The isolation and identification of most cancers stem cells are the important thing factors in exploring traits of most cancers stem cells at current. A number of species of most cancers stem cells, for example, retinoblastoma tumor stem cells, most cancers stem cells of melanoma of choroid, breast most cancers stem cells, lung most cancers stem cells, colon most cancers stem cells, and many others., have been remoted and cultured efficiently by serum free medium whereas their organic capabilities and traits are acquired.
This overview concentrate on the appliance of serum free medium within the analysis of isolating most cancers stem cells, each ocular and common, by way of offering basis for additional analysis on ocular most cancers stem cells.
A generic analysis platform with 2-dimensional (2D) cell tradition know-how, a third-dimensional (3D) in vitro tissue mannequin, and a scaled-down cell tradition and imaging system in between, was utilized to deal with the problematic points related to using serum in pores and skin tissue engineering. Human dermal fibroblasts (HDFs) and immortalized keratinocytes (HaCat cells) mono- or co-cultured in serum or serum-free medium had been in contrast and analyzed by way of the platform.

- It was demonstrated that serum depletion had important affect on the attachment of HaCat cells onto tissue tradition plastic (TCP), porous substrates and cellulosic scaffolds, which was additional enhanced by the pre-seeded HDFs. The advanced constructions fashioned by the HDFs colonized inside the porous substrates and scaffolds not solely prevented the seeded HaCat cells from filtering via the open pores, but additionally acted as mobile substrates for HaCat cells to connect onto.
- When mono-cultured on TCP, each HDFs and HaCat cells had been much less proliferative in medium with out serum than with serum. Nonetheless, each cell sorts had been efficiently co-cultured in 2D utilizing serum-free medium if the preliminary cell seeding density was greater than 80,000 cells/cm² (with 1:1 ratio). Primarily based on the outcomes from 2D cultures, co-culture of each cell sorts on modular substrates with small open pores (125 μm) and cellulosic scaffolds with open pores of various sizes (50-300 µm) had been then carried out efficiently in serum-free medium.
Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 613.2 |
Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 850.8 |
anti-LIF |
YF-PA12952 |
Abfrontier |
50 ug |
EUR 435.6 |
Description: Mouse polyclonal to LIF |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-b-48T |
DL Develop |
48T |
EUR 656.4 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-b-96T |
DL Develop |
96T |
EUR 858 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-c-48T |
DL Develop |
48T |
EUR 632.4 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-c-96T |
DL Develop |
96T |
EUR 825.6 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-Hu-48T |
DL Develop |
48T |
EUR 456 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-Hu-96T |
DL Develop |
96T |
EUR 582 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-p-48T |
DL Develop |
48T |
EUR 656.4 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-p-96T |
DL Develop |
96T |
EUR 858 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-Ra-48T |
DL Develop |
48T |
EUR 609.6 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-Ra-96T |
DL Develop |
96T |
EUR 793.2 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 666 |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 925.2 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-c-48Tests |
Reddot Biotech |
48 Tests |
EUR 639.6 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-c-96Tests |
Reddot Biotech |
96 Tests |
EUR 888 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 440.4 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 602.4 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-p-48Tests |
Reddot Biotech |
48 Tests |
EUR 666 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-p-96Tests |
Reddot Biotech |
96 Tests |
EUR 925.2 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 613.2 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 850.8 |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 696 |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 968.4 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-c-48Tests |
Reddot Biotech |
48 Tests |
EUR 668.4 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-c-96Tests |
Reddot Biotech |
96 Tests |
EUR 928.8 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 459.6 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 630 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-p-48Tests |
Reddot Biotech |
48 Tests |
EUR 696 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-p-96Tests |
Reddot Biotech |
96 Tests |
EUR 968.4 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 640.8 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 890.4 |
Rabbit Polyclonal antibody Anti-CRBN |
Anti-CRBN |
ImmunoStep |
50 µg |
EUR 418.8 |
Anti-LIF Antibody |
A01400 |
BosterBio |
100ug/vial |
EUR 352.8 |
Anti-LIF Antibody |
PA1562 |
BosterBio |
100ug/vial |
EUR 352.8 |
anti- LIF antibody |
FNab09804 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against LIF |
Anti-LIF Antibody |
PB9036 |
BosterBio |
100ug/vial |
EUR 400.8 |
Anti-LIF antibody |
STJ29846 |
St John's Laboratory |
100 µl |
EUR 332.4 |
Description: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
Anti-LIF antibody |
STJ96472 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to LIF. |
Mouse LIF shRNA Plasmid |
20-abx971321 |
Abbexa |
|
|
|
Mouse LIF ELISA Kit |
EML0014 |
Abclonal |
96Tests |
EUR 625.2 |
Recombinant Mouse LIF Protein |
R01400-1 |
BosterBio |
100ug/vial |
EUR 352.8 |
Polyclonal Goat anti-GST α-form |
GST-ANTI-1 |
Detroit R&D |
50 uL |
EUR 336 |
Polyclonal Goat anti-GST μ-form |
GST-ANTI-2 |
Detroit R&D |
50 uL |
EUR 336 |
Polyclonal Goat anti-GST p-form |
GST-ANTI-3 |
Detroit R&D |
50 uL |
EUR 336 |
LIF Antibody |
1-CSB-PA198481 |
Cusabio |
|
|
|
Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100 |
LIF Antibody |
1-CSB-PA004761 |
Cusabio |
|
|
|
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000 |
LIF Antibody |
1-CSB-PA012928ESR2HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: ELISA |
LIF antibody |
70R-51295 |
Fitzgerald |
100 ul |
EUR 292.8 |
Description: Purified Polyclonal LIF antibody |
LIF antibody |
70R-6230 |
Fitzgerald |
50 ug |
EUR 560.4 |
Description: Rabbit polyclonal LIF antibody |
LIF Peptide |
43-200P |
ProSci |
0.1 mg |
EUR 405.6 |
LIF antibody |
70R-12299 |
Fitzgerald |
100 ug |
EUR 483.6 |
Description: Rabbit polyclonal LIF antibody |
LIF Antibody |
6245-002mg |
ProSci |
0.02 mg |
EUR 206.18 |
|
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation. |
LIF Antibody |
6245-01mg |
ProSci |
0.1 mg |
EUR 523.7 |
|
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation. |
LIF Peptide |
6245P |
ProSci |
0.05 mg |
EUR 197.7 |
Description: (IN) LIF peptide |
LIF antibody |
70R-14100 |
Fitzgerald |
100 ug |
EUR 386.4 |
Description: Affinity purified Rabbit polyclonal LIF antibody |
LIF Antibody |
36950-100ul |
SAB |
100ul |
EUR 302.4 |
LIF protein |
30R-2771 |
Fitzgerald |
10 ug |
EUR 442.8 |
Description: Purified recombinant Human LIF protein |
- This examine demonstrated that the generic analysis platform had nice potential for in-depth understanding of HDFs and HaCat cells cultivated in serum-free medium, which may inform the processes for manufacturing pores and skin cells or tissues for scientific purposes.
Comparability of Human Dermal Fibroblasts and HaCat Cells Cultured in Medium with or with out Serum by way of a Generic Tissue Engineering Analysis Platform.
- A generic analysis platform with 2-dimensional (2D) cell tradition know-how, a third-dimensional (3D) in vitro tissue mannequin, and a scaled-down cell tradition and imaging system in between, was utilized to deal with the problematic points related with using serum in pores and skin tissue engineering. Human dermal fibroblasts (HDFs) and immortalized keratinocytes (HaCat cells) mono- or co-cultured in serum or serum-free medium had been in contrast and analyzed by way of the platform.
Recombinant Mouse GRO/KC (CXCL1) |
7-01860 |
CHI Scientific |
1mg |
Ask for price |
Mouse CXCL1/KC ELISA Kit |
RK00038 |
Abclonal |
96 Tests |
EUR 625.2 |
GRO / KC (CXCL1) Protein |
20-abx260684 |
Abbexa |
-
EUR 4101.60
-
EUR 393.60
-
EUR 276.00
|
|
|
GRO / KC (CXCL1) Protein |
20-abx261870 |
Abbexa |
-
EUR 5388.00
-
EUR 393.60
-
EUR 276.00
|
|
|
GRO1/KC Mouse Recombinant Protein (CXCL1) |
PROTP12850-1 |
BosterBio |
Regular: 20ug |
EUR 380.4 |
Description: KC Mouse Recombinant also known as N51 and GRO1 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of approximately 8 kDa.;The GRO-1 is purified by proprietary chromatographic techniques. |
Recombinant Murine KC (CXCL1) Protein |
PROTP12850-2 |
BosterBio |
20ug |
EUR 380.4 |
Description: All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant murine KC is a 7.8 kDa protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2. |
GRO, KC, CXCL1, rRtGRO, rat |
RC352-12 |
Bio Basic |
5ug |
EUR 125.26 |
|
GRO/KC (CXCL1), Rat Recombinant |
P1288-25 |
Biovision |
each |
EUR 444 |
GRO/KC (CXCL1), Rat Recombinant |
P1288-5 |
Biovision |
each |
EUR 210 |
GRO1/KC Mouse, GRO/KC (CXCL1) Mouse Recombinant Protein, His Tag |
PROTP12850 |
BosterBio |
Regular: 20ug |
EUR 380.4 |
Description: GRO1/KC Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 97 amino acids (25-96 a.a.) and having a molecular mass of 10.5kDa.;GRO1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
GRO-alpha, KC, CXCL1 (rMuKC), murine (mouse) |
RC332-12 |
Bio Basic |
5ug |
EUR 125.26 |
|
Recombinant Rat GRO/KC (CXCL1) Protein |
PROTP14095-1 |
BosterBio |
25ug |
EUR 380.4 |
Description: All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant rat GRO/KC is a 7.8 kDa protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2. |
Rat CXCL1/CINC-1/KC ELISA Kit |
RK00196 |
Abclonal |
96 Tests |
EUR 625.2 |
KC protein (Mouse) |
30R-AK001 |
Fitzgerald |
20 ug |
EUR 327.6 |
Description: Purified recombinant Mouse KC protein |
Anti-CXCL1 antibody |
STJ28366 |
St John's Laboratory |
100 µl |
EUR 332.4 |
Description: This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. |
Rabbit Polyclonal antibody Anti-CRBN |
Anti-CRBN |
ImmunoStep |
50 µg |
EUR 418.8 |
KC antibody |
70R-KR006 |
Fitzgerald |
50 ug |
EUR 327.6 |
Description: Affinity purified Rabbit polyclonal KC antibody |
KC antibody |
70R-12297 |
Fitzgerald |
100 ug |
EUR 632.4 |
Description: Rabbit polyclonal KC antibody |
KC antibody |
70R-12298 |
Fitzgerald |
100 ug |
EUR 590.4 |
Description: Rabbit polyclonal KC antibody |
KC antibody |
20R-1786 |
Fitzgerald |
100 ug |
EUR 781.2 |
Description: Rabbit polyclonal KC antibody |
KC Antibody |
5211-100 |
Biovision |
each |
EUR 451.2 |
KC Antibody |
5212-100 |
Biovision |
each |
EUR 470.4 |
KC Antibody |
5212-30T |
Biovision |
each |
EUR 175.2 |
anti-7-ketocholesterol (7-KC) (35A) |
LF-MA90006 |
Abfrontier |
20 ug |
EUR 1230 |
Description: Mouse monoclonal to 7-ketocholesterol (7-KC) |
anti-7-ketocholesterol (7-KC) (35A) |
LF-MA90007 |
Abfrontier |
100 ug |
EUR 3270 |
Description: Mouse monoclonal to 7-ketocholesterol (7-KC) |
KC Recombinant Protein |
40-339-0005mg |
ProSci |
0.005 mg |
EUR 311.1 |
Description: All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant murine KC is a 7.8 kDa protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2. |
KC Recombinant Protein |
40-339-002mg |
ProSci |
0.02 mg |
EUR 437.1 |
Description: All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant murine KC is a 7.8 kDa protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2. |
KC Blocking Peptide |
33R-11041 |
Fitzgerald |
50 ug |
EUR 229.2 |
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of KC antibody, catalog no. 70R-12298 |
Polyclonal KC Antibody |
APR16969G |
Leading Biology |
0.1mg |
EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human KC . This antibody is tested and proven to work in the following applications: |
KC 12291 hydrochloride |
B7332-10 |
ApexBio |
10 mg |
EUR 447.6 |
KC 12291 hydrochloride |
B7332-50 |
ApexBio |
50 mg |
EUR 1635.6 |
KC Blocking Peptide |
5212BP-50 |
Biovision |
each |
EUR 183.6 |
Mouse CXCL1 shRNA Plasmid |
20-abx970673 |
Abbexa |
|
|
|
Mouse CXCL1 ELISA Kit |
55R-1741 |
Fitzgerald |
1 kit |
EUR 748.8 |
Description: ELISA kit for detection of CXCL1 in the research laboratory |
Anti-GRO alpha/Cxcl1 Antibody |
A00533 |
BosterBio |
100ug/vial |
EUR 352.8 |
Anti-GRO alpha/CXCL1 Antibody |
PA1760 |
BosterBio |
100ug/vial |
EUR 352.8 |
KiloGreen 2X qPCR MasterMix-iCycler |
MasterMix-KC |
ABM |
4 x 1.25 ml - 500 reactions (20 ul) |
EUR 168 |
Mouse keinocyte chemoattractant (KC) ELISA kit |
E03K0088-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse keinocyte chemoattractant (KC) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse keinocyte chemoattractant (KC) ELISA kit |
E03K0088-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse keinocyte chemoattractant (KC) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse keinocyte chemoattractant (KC) ELISA kit |
E03K0088-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse keinocyte chemoattractant (KC) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Cxcl1 Antibody |
1-CSB-PA09217A0Rb |
Cusabio |
|
|
|
Description: A polyclonal antibody against Cxcl1. Recognizes Cxcl1 from Mouse. This antibody is Unconjugated. Tested in the following application: ELISA |
CXCL1 Antibody |
1-CSB-PA06097A0Rb |
Cusabio |
|
|
|
Description: A polyclonal antibody against CXCL1. Recognizes CXCL1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA |
CXCL1 Antibody |
1-CSB-PA009036 |
Cusabio |
|
|
|
Description: A polyclonal antibody against CXCL1. Recognizes CXCL1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/10000 |
CXCL1 Antibody |
1-CSB-PA006239GA01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against CXCL1. Recognizes CXCL1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC |
CXCL1 Antibody |
43679-100ul |
SAB |
100ul |
EUR 302.4 |
CXCL1 antibody |
70R-10502 |
Fitzgerald |
50 ug |
EUR 560.4 |
Description: Affinity purified rabbit polyclonal CXCL1 antibody |
CXCL1 antibody |
70R-16681 |
Fitzgerald |
50 ul |
EUR 522 |
Description: Rabbit polyclonal CXCL1 antibody |
CXCL1 antibody |
70R-14297 |
Fitzgerald |
100 ug |
EUR 392.4 |
Description: Affinity purified Rabbit polyclonal CXCL1 antibody |
CXCL1 antibody |
70R-15473 |
Fitzgerald |
100 ug |
EUR 392.4 |
Description: Rabbit polyclonal CXCL1 antibody |
CXCL1 Peptide |
42-129P |
ProSci |
0.1 mg |
EUR 405.6 |
Description: (CT) CXCL1 Peptide |
CXCL1 protein |
30R-3156 |
Fitzgerald |
50 ug |
EUR 308.4 |
Description: Purified recombinant CXCL1 protein |
CXCL1 Antibody |
33054-100ul |
SAB |
100ul |
EUR 302.4 |
CXCL1 siRNA |
20-abx901350 |
Abbexa |
|
|
|
CXCL1 siRNA |
20-abx913248 |
Abbexa |
|
|
|
CXCL1 siRNA |
20-abx913249 |
Abbexa |
|
|
|
CXCL1/GRO |
E21-597 |
EnoGene |
10ug |
EUR 411.6 |
Polyclonal Goat anti-GST α-form |
GST-ANTI-1 |
Detroit R&D |
50 uL |
EUR 336 |
Polyclonal Goat anti-GST μ-form |
GST-ANTI-2 |
Detroit R&D |
50 uL |
EUR 336 |
Polyclonal Goat anti-GST p-form |
GST-ANTI-3 |
Detroit R&D |
50 uL |
EUR 336 |
GRO/KC, murine recombinant |
4210-100 |
Biovision |
each |
EUR 927.6 |
GRO/KC, murine recombinant |
4210-1000 |
Biovision |
each |
EUR 4627.2 |
GRO/KC, murine recombinant |
4210-20 |
Biovision |
each |
EUR 307.2 |
Mouse CXCL1 PicoKine ELISA Kit |
EK0723 |
BosterBio |
96 wells |
EUR 547.2 |
Description: For quantitative detection of mouse CXCL1 in cell culture supernates and serum. |
ELISA kit for Mouse CXCL1 |
EK5299 |
SAB |
96 tests |
EUR 663.6 |
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse CXCL1 in samples from serum, plasma, tissue homogenates and other biological fluids. |
Mouse CXCL1 Detection Assay Kit |
6725 |
Chondrex |
1 kit |
EUR 580.26 |
Description: Mouse CXCL1 Detection Assay Kit |
CXCL1 protein (Mouse) (His tag) |
80R-3368 |
Fitzgerald |
50 ug |
EUR 308.4 |
Description: Purified recombinant CXCL1 protein (Mouse) (His tag) |
CXCL1 protein (Mouse) (His tag) |
80R-3439 |
Fitzgerald |
50 ug |
EUR 308.4 |
Description: Purified recombinant CXCL1 protein (Mouse) (His tag) |
CXCL1 ELISA Kit (Mouse) (OKBB00312) |
OKBB00312 |
Aviva Systems Biology |
96 Tests |
EUR 652.8 |
Description: Description of target: Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function. The standard product used in this kit is recombinant mouse CXCL1, consisting of 77 amino acids with the molecular mass of 8KDa.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 1 pg/ml |
CXCL1 ELISA Kit (Mouse) (OKAN04583) |
OKAN04583 |
Aviva Systems Biology |
96 Wells |
EUR 950.4 |
Description: Description of target: This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung.;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 6.0 pg/mL |
CXCL1 ELISA Kit (Mouse) (OKCD05712) |
OKCD05712 |
Aviva Systems Biology |
96 Wells |
EUR 730.8 |
Description: Description of target: This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 6.0pg/mL |
Mouse CXCL1/GROα ELISA kit |
LF-EK50473 |
Abfrontier |
1×96T |
EUR 777.6 |
Cxcl1 ORF Vector (Mouse) (pORF) |
ORF042286 |
ABM |
1.0 ug DNA |
EUR 114 |
KC ELISA Kit (Mouse) : 96 Wells (OKAG00090) |
OKAG00090 |
Aviva Systems Biology |
96 Wells |
EUR 715.2 |
Description: Description of target: This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung.;Species reactivity: Mouse;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 8 pg/mL |
CXCL1 Conjugated Antibody |
C33054 |
SAB |
100ul |
EUR 476.4 |
- It was demonstrated that serum depletion had important affect on the attachment of HaCat cells onto tissue tradition plastic (TCP), porous substrates and cellulosic scaffolds, which was additional enhanced by the pre-seeded HDFs. The advanced constructions fashioned by the HDFs colonized inside the porous substrates and scaffolds not solely prevented the seeded HaCat cells from filtering via the open pores, but additionally acted as mobile substrates for HaCat cells to connect onto.
- When mono-cultured on TCP, each HDFs and HaCat cells had been much less proliferative in medium with out serum than with serum. Nonetheless, each cell sorts had been efficiently co-cultured in 2D utilizing serum-free medium if the preliminary cell seeding density was greater than 80,000 cells/cm² (with 1:1 ratio).
- Primarily based on the outcomes from 2D cultures, co-culture of each cell sorts on modular substrates with small open pores (125 μm) and cellulosic scaffolds with open pores of various sizes (50-300 µm) had been then carried out efficiently in serum-free medium. This examine demonstrated that the generic analysis platform had nice potential for in-depth understanding of HDFs and HaCat cells cultivated in serum-free medium, which may inform the processes for manufacturing pores and skin cells or tissues for scientific purposes.