Tag |
E40MOP2119 |
EnoGene |
20ug |
EUR 495 |
KC (CXCL1) (AP) |
MBS6484085-01mL |
MyBiosource |
0.1mL |
EUR 1100 |
KC (CXCL1) (AP) |
MBS6484085-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4810 |
KC (CXCL1) (PE) |
MBS6484095-01mL |
MyBiosource |
0.1mL |
EUR 1100 |
KC (CXCL1) (PE) |
MBS6484095-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4810 |
KC (CXCL1) (APC) |
MBS6484086-01mL |
MyBiosource |
0.1mL |
EUR 1100 |
KC (CXCL1) (APC) |
MBS6484086-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4810 |
KC (CXCL1) (HRP) |
MBS6484089-01mL |
MyBiosource |
0.1mL |
EUR 1100 |
KC (CXCL1) (HRP) |
MBS6484089-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4810 |
GRO/KC (CXCL1) Mouse Recombinant, His Tag |
rAP-0149 |
Angio Proteomie |
Inquiry |
Ask for price |
Recombinant Mouse GRO/KC (CXCL1), His Tag |
MBS144372-0005mg |
MyBiosource |
0.005mg |
EUR 240 |
Recombinant Mouse GRO/KC (CXCL1), His Tag |
MBS144372-002mg |
MyBiosource |
0.02mg |
EUR 310 |
Recombinant Mouse GRO/KC (CXCL1), His Tag |
MBS144372-1mg |
MyBiosource |
1mg |
EUR 3770 |
Recombinant Mouse GRO/KC (CXCL1), His Tag |
MBS144372-5x1mg |
MyBiosource |
5x1mg |
EUR 16645 |
KC (CXCL1) (FITC) |
MBS6484088-01mL |
MyBiosource |
0.1mL |
EUR 1100 |
KC (CXCL1) (FITC) |
MBS6484088-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4810 |
GRO-alpha, KC, CXCL1 (rMuKC), murine (mouse) |
RC332-12 |
Bio Basic |
5ug |
EUR 125.26 |
|
KC (CXCL1) (Biotin) |
MBS6003763-005mg |
MyBiosource |
0.05(mg |
EUR 810 |
KC (CXCL1) (Biotin) |
MBS6003763-5x005mg |
MyBiosource |
5x0.05mg |
EUR 3490 |
KC (CXCL1) (Biotin) |
MBS6484087-01mL |
MyBiosource |
0.1mL |
EUR 1100 |
KC (CXCL1) (Biotin) |
MBS6484087-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4810 |
Sunlong Medicalâ„¢ Mouse CXCL1/KC ELISA Kit |
EL0012Mo-48T |
Sunlong |
48T |
EUR 366 |
Sunlong Medicalâ„¢ Mouse CXCL1/KC ELISA Kit |
EL0012Mo-96T |
Sunlong |
96T |
EUR 455 |
CXCL1 (Gro, KC) (AP) |
MBS6470964-01mL |
MyBiosource |
0.1mL |
EUR 1115 |
CXCL1 (Gro, KC) (AP) |
MBS6470964-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4875 |
CXCL1 (Gro, KC) (PE) |
MBS6470974-01mL |
MyBiosource |
0.1mL |
EUR 1115 |
CXCL1 (Gro, KC) (PE) |
MBS6470974-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4875 |
CXCL1 (Gro, KC) (APC) |
MBS6470965-01mL |
MyBiosource |
0.1mL |
EUR 1115 |
CXCL1 (Gro, KC) (APC) |
MBS6470965-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4875 |
CXCL1 (Gro, KC) (HRP) |
MBS6470968-01mL |
MyBiosource |
0.1mL |
EUR 1115 |
CXCL1 (Gro, KC) (HRP) |
MBS6470968-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4875 |
Rat GRO/KC (CXCL1) |
MBS691984-0005mg |
MyBiosource |
0.005mg |
EUR 300 |
Rat GRO/KC (CXCL1) |
MBS691984-0025mg |
MyBiosource |
0.025mg |
EUR 450 |
Rat GRO/KC (CXCL1) |
MBS691984-5x0025mg |
MyBiosource |
5x0.025mg |
EUR 1725 |
CXCL1 (Gro, KC) (FITC) |
MBS6470967-01mL |
MyBiosource |
0.1mL |
EUR 1115 |
CXCL1 (Gro, KC) (FITC) |
MBS6470967-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4875 |
CXCL1 (Gro, KC) (Biotin) |
MBS6470966-01mL |
MyBiosource |
0.1mL |
EUR 1115 |
CXCL1 (Gro, KC) (Biotin) |
MBS6470966-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4875 |
GRO / KC (CXCL1) Protein |
20-abx261870 |
Abbexa |
-
Ask for price
-
Ask for price
-
Ask for price
|
|
|
GRO / KC (CXCL1) Protein |
20-abx260684 |
Abbexa |
-
Ask for price
-
Ask for price
-
Ask for price
|
|
|
GRO / KC (CXCL1) Protein |
abx261870-10mg |
Abbexa |
10 mg |
EUR 325 |
GRO / KC (CXCL1) Protein |
abx261870-25mg |
Abbexa |
25 mg |
EUR 4625 |
GRO / KC (CXCL1) Protein |
abx261870-5mg |
Abbexa |
5 mg |
EUR 225 |
GRO / KC (CXCL1) Protein |
abx260684-10g |
Abbexa |
10 µg |
EUR 325 |
GRO / KC (CXCL1) Protein |
abx260684-2g |
Abbexa |
2 µg |
EUR 225 |
KC (CXCL1) (MaxLight 405) |
MBS6484090-01mL |
MyBiosource |
0.1mL |
EUR 1100 |
KC (CXCL1) (MaxLight 405) |
MBS6484090-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4810 |
KC (CXCL1) (MaxLight 490) |
MBS6484091-01mL |
MyBiosource |
0.1mL |
EUR 1100 |
KC (CXCL1) (MaxLight 490) |
MBS6484091-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4810 |
KC (CXCL1) (MaxLight 550) |
MBS6484092-01mL |
MyBiosource |
0.1mL |
EUR 1100 |
KC (CXCL1) (MaxLight 550) |
MBS6484092-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4810 |
KC (CXCL1) (MaxLight 650) |
MBS6484093-01mL |
MyBiosource |
0.1mL |
EUR 1100 |
KC (CXCL1) (MaxLight 650) |
MBS6484093-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4810 |
KC (CXCL1) (MaxLight 750) |
MBS6484094-01mL |
MyBiosource |
0.1mL |
EUR 1100 |
KC (CXCL1) (MaxLight 750) |
MBS6484094-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4810 |
RABBIT ANTI MOUSE CXCL1 |
MBS221269-01mg |
MyBiosource |
0.1mg |
EUR 635 |
RABBIT ANTI MOUSE CXCL1 |
MBS221269-5x01mg |
MyBiosource |
5x0.1mg |
EUR 2695 |
Mouse CXC-chemokine ligand 1(CXCL1/KC)ELISA Kit |
YLA0122MO-48T |
Shanghai YL Biotech |
48T |
Ask for price |
Mouse CXC-chemokine ligand 1(CXCL1/KC)ELISA Kit |
YLA0122MO-96T |
Shanghai YL Biotech |
96T |
Ask for price |
- It was demonstrated that serum depletion had important affect on the attachment of HaCat cells onto tissue tradition plastic (TCP), porous substrates and cellulosic scaffolds, which was additional enhanced by the pre-seeded HDFs. The advanced constructions fashioned by the HDFs colonized inside the porous substrates and scaffolds not solely prevented the seeded HaCat cells from filtering via the open pores, but additionally acted as mobile substrates for HaCat cells to connect onto.
- When mono-cultured on TCP, each HDFs and HaCat cells had been much less proliferative in medium with out serum than with serum. Nonetheless, each cell sorts had been efficiently co-cultured in 2D utilizing serum-free medium if the preliminary cell seeding density was greater than 80,000 cells/cm² (with 1:1 ratio).
- Primarily based on the outcomes from 2D cultures, co-culture of each cell sorts on modular substrates with small open pores (125 μm) and cellulosic scaffolds with open pores of various sizes (50-300 µm) had been then carried out efficiently in serum-free medium. This examine demonstrated that the generic analysis platform had nice potential for in-depth understanding of HDFs and HaCat cells cultivated in serum-free medium, which may inform the processes for manufacturing pores and skin cells or tissues for scientific purposes.