Essentially the most generally used histological stain, hematoxylin is a pure compound extracted from a species of tree present in Mexico and the West Indies. The extracted compound is oxidized to provide hematein, the energetic staining element of the hematoxylin stain.
The manufacturing of histological staining options is an artwork. Only some laboratories are able to creating a stain of dependable vibrance. At Nationwide Diagnostics we have now gone one step additional. Our proprietary strategies obtain the best management of the ripening course of, leading to the one commercially out there Harris’ Hematoxylin with a full one 12 months shelf life.
Histo-Clear II
Low Hazard
Biodegradable
Lowered Citrus Odor
Histo-Clear II, like its predecessor Histo-Clear, is a safer histological clearing agent that results in the manufacturing of high-quality tissue slides. Histo-Clear II is unhazardous and fully biodegradable, thus lowering disposal prices. Histo-Clear II has a vastly diminished citrus odor in comparison with different citrus-based histological clearing brokers.
Notice: Histo-Clear II is just not suitable with many artificial mounting media. Histo-Clear II is a long-standing favourite dry mounting procedures and aqueous mounting. Please affirm compatibility along with your mounting media earlier than utilizing Histo-Clear II with in wet-mounting with artificial media.
Hazardous Supplies Charge (HazMat) applies if shipped by Air.
PROCEDURE
Notice: The next protocol has given good to wonderful outcomes with all kinds of tissue sections. It
might should be optimized for a given tissue, part
thickness, and so forth.
Nationwide Diagnostics HISTO-CLEAR
1. Dewax and rehydrate slides
a) Immerse slides within the following sequence of baths:
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
l 100% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 50% ethanol – 2 minutes
l Water – 2 minutes
2. Stain
a) Immerse slides in Hematoxylin answer for 10- 20 minutes.
b) Rinse 2 minutes in water or Scott’s Tapwater substitute.
c) Differentiate with 2-5 dips in 70% ethanol containing 0.1% HCl.
d) Immerse for two minutes in Scott’s Tapwater substitute.
e) Stain in 1% aqueous Eosin with 0.1 acetic acid for 10 minutes.
3. Dehydrate, clear and mount
a) Immerse slides within the following sequence of
baths:
l 50% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 100% ethanol – 2 minutes
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
b) Mount coverslip with Histomount.
