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The application of serum free medium in the research of isolating cancer stem cells

The isolation and identification of most cancers stem cells are the important thing factors in exploring traits of most cancers stem cells at current. A number of species of most cancers stem cells, for example, retinoblastoma tumor stem cells, most cancers stem cells of melanoma of choroid, breast most cancers stem cells, lung most cancers stem cells, colon most cancers stem cells, and many others., have been remoted and cultured efficiently by serum free medium whereas their organic capabilities and traits are acquired.

 

This overview concentrate on the appliance of serum free medium within the analysis of isolating most cancers stem cells, each ocular and common, by way of offering basis for additional analysis on ocular most cancers stem cells.

 

A generic analysis platform with 2-dimensional (2D) cell tradition know-how, a third-dimensional (3D) in vitro tissue mannequin, and a scaled-down cell tradition and imaging system in between, was utilized to deal with the problematic points related to using serum in pores and skin tissue engineering. Human dermal fibroblasts (HDFs) and immortalized keratinocytes (HaCat cells) mono- or co-cultured in serum or serum-free medium had been in contrast and analyzed by way of the platform.

 

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  • It was demonstrated that serum depletion had important affect on the attachment of HaCat cells onto tissue tradition plastic (TCP), porous substrates and cellulosic scaffolds, which was additional enhanced by the pre-seeded HDFs. The advanced constructions fashioned by the HDFs colonized inside the porous substrates and scaffolds not solely prevented the seeded HaCat cells from filtering via the open pores, but additionally acted as mobile substrates for HaCat cells to connect onto.

 

  • When mono-cultured on TCP, each HDFs and HaCat cells had been much less proliferative in medium with out serum than with serum. Nonetheless, each cell sorts had been efficiently co-cultured in 2D utilizing serum-free medium if the preliminary cell seeding density was greater than 80,000 cells/cm² (with 1:1 ratio). Primarily based on the outcomes from 2D cultures, co-culture of each cell sorts on modular substrates with small open pores (125 μm) and cellulosic scaffolds with open pores of various sizes (50-300 µm) had been then carried out efficiently in serum-free medium.

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Mu-48Tests 48 Tests
EUR 613.2

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Mu-96Tests 96 Tests
EUR 850.8

anti-LIF

YF-PA12952 50 ug
EUR 435.6
Description: Mouse polyclonal to LIF

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-b-48T 48T
EUR 656.4
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-b-96T 96T
EUR 858
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-c-48T 48T
EUR 632.4
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-c-96T 96T
EUR 825.6
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-Hu-48T 48T
EUR 456
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-Hu-96T 96T
EUR 582
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-p-48T 48T
EUR 656.4
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-p-96T 96T
EUR 858
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-Ra-48T 48T
EUR 609.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-Ra-96T 96T
EUR 793.2
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-b-48Tests 48 Tests
EUR 666

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-b-96Tests 96 Tests
EUR 925.2

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-c-48Tests 48 Tests
EUR 639.6

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-c-96Tests 96 Tests
EUR 888

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-Hu-48Tests 48 Tests
EUR 440.4

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-Hu-96Tests 96 Tests
EUR 602.4

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-p-48Tests 48 Tests
EUR 666

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-p-96Tests 96 Tests
EUR 925.2

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-Ra-48Tests 48 Tests
EUR 613.2

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-Ra-96Tests 96 Tests
EUR 850.8

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-b-48Tests 48 Tests
EUR 696

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-b-96Tests 96 Tests
EUR 968.4

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-c-48Tests 48 Tests
EUR 668.4

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-c-96Tests 96 Tests
EUR 928.8

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Hu-48Tests 48 Tests
EUR 459.6

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Hu-96Tests 96 Tests
EUR 630

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-p-48Tests 48 Tests
EUR 696

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-p-96Tests 96 Tests
EUR 968.4

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Ra-48Tests 48 Tests
EUR 640.8

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Ra-96Tests 96 Tests
EUR 890.4

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 418.8

LIF, mouse

MO16102 100 ug
EUR 810

LIF, Mouse

HY-P7084 50ug
EUR 639.6

Anti-LIF Antibody

A01400 100ug/vial
EUR 352.8

Anti-LIF Antibody

PA1562 100ug/vial
EUR 352.8

anti- LIF antibody

LSMab09804 100 ug
EUR 463.2

anti- LIF antibody

FNab09804 100µg
EUR 658.5
Description: Antibody raised against LIF

Anti-LIF Antibody

PB9036 100ug/vial
EUR 400.8

Anti-LIF antibody

STJ29846 100 µl
EUR 332.4
Description: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Anti-LIF antibody

STJ73631 100 µg
EUR 430.8

Anti-LIF antibody

STJ96472 200 µl
EUR 236.4
Description: Rabbit polyclonal to LIF.

Mouse LIF shRNA Plasmid

20-abx971321
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse LIF ELISA Kit

EML0014 96Tests
EUR 625.2

Recombinant Mouse LIF Protein

R01400-1 100ug/vial
EUR 352.8

Anti-LIF (aa28-39) antibody

STJ72919 100 µg
EUR 430.8

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 336

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 336

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 336

LIF Antibody

1-CSB-PA198481
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100

LIF Antibody

1-CSB-PA004761
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

LIF Antibody

DF13730 100ul
EUR 420

rHu LIF

AK9977-0005 5µg Ask for price

rHu LIF

AK9977-0025 25µg Ask for price

rHu LIF

AK9977-0100 100µg Ask for price

rHu LIF

AK9977-1000 1mg Ask for price

LIF Antibody

1-CSB-PA012928ESR2HU
  • EUR 266.40
  • EUR 402.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: ELISA

LIF antibody

70R-51295 100 ul
EUR 292.8
Description: Purified Polyclonal LIF antibody

LIF antibody

70R-6230 50 ug
EUR 560.4
Description: Rabbit polyclonal LIF antibody

LIF Peptide

43-200P 0.1 mg
EUR 405.6

LIF antibody

70R-12299 100 ug
EUR 483.6
Description: Rabbit polyclonal LIF antibody

LIF Antibody

6245-002mg 0.02 mg
EUR 206.18
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

LIF Antibody

6245-01mg 0.1 mg
EUR 523.7
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

LIF Peptide

6245P 0.05 mg
EUR 197.7
Description: (IN) LIF peptide

LIF antibody

70R-14100 100 ug
EUR 386.4
Description: Affinity purified Rabbit polyclonal LIF antibody

LIF Antibody

36950-100ul 100ul
EUR 302.4

LIF protein

30R-2771 10 ug
EUR 442.8
Description: Purified recombinant Human LIF protein
  • This examine demonstrated that the generic analysis platform had nice potential for in-depth understanding of HDFs and HaCat cells cultivated in serum-free medium, which may inform the processes for manufacturing pores and skin cells or tissues for scientific purposes.

Comparability of Human Dermal Fibroblasts and HaCat Cells Cultured in Medium with or with out Serum by way of a Generic Tissue Engineering Analysis Platform.

  • A generic analysis platform with 2-dimensional (2D) cell tradition know-how, a third-dimensional (3D) in vitro tissue mannequin, and a scaled-down cell tradition and imaging system in between, was utilized to deal with the problematic points related with using serum in pores and skin tissue engineering. Human dermal fibroblasts (HDFs) and immortalized keratinocytes (HaCat cells) mono- or co-cultured in serum or serum-free medium had been in contrast and analyzed by way of the platform.

Recombinant Mouse GRO/KC (CXCL1)

7-01860 1mg Ask for price

Mouse CXCL1/KC ELISA Kit

RK00038 96 Tests
EUR 625.2

GRO / KC (CXCL1) Protein

20-abx260684
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

GRO / KC (CXCL1) Protein

20-abx261870
  • EUR 5388.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

GRO1/KC Mouse Recombinant Protein (CXCL1)

PROTP12850-1 Regular: 20ug
EUR 380.4
Description: KC Mouse Recombinant also known as N51 and GRO1 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of approximately 8 kDa.;The GRO-1 is purified by proprietary chromatographic techniques.

Recombinant Mouse (E.Coli) GRO/KC (CXCL1)

RP-1037 5 ug
EUR 196.8

Recombinant Murine KC (CXCL1) Protein

PROTP12850-2 20ug
EUR 380.4
Description: All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant murine KC is a 7.8 kDa protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2.

GRO, KC, CXCL1, rRtGRO, rat

RC352-12 5ug
EUR 125.26

GRO/KC (CXCL1), Rat Recombinant

P1288-25 each
EUR 444

GRO/KC (CXCL1), Rat Recombinant

P1288-5 each
EUR 210

GRO1/KC Mouse, GRO/KC (CXCL1) Mouse Recombinant Protein, His Tag

PROTP12850 Regular: 20ug
EUR 380.4
Description: GRO1/KC Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 97 amino acids (25-96 a.a.) and having a molecular mass of 10.5kDa.;GRO1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

GRO-alpha, KC, CXCL1 (rMuKC), murine (mouse)

RC332-12 5ug
EUR 125.26

Recombinant Rat GRO/KC (CXCL1) Protein

PROTP14095-1 25ug
EUR 380.4
Description: All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant rat GRO/KC is a 7.8 kDa protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2.

Rat CXCL1/CINC-1/KC ELISA Kit

RK00196 96 Tests
EUR 625.2

Mouse CXC-chemokine ligand 1(CXCL1/KC)ELISA Kit

YLA0122MO-48T 48T
EUR 465

Mouse CXC-chemokine ligand 1(CXCL1/KC)ELISA Kit

YLA0122MO-96T 96T
EUR 600

KC protein (Mouse)

30R-AK001 20 ug
EUR 327.6
Description: Purified recombinant Mouse KC protein

Anti-CXCL1 antibody

STJ28366 100 µl
EUR 332.4
Description: This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4.

Anti-CXCL1 antibody

STJ72026 100 µg
EUR 312

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 418.8

KC antibody

70R-KR006 50 ug
EUR 327.6
Description: Affinity purified Rabbit polyclonal KC antibody

KC antibody

70R-12297 100 ug
EUR 632.4
Description: Rabbit polyclonal KC antibody

KC antibody

70R-12298 100 ug
EUR 590.4
Description: Rabbit polyclonal KC antibody

KC antibody

20R-1786 100 ug
EUR 781.2
Description: Rabbit polyclonal KC antibody

KC Antibody

5211-100 each
EUR 451.2

KC Antibody

5212-100 each
EUR 470.4

KC Antibody

5212-30T each
EUR 175.2

Rabbit Anti Mouse Cxcl1 Polyclonal Antibody

CPBT-65100RM 0.1 mg
EUR 1057.2

anti-7-ketocholesterol (7-KC) (35A)

LF-MA90006 20 ug
EUR 1230
Description: Mouse monoclonal to 7-ketocholesterol (7-KC)

anti-7-ketocholesterol (7-KC) (35A)

LF-MA90007 100 ug
EUR 3270
Description: Mouse monoclonal to 7-ketocholesterol (7-KC)

KC Recombinant Protein

40-339-0005mg 0.005 mg
EUR 311.1
Description: All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant murine KC is a 7.8 kDa protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2.

KC Recombinant Protein

40-339-002mg 0.02 mg
EUR 437.1
Description: All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant murine KC is a 7.8 kDa protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2.

KC Blocking Peptide

33R-11041 50 ug
EUR 229.2
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of KC antibody, catalog no. 70R-12298

Polyclonal KC Antibody

APR16969G 0.1mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human KC . This antibody is tested and proven to work in the following applications:

KC 12291 hydrochloride

B7332-10 10 mg
EUR 447.6

KC 12291 hydrochloride

B7332-50 50 mg
EUR 1635.6

KC Blocking Peptide

5212BP-50 each
EUR 183.6

Mouse CXCL1 shRNA Plasmid

20-abx970673
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse CXCL1 ELISA Kit

55R-1741 1 kit
EUR 748.8
Description: ELISA kit for detection of CXCL1 in the research laboratory

GRO-alpha/CXCL1, Mouse

HY-P7188 50ug
EUR 639.6

Anti-GRO alpha/Cxcl1 Antibody

A00533 100ug/vial
EUR 352.8

Anti-GRO alpha/CXCL1 Antibody

PA1760 100ug/vial
EUR 352.8

KiloGreen 2X qPCR MasterMix-iCycler

MasterMix-KC 4 x 1.25 ml - 500 reactions (20 ul)
EUR 168

Mouse keinocyte chemoattractant (KC) ELISA kit

E03K0088-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse keinocyte chemoattractant (KC) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse keinocyte chemoattractant (KC) ELISA kit

E03K0088-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse keinocyte chemoattractant (KC) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse keinocyte chemoattractant (KC) ELISA kit

E03K0088-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse keinocyte chemoattractant (KC) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Cxcl1 Antibody

1-CSB-PA09217A0Rb
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Cxcl1. Recognizes Cxcl1 from Mouse. This antibody is Unconjugated. Tested in the following application: ELISA

CXCL1 Antibody

1-CSB-PA06097A0Rb
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against CXCL1. Recognizes CXCL1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA

CXCL1 Antibody

1-CSB-PA009036
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against CXCL1. Recognizes CXCL1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/10000

CXCL1 Antibody

1-CSB-PA006239GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against CXCL1. Recognizes CXCL1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

CXCL1 Antibody

43679-100ul 100ul
EUR 302.4

CXCL1 antibody

70R-10502 50 ug
EUR 560.4
Description: Affinity purified rabbit polyclonal CXCL1 antibody

CXCL1 antibody

70R-16681 50 ul
EUR 522
Description: Rabbit polyclonal CXCL1 antibody

CXCL1 antibody

70R-14297 100 ug
EUR 392.4
Description: Affinity purified Rabbit polyclonal CXCL1 antibody

CXCL1 antibody

70R-15473 100 ug
EUR 392.4
Description: Rabbit polyclonal CXCL1 antibody

CXCL1 Peptide

42-129P 0.1 mg
EUR 405.6
Description: (CT) CXCL1 Peptide

CXCL1 protein

30R-3156 50 ug
EUR 308.4
Description: Purified recombinant CXCL1 protein

CXCL1 Antibody

33054-100ul 100ul
EUR 302.4

CXCL1 siRNA

20-abx901350
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

CXCL1 siRNA

20-abx913248
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

CXCL1 siRNA

20-abx913249
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

CXCL1/GRO

E21-597 10ug
EUR 411.6

CXCL1/GRO

EF012822 96 Tests
EUR 826.8

pEGFP- CXCL1

PVT10178 2 ug
EUR 361.2

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 336

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 336

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 336

KC Kimtech precision wipes

CLE7552 PK30
EUR 209.76

KC Delicate Task Wipes

CLE7558 PK15
EUR 126.54

GRO/KC, murine recombinant

4210-100 each
EUR 927.6

GRO/KC, murine recombinant

4210-1000 each
EUR 4627.2

GRO/KC, murine recombinant

4210-20 each
EUR 307.2

Mouse CXCL1 PicoKine ELISA Kit

EK0723 96 wells
EUR 547.2
Description: For quantitative detection of mouse CXCL1 in cell culture supernates and serum.

ELISA kit for Mouse CXCL1

EK5299 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse CXCL1 in samples from serum, plasma, tissue homogenates and other biological fluids.

Mouse CXCL1 Detection Assay Kit

6725 1 kit
EUR 580.26
Description: Mouse CXCL1 Detection Assay Kit

CXCL1 protein (Mouse) (His tag)

80R-3368 50 ug
EUR 308.4
Description: Purified recombinant CXCL1 protein (Mouse) (His tag)

CXCL1 protein (Mouse) (His tag)

80R-3439 50 ug
EUR 308.4
Description: Purified recombinant CXCL1 protein (Mouse) (His tag)

CXCL1 ELISA Kit (Mouse) (OKBB00312)

OKBB00312 96 Tests
EUR 652.8
Description: Description of target: Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function. The standard product used in this kit is recombinant mouse CXCL1, consisting of 77 amino acids with the molecular mass of 8KDa.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 1 pg/ml

CXCL1 ELISA Kit (Mouse) (OKAN04583)

OKAN04583 96 Wells
EUR 950.4
Description: Description of target: This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung.;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 6.0 pg/mL

CXCL1 ELISA Kit (Mouse) (OKCD05712)

OKCD05712 96 Wells
EUR 730.8
Description: Description of target: This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 6.0pg/mL

Mouse CXCL1/GROα ELISA kit

LF-EK50473 1×96T
EUR 777.6

Cxcl1 ORF Vector (Mouse) (pORF)

ORF042286 1.0 ug DNA
EUR 114

Rabbit Anti Rat Cxcl1 Polyclonal Antibody

CPBT-65101RR 0.1 mg
EUR 1057.2

KC ELISA Kit (Mouse) : 96 Wells (OKAG00090)

OKAG00090 96 Wells
EUR 715.2
Description: Description of target: This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung.;Species reactivity: Mouse;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 8 pg/mL

CXCL1 Conjugated Antibody

C33054 100ul
EUR 476.4
  • It was demonstrated that serum depletion had important affect on the attachment of HaCat cells onto tissue tradition plastic (TCP), porous substrates and cellulosic scaffolds, which was additional enhanced by the pre-seeded HDFs. The advanced constructions fashioned by the HDFs colonized inside the porous substrates and scaffolds not solely prevented the seeded HaCat cells from filtering via the open pores, but additionally acted as mobile substrates for HaCat cells to connect onto.

 

  • When mono-cultured on TCP, each HDFs and HaCat cells had been much less proliferative in medium with out serum than with serum. Nonetheless, each cell sorts had been efficiently co-cultured in 2D utilizing serum-free medium if the preliminary cell seeding density was greater than 80,000 cells/cm² (with 1:1 ratio).

 

  • Primarily based on the outcomes from 2D cultures, co-culture of each cell sorts on modular substrates with small open pores (125 μm) and cellulosic scaffolds with open pores of various sizes (50-300 µm) had been then carried out efficiently in serum-free medium. This examine demonstrated that the generic analysis platform had nice potential for in-depth understanding of HDFs and HaCat cells cultivated in serum-free medium, which may inform the processes for manufacturing pores and skin cells or tissues for scientific purposes.

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