Direct PCR

Direct PCR—Phire and Phusion Kits

What is Direct PCR?

Direct PCR (dPCR) is a method of DNA amplification directly from an animal or plant tissue sample without performing DNA isolation and purification steps. This technique greatly reduces experimental time, and cost in genotyping and high-volume projects. It also provides a better option when faced with the challenges of amplifying a very small quantity of sample where the purification step could potentially lead to sample loss.

In many ways, dPCR works like conventional PCR. Both techniques depend on template DNA, primers and a master mix or PCR reagents, and both use a thermal cycler for amplification. The primary difference between dPCR and conventional PCR is the tailored buffers used in dPCR. The modified elements of dPCR enable robust amplification despite the presence of PCR inhibitors often found in crude samples.

ALS SARS-CoV-2 RT-PCR 4G has been developed and produced entirely at ALS in Portugal and is designed mainly for export. This new kit is able to detect all known SARS-CoV-2 variants, including British, Brazilian and South African variants, without cross-reacting with other known related viruses or common respiratory pathogens. The choice of the genomic regions used as targets and the sequences of the primers and hydrolysis probes were based on WHO, CDC and DGS recommendations.

The ALS SARS-CoV-2 RT-PCR 4G kit is an in vitro diagnostic medical device (CE-IVD) that uses real-time PCR nucleic acid amplification technology for the detection of SARS-CoV-2 in clinical samples from the upper respiratory tract of individuals who may or may not be suspected of having COVID-19.

The kit uses the RT-PCR technique, combining amplification of certain regions of the viral genome with its detection by specific hydrolysis probes. The SARS-CoV-2 genome target regions are highly conserved and correspond to two specific genes: ORF polyprotein (ORF1ab gene) and nucleocapsid phosphoprotein (N gene). The kit can also detect the gene that codifies the structural protein of the envelope (gene E) specific to the Sarbecovirus subgenus.

Direct PCR Reagent
Direct PCR Reagent

Advantages

  • Rapid amplification: The amplification rate of the polymerase is 6kb/min. 1kb fragment can be amplified within 25min.
  • Long fragment amplification: For plasmid, λ DNA and other easy templates, the polymerase can effectively amplify > 20kb. For genome, the polymerase can effectively amplify > 8kb. And for cDNA, the polymerase can effectively amplify > 8kb.
  • High specificity: With hot start technology, the polymerase is 100% inactive below 50°C, and can only be restored by heating at 95°C for 5min.
  • High tolerance to impurities: Samples of whole blood, serum, cultured cells and urine can be directly amplified without prior DNA purification.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol dNTP into an acid insoluble substance within 30 min at 74°C.

Description Code Specification Category Technology
ALS SARS-CoV-2 RT-PCR Test for surfaces ALS-B01-Adv RUO Food /Water Virology Detection Real-Time PCR
ALS SARS-CoV-2 RT-PCR Test for Wastewater ALS-B02-Adv RUO Food /Water Virology Detection Real-Time PCR

 

Direct PCR Complete Kit

S700 100 rcs
EUR 159.6

Direct PCR Complete Kit

S700-2 5x100 rcs
EUR 628.8

amfiSure Direct PCR Lysis Buffer

D0300-010 100ml
EUR 165.6

Blood Direct PCR Kit V2

PD103-01 50 rxn
EUR 202.8

Blood Direct PCR Kit V2

PD103-02 200 rxn
EUR 424.8

Mouse Direct PCR Kit (For Genotyping)

B40013 200 ractions
EUR 250.8
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B40015 500 ractions
EUR 490.8
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B45012 1ml*5 (500 ractions)
EUR 336
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B45013 1ml*20 (2000 ractions)
EUR 1038
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

FastAmp Plant Direct PCR Kit - 1250 Reactions

4615 1/EA
EUR 621.6

Direct RT-PCR Kit wioRealStart DNA Polymerase

FYT507-100P 100 Preps Ask for price

Direct RT-PCR Kit wioRealStart DNA Polymerase

FYT507-50P 50 Preps Ask for price

PCR Tubes (50)

PCR-50 50
EUR 15
Description: To be used with the PractiChrom kits in conjuction with the PiCO Explorer. Quantity: 50 PCR tubes.

Allele-In-One Human Blood Direct PCR Kit

ABP-PP-BD02100 100 RXNS Ask for price

Allele-In-One Human Blood Direct PCR Kit

ABP-PP-BD02500 500 RXNS Ask for price

Allele-In-One Mouse Tail Direct PCR Kit

ABP-PP-MT02100 100 RXNS Ask for price

Allele-In-One Mouse Tail Direct PCR Kit

ABP-PP-MT02500 500 RXNS Ask for price

Direct RT-PCR Kit wi RealStart DNA Polymerase

FYT506-100P 100 Preps Ask for price

Direct RT-PCR Kit wi RealStart DNA Polymerase

FYT506-50P 50 Preps; Ask for price

MULTIPLEX KIT PCR MASTITIS PCR kit

PCR-MPX218-48D 50T
EUR 543.6

MULTIPLEX KIT PCR MASTITIS PCR kit

PCR-MPX218-96D 100T
EUR 686.4

MULTIPLEX KIT PCR Babesia & Theileria PCR kit

PCR-MPX401-48D 50T
EUR 543.6

MULTIPLEX KIT PCR Babesia & Theileria PCR kit

PCR-MPX401-96D 100T
EUR 686.4

ranavirus PCR kit

PCR-V337-48D 50T
EUR 543.6

ranavirus PCR kit

PCR-V337-96D 100T
EUR 686.4

Ruminococcus PCR kit

PCR-V619-48D 50T
EUR 510.96
Description: An conventional PCR kit for detection of Ruminococcus

 

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